FastQCFastQC Report
Thu 26 May 2016
SRR1545874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545874_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2176903
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209530.9625141772508927No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25210.11580672175103807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG254.4441564E-544.01
CGTTTTT119250.042.228931
GCGTAAG1250.040.481
CGACGGT3000.039.60000228
CGGGATA4300.039.395356
GGCGATA7850.038.9554148
CGCCTAT404.126814E-738.519
TAGGGAC23300.038.4291845
CGGTCTA3050.038.22950431
GGGCGAT36850.037.0746277
TAGGGAT32600.036.8466265
ACTACCG301.3014775E-436.6666681
TCACGAC3250.036.55384425
AACGGGA7050.036.510644
CGAGGGA17950.036.4011154
TATGGGA26550.036.3766484
ATAGGGA35500.036.315494
TAAGGGA31450.036.3052444
TCGATAG850.036.235291
ACGGGAT8700.036.160925
Produced by FastQC (version 0.11.5)