##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545875_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3024970
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.502647960145058	31.0	31.0	33.0	30.0	34.0
2	31.91319682509248	31.0	31.0	34.0	30.0	34.0
3	31.968209271496907	33.0	31.0	34.0	30.0	34.0
4	35.725152976723734	37.0	35.0	37.0	33.0	37.0
5	35.65268448943295	37.0	35.0	37.0	33.0	37.0
6	35.70491641239417	37.0	35.0	37.0	33.0	37.0
7	36.00688238230462	37.0	35.0	37.0	35.0	37.0
8	35.99564227083244	37.0	35.0	37.0	35.0	37.0
9	37.76261714992215	39.0	38.0	39.0	35.0	39.0
10	37.29177843086047	39.0	37.0	39.0	34.0	39.0
11	37.113683772070466	39.0	37.0	39.0	33.0	39.0
12	36.68463819475895	39.0	35.0	39.0	33.0	39.0
13	36.47328799955041	39.0	35.0	39.0	32.0	39.0
14	37.62354370456566	40.0	36.0	41.0	33.0	41.0
15	37.71034820180035	40.0	36.0	41.0	33.0	41.0
16	37.7971391451816	40.0	36.0	41.0	33.0	41.0
17	37.70167935549774	40.0	36.0	41.0	33.0	41.0
18	37.571710463244266	39.0	36.0	41.0	33.0	41.0
19	37.4676978614664	39.0	36.0	41.0	33.0	41.0
20	37.282245113174675	39.0	35.0	41.0	32.0	41.0
21	37.16622049144289	39.0	35.0	41.0	32.0	41.0
22	37.0721303021187	39.0	35.0	41.0	32.0	41.0
23	36.98451786298707	39.0	35.0	41.0	32.0	41.0
24	37.038703193750685	39.0	35.0	41.0	32.0	41.0
25	37.001400013884435	39.0	35.0	41.0	32.0	41.0
26	36.76802282336684	39.0	35.0	41.0	31.0	41.0
27	36.762779465581474	39.0	35.0	41.0	31.0	41.0
28	36.734383481489076	38.0	35.0	41.0	31.0	41.0
29	36.700804636079035	38.0	35.0	41.0	31.0	41.0
30	36.575721081531384	38.0	35.0	41.0	31.0	41.0
31	36.425730503112426	38.0	35.0	41.0	30.0	41.0
32	36.20977827879285	38.0	35.0	41.0	30.0	41.0
33	35.94020965497179	38.0	35.0	41.0	29.0	41.0
34	35.66206904531285	38.0	35.0	41.0	27.0	41.0
35	35.40622287163178	38.0	35.0	41.0	25.0	41.0
36	35.25864223446844	38.0	35.0	41.0	24.0	41.0
37	35.164491548676516	38.0	34.0	40.0	24.0	41.0
38	35.081483452728456	38.0	34.0	40.0	24.0	41.0
39	34.972806341881075	38.0	34.0	40.0	23.0	41.0
40	34.862825747032204	38.0	34.0	40.0	23.0	41.0
41	34.81522791961573	38.0	34.0	40.0	23.0	41.0
42	34.80407607348172	38.0	34.0	40.0	23.0	41.0
43	34.711958135122	38.0	34.0	40.0	23.0	41.0
44	34.60641031150722	38.0	34.0	40.0	23.0	41.0
45	34.525341077762754	38.0	34.0	40.0	23.0	41.0
46	34.46490080893364	38.0	34.0	40.0	23.0	41.0
47	34.396152027953995	38.0	34.0	40.0	23.0	41.0
48	34.27203079699964	37.0	33.0	40.0	22.0	41.0
49	34.19999702476388	37.0	33.0	40.0	22.0	41.0
50	34.06881456675603	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	4.0
13	9.0
14	43.0
15	83.0
16	191.0
17	490.0
18	1275.0
19	2426.0
20	4204.0
21	6733.0
22	10297.0
23	15025.0
24	22560.0
25	34997.0
26	52853.0
27	65992.0
28	69284.0
29	68531.0
30	72547.0
31	83014.0
32	99076.0
33	125675.0
34	201321.0
35	310346.0
36	207213.0
37	271079.0
38	440233.0
39	859463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.719322836259533	22.985880851710927	29.04709798774864	16.2476983242809
2	33.39319067627117	24.98424777766391	29.615268911757802	12.007292634307117
3	27.69270439045676	24.537598719987304	35.06289318571754	12.706803703838384
4	24.879387233592396	27.044466556693123	34.748609077114814	13.327537132599662
5	23.031071382526108	31.79512524091148	32.39592458768186	12.777878788880551
6	20.344234818857707	40.900339507499254	28.476315467591412	10.27911020605163
7	85.86217383974056	3.133584795882273	9.58449174702559	1.419749617351577
8	86.84737369296225	2.5295457475611327	9.368026790348335	1.2550537691282888
9	80.79333018178693	6.628958303718714	10.549922809151827	2.0277887053425325
10	31.199251562825413	45.6995606567999	14.699120983018013	8.402066797356667
11	33.41233136196392	21.976548527753994	30.322218071584178	14.288902038697904
12	32.08107849003461	21.221003844666228	30.44116139994777	16.25675626535139
13	22.35552749283464	29.043792169839698	30.98972882375693	17.61095151356873
