##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545874_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2176903
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48232741651787	31.0	31.0	33.0	30.0	34.0
2	31.892885443219104	31.0	31.0	34.0	30.0	34.0
3	31.984207840220716	33.0	31.0	34.0	30.0	34.0
4	35.75379977886015	37.0	35.0	37.0	35.0	37.0
5	35.67697642017122	37.0	35.0	37.0	33.0	37.0
6	35.71407499553264	37.0	35.0	37.0	33.0	37.0
7	35.96261753509458	37.0	35.0	37.0	35.0	37.0
8	35.940828323540366	37.0	35.0	37.0	35.0	37.0
9	37.70792175857169	39.0	38.0	39.0	35.0	39.0
10	37.23976171653032	39.0	37.0	39.0	34.0	39.0
11	37.0061022470914	39.0	37.0	39.0	33.0	39.0
12	36.53562423314222	39.0	35.0	39.0	32.0	39.0
13	36.33154026614874	39.0	35.0	39.0	32.0	39.0
14	37.367809222551486	40.0	35.0	41.0	32.0	41.0
15	37.49263839500428	40.0	35.0	41.0	32.0	41.0
16	37.585884626003086	40.0	35.0	41.0	33.0	41.0
17	37.5196138734707	39.0	35.0	41.0	32.0	41.0
18	37.397347516173205	39.0	36.0	41.0	32.0	41.0
19	37.30474577875082	39.0	35.0	41.0	32.0	41.0
20	37.121336596072496	39.0	35.0	41.0	32.0	41.0
21	36.997930546285254	39.0	35.0	41.0	32.0	41.0
22	36.9058465168177	38.0	35.0	41.0	32.0	41.0
23	36.78835575126682	38.0	35.0	41.0	31.0	41.0
24	36.8599482843287	38.0	35.0	41.0	32.0	41.0
25	36.81254010858545	38.0	35.0	41.0	31.0	41.0
26	36.578347312673095	38.0	35.0	41.0	31.0	41.0
27	36.557738677377905	38.0	35.0	41.0	31.0	41.0
28	36.5232722817691	38.0	35.0	41.0	31.0	41.0
29	36.46168984102645	38.0	35.0	41.0	31.0	41.0
30	36.33614175734978	38.0	35.0	40.0	30.0	41.0
31	36.17278491508349	38.0	35.0	41.0	30.0	41.0
32	36.00632182508821	38.0	35.0	41.0	30.0	41.0
33	35.7611905537362	38.0	35.0	41.0	29.0	41.0
34	35.498598237955484	38.0	35.0	40.0	27.0	41.0
35	35.22785213672819	38.0	34.0	40.0	25.0	41.0
36	35.08127417712227	38.0	34.0	40.0	25.0	41.0
37	35.00319995884061	38.0	34.0	40.0	24.0	41.0
38	34.91119264386148	38.0	34.0	40.0	24.0	41.0
39	34.77161958984851	38.0	34.0	40.0	23.0	41.0
40	34.63909094709319	38.0	34.0	40.0	23.0	41.0
41	34.56252850953855	37.0	34.0	40.0	23.0	41.0
42	34.54590121838226	37.0	34.0	40.0	23.0	41.0
43	34.44409879539879	37.0	33.0	40.0	23.0	41.0
44	34.314464172266746	37.0	33.0	40.0	23.0	41.0
45	34.22558561405814	37.0	33.0	40.0	23.0	41.0
46	34.166412100125726	36.0	33.0	40.0	23.0	41.0
47	34.09621834321511	36.0	33.0	40.0	23.0	41.0
48	33.95244942011656	36.0	33.0	40.0	23.0	41.0
49	33.8699055493056	36.0	33.0	40.0	22.0	41.0
50	33.741551644698916	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	5.0
13	16.0
14	24.0
15	69.0
16	208.0
17	465.0
18	992.0
19	1825.0
20	3282.0
21	5335.0
22	7989.0
23	11786.0
24	16714.0
25	25153.0
26	36992.0
27	45887.0
28	49396.0
29	51009.0
30	54957.0
31	64200.0
32	77208.0
33	99077.0
34	163097.0
35	234437.0
36	159856.0
37	207093.0
38	315058.0
39	544765.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.3373145243495	21.946223602980933	29.064501266248428	15.65196060642114
2	32.97620518691003	24.691959173192373	29.76007658586533	12.571759054032263
3	28.02807474655508	24.74598087282713	34.09862543255257	13.127318948065211
4	25.80487049721554	26.835141483106966	33.247829600124575	14.112158419552914
5	23.518824678913116	31.278380341246258	31.40553345739337	13.797261522447258
6	21.64809364496259	40.15006640167247	27.953794909557296	10.248045043807648
7	85.66803389953526	3.714543091722507	8.927545232837659	1.6898777759045764
8	86.35910741084926	3.0695442102840595	8.775126866011025	1.7962215128556485
9	80.73823225012782	5.912895521757285	10.513789544136785	2.83508268397811
10	39.28466266066977	34.529099367312185	15.008707324120552	11.177530647897495
11	36.04138539934944	21.255931017597018	26.262217471334274	16.440466111719264
