FastQCFastQC Report
Thu 26 May 2016
SRR1545875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545875_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3024970
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280470.9271827489198241No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44930.14853039864858164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA254.4445685E-544.032
CTCGTCG302.529092E-644.021
TAGTCCG351.4471334E-744.01
CGTTTTT211050.042.999291
CGGTCTA2100.041.9047631
CGGGCAC1350.039.111116
TACGACG800.038.5000041
TAAGGGA60250.038.3037344
CGACGGT2300.038.26086828
GGCGATT15950.038.0689668
TAGGGAT84350.037.8446965
TCGTCGT357.2941493E-637.71428722
TATGGGA44300.037.0474054
TTAGGGA64850.037.0115664
ATAGGGA70300.036.9900444
TAGGGAC36550.036.837215
GTTTTTT257150.036.8306432
TTACGAG1800.036.6666681
TAGCGGA601.9826984E-1036.6666643
GTACGAT301.3015975E-436.66666439
Produced by FastQC (version 0.11.5)