14	17.168699193711014	32.62452189608492	32.320122183029916	17.886656727174156
15	16.484560177456306	26.103167965302138	41.16553222015425	16.24673963708731
16	19.85890769164653	23.4236042010334	39.064387415412384	17.65310069190769
17	19.357745696651538	22.818507290981398	32.41876778943262	25.404979222934443
18	21.114027577132997	24.59809518772087	34.75409673484366	19.533780500302484
19	25.100215869909455	25.051355881215354	30.382879830213195	19.465548418662003
20	26.985358532481314	24.04205661543751	31.129102106797752	17.843482745283424
21	23.38039716096358	24.78771690297755	32.23787343345554	19.59401250260333
22	22.7453495406566	23.202378866567273	31.388906336261186	22.663365256514943
23	20.10294316968433	26.805224514623287	31.474361729207235	21.617470586485155
24	19.76174970330284	24.10850355540716	37.29713021947325	18.832616521816746
25	19.200289589648822	26.753686813422945	33.57312634505466	20.472897251873572
26	19.563235337871117	30.738089964528577	30.435012578637142	19.26366211896316
27	19.034668112411033	29.397217162484257	32.201740843710844	19.366373881393866
28	17.3962716985623	27.449495366896198	36.55798239321382	18.596250541327684
29	19.246604098553043	23.850021653107305	35.90042215294697	21.002952095392683
30	19.54505333937196	27.128831029729223	33.6333914055346	19.69272422536422
31	24.02420519872924	26.32826110672172	29.80928736483337	19.838246329715666
32	24.073164361960615	26.32568256875275	30.85283490414781	18.748318165138826
33	24.197859813485753	27.53904997404933	29.1667355378731	19.096354674591815
34	20.29137479049379	27.711250028925903	30.91802563331207	21.079349547268237
35	21.247714853370447	26.54872610306879	31.00946455667329	21.194094486887476
36	24.26784397861797	27.658555291457436	28.98587424007511	19.087726489849484
37	21.423121551618692	30.592204220207144	29.688360545724418	18.296313682449743
38	20.360466384790595	29.591202557380736	28.536580528071354	21.51175052975732
39	21.87707646687405	29.055858405207324	29.111263913361125	19.9558012145575
40	22.454305331953705	27.292700423475274	29.160520600204297	21.09247364436672
41	19.3192659761915	27.462553347636504	29.90006512461281	23.318115551559192
42	21.584412407395774	27.471644346886087	27.81158160246217	23.132361643255965
43	22.074995785082166	27.894425399260157	28.375851661338793	21.654727154318888
44	20.52466636032754	29.35166299169909	28.913146246078476	21.210524401894894
45	19.26594313332033	31.61287549959173	27.162186732430406	21.958994634657532
46	21.1404741204045	30.880174018254724	28.022922541380574	19.956429319960197
47	21.400046942614306	28.129336819869287	29.16316525453145	21.307450982984953
48	21.717438520051438	26.136722017077858	30.91346360459773	21.232375858272974
49	20.48896352691101	27.087111607718423	30.68132907103211	21.742595794338456
50	19.484887453429288	29.67679018304314	29.85510600105125	20.983216362476323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	872.0
1	2569.0
2	4266.0
3	30123.5
4	55981.0
5	40971.5
6	25962.0
7	25151.5
8	24341.0
9	23856.0
10	23371.0
11	22656.0
12	21941.0
13	20823.0
14	19705.0
15	18425.0
16	17145.0
17	16244.5
18	15344.0
19	14954.0
20	14564.0
21	15459.0
22	16354.0
23	18779.5
24	21205.0
25	24568.0
26	27931.0
27	34710.0
28	41489.0
29	52673.0
30	63857.0
31	72139.5
32	80422.0
33	88559.5
34	96697.0
35	107832.0
36	118967.0
37	124527.0
38	130087.0
39	142648.5
40	155210.0
41	181206.5
42	207203.0
43	221353.5
44	235504.0
45	249315.5
46	263127.0
47	244744.5
48	226362.0
49	219797.5
50	213233.0
51	199107.0
52	184981.0
53	170476.5
54	155972.0
55	141173.0
56	126374.0
57	118605.5
58	110837.0
59	105623.5
60	100410.0
61	90066.5
62	79723.0
63	66581.5
64	53440.0
65	43708.5
66	33977.0
67	27909.0
68	21841.0
69	17969.5
70	14098.0
71	11752.5
72	9407.0
73	7554.0
74	5701.0
75	4680.0
76	3659.0
77	2721.5
78	1784.0
