FastQCFastQC Report
Thu 26 May 2016
SRR1545873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545873_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences6987530
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT799411.1440523332279076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT538600.043.0401041
CGTAAGG10050.040.05972
TACGGGA41000.038.419514
AGGGCGA53650.037.8490226
CGGTCTA9450.037.71428731
GCGTAAG5600.037.7142871
ATTAGCG4500.037.6444441
GTTTTTT641000.037.441192
TAGGTCG1000.037.41
ATAGGGA132600.037.131224
TAGGGAT151650.036.8770185
CGACGGT9650.036.7046628
ACGGGAT44700.036.6666685
TTAGGGA134000.036.5955244
TCACGAC9950.036.4824125
TATGGGA105000.036.4780924
TAGGGAA106500.036.4600945
GACCGAT27450.036.2258649
CGGGTAT5500.036.06
CGAGGGA58950.035.938934
Produced by FastQC (version 0.11.5)