12	32.65138593681023	20.180641948676627	29.33676879493482	17.831203319578318
13	23.98682899513667	26.913280012935807	30.868486101585603	18.23140489034192
14	18.521036536768058	29.34862049434449	33.15370505713851	18.976637911748938
15	18.319649520442574	22.582126994174754	40.88239117682322	18.215832308559456
16	22.03217139211072	20.728300709769798	37.26550976318192	19.97401813493757
17	21.938138722763487	20.868913314006182	31.122011407949735	26.0709365552806
18	23.596595714186623	22.109620869648303	33.16261680010547	21.131166616059605
19	26.88305358575922	23.23406233534521	28.751901210113633	21.130982868781935
20	27.601781062362445	23.315692063449774	28.507701078091213	20.574825796096565
21	25.455475048727482	23.603302489821548	30.61992197171854	20.321300489732433
22	24.580240828369476	21.21812501521657	30.526578354662565	23.675055801751387
23	22.089270858646433	24.67275758267594	30.358771153331134	22.879200405346495
24	22.274350304078776	22.678272757215183	34.44806681786005	20.59931012084599
25	21.87281656555207	24.68075058925455	31.75286174900765	21.693571096185728
26	21.537064352430953	26.66636960856777	29.63600123661918	22.160564802382098
27	20.085277111566295	26.12946006321825	31.940054288133187	21.845208537082268
28	19.618053721272837	24.589703813169443	34.64637606728458	21.145866398273146
29	20.852973237668376	23.204708707737552	34.12196133681657	21.820356717777504
30	22.717456864178146	24.53664678674245	31.590704776464545	21.155191572614857
31	26.771610861852825	23.885124876946744	28.367180347493665	20.976083913706766
32	26.962202725615242	23.71240243593766	29.040889741067932	20.284505097379167
33	25.812541946058232	24.741846559079573	28.670455229286745	20.775156265575454
34	22.45359577344512	24.925593836748813	30.567508060763387	22.053302329042683
35	22.820401276492337	24.895826777766395	31.120311745631295	21.163460200109974
36	26.381377580902775	26.07213091258545	27.356019078479843	20.19047242803193
37	22.23291529296436	28.9110263525752	28.743816329896188	20.112242024564257
38	23.222578130490888	28.17309728545553	26.622821503760157	21.981503080293425
39	22.863352202647523	26.950213215747326	27.582166040471257	22.604268541133894
40	24.650248541161456	25.467832053150737	27.46291405726392	22.419005348423884
41	20.70652665736599	25.430117924409124	28.096061239292702	25.767294178932183
42	22.51845856246236	25.35804305474337	27.226293500445358	24.897204882348912
43	22.862249718981506	25.21127491670506	28.422809835808028	23.503665528505405
44	21.68681838373138	26.61083199389224	29.101296658601694	22.601052963774684
45	21.44045922119635	28.50260209113589	26.56797294137589	23.488965746291864
46	22.39465883413271	27.358683414006045	28.646384335912074	21.60027341594917
47	22.60183388970478	25.958758842263528	29.684832075659777	21.754575192371917
48	22.93542707231328	24.49769236387657	30.69874955383864	21.868131009971506
49	22.1151792247978	24.417532613993366	30.80605796399748	22.66123019721136
50	21.116099339290724	26.688235534610406	29.740507500793555	22.455157625305308
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	582.0
1	2240.5
2	3899.0
3	21029.0
4	38159.0
5	26958.0
6	15757.0
7	15032.0
8	14307.0
9	14212.0
10	14117.0
11	13957.5
12	13798.0
13	12784.5
14	11771.0
15	10636.5
16	9502.0
17	9023.0
18	8544.0
19	8111.5
20	7679.0
21	8046.0
22	8413.0
23	9284.5
24	10156.0
25	12949.5
26	15743.0
27	17413.5
28	19084.0
29	24544.0
30	30004.0
31	35374.0
32	40744.0
33	45903.0
34	51062.0
35	56047.5
36	61033.0
37	63366.5
38	65700.0
39	77243.5
40	88787.0
41	106916.5
42	125046.0
43	138539.5
44	152033.0
45	160207.0
46	168381.0
47	166672.0
48	164963.0
49	158076.5
50	151190.0
51	141920.5
52	132651.0
53	130342.0
54	128033.0