79	1331.0
80	878.0
81	598.5
82	319.0
83	235.0
84	151.0
85	143.5
86	136.0
87	112.0
88	88.0
89	62.5
90	37.0
91	23.0
92	9.0
93	6.5
94	4.0
95	3.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3024970.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.397404080649874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.19677241859169	13.778182516495063
2	7.718898498979181	2.6857759248852515
3	2.6755884167362036	1.3964487851839789
4	1.3436990993567997	0.9350750477726217
5	0.7631913990060157	0.6638774579692072
6	0.5826070264384718	0.6081509915505575
7	0.4198133180380709	0.511256335264269
8	0.33488732244247965	0.4660936056014964
9	0.2658613602645427	0.41627677625581333
>10	3.7396089646369424	15.95224865673614
>50	1.4767799343530583	18.421096494852122
>100	1.4765058082780966	42.34787310724102
>500	0.0046291463027256004	0.4898213644822242
>1k	9.644054797345001E-4	0.3866692870403966
>5k	0.0	0.0
>10k+	1.928810959469E-4	0.9411536486697912
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28047	0.9271827489198241	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4493	0.14853039864858164	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02733911410691676	0.0
2	0.0	0.0	0.0	0.09143892336122342	0.0
3	3.30581790893794E-5	0.0	0.0	0.12472850970422847	0.0
4	3.30581790893794E-5	0.0	0.0	0.18105964687253095	0.0
5	3.30581790893794E-5	0.0	0.0	0.3238379223595606	0.0
6	3.30581790893794E-5	0.0	0.0	0.45051686463006246	0.0
7	3.30581790893794E-5	0.0	0.0	0.5381871555750967	0.0
8	3.30581790893794E-5	0.0	0.0	0.7711812017970426	0.0
9	3.30581790893794E-5	0.0	0.0	0.8696945754833932	0.0
10	3.30581790893794E-5	0.0	0.0	1.0628865740817264	0.0
11	3.30581790893794E-5	0.0	0.0	1.2345576980928736	0.0
12	3.30581790893794E-5	0.0	0.0	1.3981626264062124	0.0
13	3.30581790893794E-5	0.0	0.0	1.458427686886151	0.0
14	3.30581790893794E-5	0.0	0.0	1.4808080741296608	0.0
15	3.30581790893794E-5	0.0	0.0	1.5200811908878435	0.0
16	3.30581790893794E-5	0.0	0.0	1.6032225112976326	0.0
17	3.30581790893794E-5	0.0	0.0	1.7189261381104606	0.0
18	3.30581790893794E-5	0.0	0.0	1.8634895552683166	0.0
19	3.30581790893794E-5	0.0	0.0	1.9405812289047495	0.0
20	3.30581790893794E-5	0.0	0.0	2.030367243311504	0.0
21	3.30581790893794E-5	0.0	0.0	2.1434923321553603	0.0
22	3.30581790893794E-5	0.0	0.0	2.2797912045408717	0.0
23	3.30581790893794E-5	0.0	0.0	2.4176438113435834	0.0
24	3.30581790893794E-5	0.0	0.0	2.5194299447597825	0.0
25	3.30581790893794E-5	0.0	0.0	2.603166312393181	0.0
26	3.30581790893794E-5	0.0	0.0	2.6891506362046567	0.0
27	3.30581790893794E-5	0.0	0.0	2.781184606789489	0.0
28	3.30581790893794E-5	0.0	0.0	2.883764136503833	0.0
29	3.30581790893794E-5	0.0	0.0	2.9915668585142994	0.0
30	3.30581790893794E-5	0.0	0.0	3.121816084126454	0.0
31	3.30581790893794E-5	0.0	0.0	3.2417511578627227	0.0
32	3.30581790893794E-5	0.0	0.0	3.3593060427045556	0.0
33	3.30581790893794E-5	0.0	0.0	3.4746790877264897	0.0
34	3.30581790893794E-5	0.0	0.0	3.599209248356182	0.0
35	3.30581790893794E-5	0.0	0.0	3.7462520289457415	0.0
36	3.30581790893794E-5	0.0	0.0	3.881592214137661	0.0
37	3.30581790893794E-5	0.0	0.0	4.020469624492144	0.0
38	3.30581790893794E-5	0.0	0.0	4.159809849353878	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	4.4445685E-5	44.0	32
CTCGTCG	30	2.529092E-6	44.0	21
TAGTCCG	35	1.4471334E-7	44.0	1
CGTTTTT	21105	0.0	42.99929	1
CGGTCTA	210	0.0	41.90476	31
CGGGCAC	135	0.0	39.11111	6
TACGACG	80	0.0	38.500004	1
TAAGGGA	6025	0.0	38.303734	4
CGACGGT	230	0.0	38.260868	28
GGCGATT	1595	0.0	38.068966	8
TAGGGAT	8435	0.0	37.844696	5
TCGTCGT	35	7.2941493E-6	37.714287	22
TATGGGA	4430	0.0	37.047405	4
TTAGGGA	6485	0.0	37.011566	4
ATAGGGA	7030	0.0	36.990044	4
TAGGGAC	3655	0.0	36.83721	5
GTTTTTT	25715	0.0	36.830643	2
TTACGAG	180	0.0	36.666668	1
TAGCGGA	60	1.9826984E-10	36.666664	3
GTACGAT	30	1.3015975E-4	36.666664	39
>>END_MODULE