FastQCFastQC Report
Thu 26 May 2016
SRR1545872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545872_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1199132
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110830.9242518755232952No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13910.11600057374834465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATC207.8566186E-444.031
TTCACGC207.8566186E-444.020
TACGCAC302.5278077E-644.02
TCATCGT254.4429562E-544.017
CGCCCAT254.4429562E-544.043
CGTTTTT69050.042.8530041
CGGTCTA1450.042.4827631
CGTTTGG1050.039.809522
CGTTAGG1500.039.62
ATTAGCG850.038.8235321
CGCATGG800.038.52
CTCGCGA357.290473E-637.71428314
TAGGGAT19950.037.4937325
ATGGGCG3600.037.277785
TACGGGA5800.037.1724174
TATAGCG950.037.052631
TAAGGGA19250.036.9142884
CGAGGGA8300.036.8433764
AGGGATT23250.036.713986
TATACGG601.9826984E-1036.6666642
Produced by FastQC (version 0.11.5)