55	125024.0
56	122015.0
57	118464.5
58	114914.0
59	109477.0
60	104040.0
61	95537.5
62	87035.0
63	75654.0
64	64273.0
65	55606.5
66	46940.0
67	38211.0
68	29482.0
69	25864.0
70	22246.0
71	18182.5
72	14119.0
73	11401.5
74	8684.0
75	7401.0
76	6118.0
77	4501.5
78	2885.0
79	2144.0
80	1403.0
81	1113.5
82	824.0
83	610.5
84	397.0
85	319.0
86	241.0
87	162.0
88	83.0
89	61.5
90	40.0
91	28.0
92	16.0
93	11.0
94	6.0
95	3.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2176903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.980111333341217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77677054792471	14.951918754806865
2	6.879866411934326	2.611612609140565
3	2.431743016047898	1.3846425953599222
4	1.348141949482742	1.0235153717732046
5	0.8760479069884669	0.8313743403990828
6	0.6458436376095728	0.7354910487455862
7	0.4854225296744133	0.6449361559852748
8	0.41938609138694555	0.6367995764943233
9	0.3492903006747106	0.5966611915016014
>10	4.952008685776397	23.033734291830427
>50	1.8311358290754605	24.904854225774173
>100	1.00091279257899	27.1385700546195
>500	0.0024502148896666863	0.3192592373368632
>1k	7.350644669000059E-4	0.21220391842215683
>5k	0.0	0.0
>10k+	2.4502148896666864E-4	0.9744266278105308
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20953	0.9625141772508927	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2521	0.11580672175103807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03390137272997465	0.0
2	0.0	0.0	0.0	0.11194802891998404	0.0
3	0.0	0.0	0.0	0.15912514246156123	0.0
4	0.0	0.0	0.0	0.2334049794593512	0.0
5	0.0	0.0	0.0	0.40451963178883027	0.0
6	0.0	0.0	0.0	0.5763692732289863	0.0
7	0.0	0.0	0.0	0.7023739688906672	0.0
8	0.0	0.0	0.0	1.0447410840078772	0.0
9	0.0	0.0	0.0	1.1986753658752825	0.0
10	0.0	0.0	0.0	1.47585813423933	0.0
11	0.0	0.0	0.0	1.6884077976832224	0.0
12	0.0	0.0	0.0	1.90155463977954	0.0
13	0.0	0.0	0.0	1.97702883408218	0.0
14	0.0	0.0	0.0	2.0092764813131314	0.0
15	0.0	0.0	0.0	2.0554889216469454	0.0
16	0.0	0.0	0.0	2.1526452947145556	0.0
17	0.0	0.0	0.0	2.2709326047141283	0.0
18	0.0	0.0	0.0	2.434467681839751	0.0
19	0.0	0.0	0.0	2.5144436844452875	0.0
20	0.0	0.0	0.0	2.6156884344410383	0.0
21	0.0	0.0	0.0	2.737972247729917	0.0
22	0.0	0.0	0.0	2.8803763879235778	0.0
23	0.0	0.0	0.0	3.014787521538626	0.0
24	0.0	0.0	0.0	3.1241171517518236	0.0
25	0.0	0.0	0.0	3.2141073809903333	0.0
26	4.593681941730982E-5	0.0	0.0	3.302719505646324	0.0
27	4.593681941730982E-5	0.0	0.0	3.4023564669624693	0.0
28	4.593681941730982E-5	0.0	0.0	3.5021771755562834	0.0
29	9.187363883461964E-5	0.0	0.0	3.622531642429635	0.0
30	9.187363883461964E-5	0.0	0.0	3.76452235124854	0.0
31	9.187363883461964E-5	0.0	0.0	3.8831312189840337	0.0
32	9.187363883461964E-5	0.0	0.0	3.992874280571987	0.0
33	9.187363883461964E-5	0.0	0.0	4.1141015470142674	0.0
34	9.187363883461964E-5	0.0	0.0	4.2438271250487505	0.0
35	9.187363883461964E-5	0.0	0.0	4.39307585133559	0.0
36	9.187363883461964E-5	0.0	0.0	4.517380884678831	0.0
37	9.187363883461964E-5	0.0	0.0	4.6486223777540845	0.0
38	9.187363883461964E-5	0.0	0.0	4.809906550728259	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	25	4.4441564E-5	44.0	1
CGTTTTT	11925	0.0	42.22893	1
GCGTAAG	125	0.0	40.48	1
CGACGGT	300	0.0	39.600002	28
CGGGATA	430	0.0	39.39535	6
GGCGATA	785	0.0	38.955414	8
CGCCTAT	40	4.126814E-7	38.5	19
TAGGGAC	2330	0.0	38.429184	5
CGGTCTA	305	0.0	38.229504	31
GGGCGAT	3685	0.0	37.074627	7
TAGGGAT	3260	0.0	36.846626	5
ACTACCG	30	1.3014775E-4	36.666668	1
TCACGAC	325	0.0	36.553844	25
AACGGGA	705	0.0	36.51064	4
CGAGGGA	1795	0.0	36.401115	4
TATGGGA	2655	0.0	36.376648	4
ATAGGGA	3550	0.0	36.31549	4
TAAGGGA	3145	0.0	36.305244	4
TCGATAG	85	0.0	36.23529	1
ACGGGAT	870	0.0	36.16092	5
>>END_MODULE