##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545872_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1199132
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.473302355370386	31.0	31.0	33.0	30.0	34.0
2	31.888202466450732	31.0	31.0	34.0	30.0	34.0
3	31.978187555665265	33.0	31.0	34.0	30.0	34.0
4	35.737581016935586	37.0	35.0	37.0	35.0	37.0
5	35.66975695753261	37.0	35.0	37.0	33.0	37.0
6	35.71631646891252	37.0	35.0	37.0	33.0	37.0
7	35.96614968160303	37.0	35.0	37.0	35.0	37.0
8	35.94077299246455	37.0	35.0	37.0	35.0	37.0
9	37.70834236764593	39.0	38.0	39.0	35.0	39.0
10	37.24489130471041	39.0	37.0	39.0	34.0	39.0
11	37.02564855245294	39.0	37.0	39.0	33.0	39.0
12	36.5987606035032	39.0	35.0	39.0	32.0	39.0
13	36.40616212393631	39.0	35.0	39.0	32.0	39.0
14	37.4772810666382	40.0	36.0	41.0	32.0	41.0
15	37.597603933511905	40.0	36.0	41.0	32.0	41.0
16	37.68110683394322	40.0	35.0	41.0	33.0	41.0
17	37.59197402788017	40.0	35.0	41.0	32.0	41.0
18	37.470928138019836	39.0	36.0	41.0	32.0	41.0
19	37.395013226233644	39.0	36.0	41.0	32.0	41.0
20	37.228414386406165	39.0	35.0	41.0	32.0	41.0
21	37.106404465897	39.0	35.0	41.0	32.0	41.0
22	37.02344028847533	39.0	35.0	41.0	32.0	41.0
23	36.905381559327914	39.0	35.0	41.0	31.0	41.0
24	36.98008225950104	39.0	35.0	41.0	32.0	41.0
25	36.93591197632954	39.0	35.0	41.0	32.0	41.0
26	36.689483726562216	38.0	35.0	41.0	31.0	41.0
27	36.68967303015848	38.0	35.0	41.0	31.0	41.0
28	36.64865586107284	38.0	35.0	41.0	31.0	41.0
29	36.5878802333688	38.0	35.0	41.0	31.0	41.0
30	36.47074217016976	38.0	35.0	41.0	30.0	41.0
31	36.33239876844251	38.0	35.0	41.0	30.0	41.0
32	36.172574829126404	38.0	35.0	41.0	30.0	41.0
33	35.93251618670838	38.0	35.0	41.0	29.0	41.0
34	35.68109432489501	38.0	35.0	41.0	27.0	41.0
35	35.44793150378774	38.0	35.0	41.0	26.0	41.0
36	35.28735535370585	38.0	34.0	40.0	25.0	41.0
37	35.2045479563551	38.0	34.0	40.0	25.0	41.0
38	35.10274515232685	38.0	34.0	40.0	25.0	41.0
39	34.97534299810196	38.0	34.0	40.0	24.0	41.0
40	34.869054449385054	38.0	34.0	40.0	23.0	41.0
41	34.803065050386444	38.0	34.0	40.0	23.0	41.0
42	34.7756126931814	38.0	34.0	40.0	23.0	41.0
43	34.68930359626797	38.0	34.0	40.0	23.0	41.0
44	34.57412278214575	37.0	34.0	40.0	23.0	41.0
45	34.49239449868738	37.0	34.0	40.0	23.0	41.0
46	34.4400249513815	37.0	34.0	40.0	23.0	41.0
47	34.38412618460686	37.0	33.0	40.0	23.0	41.0
48	34.26174432839754	37.0	33.0	40.0	23.0	41.0
49	34.18770077022379	37.0	33.0	40.0	23.0	41.0
50	34.06720361061168	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	3.0
13	8.0
14	14.0
15	41.0
16	93.0
17	229.0
18	539.0
19	987.0
20	1743.0
21	2884.0
22	4254.0
23	6155.0
24	8869.0
25	13417.0
26	19302.0
27	24761.0
28	26563.0
29	27151.0
30	29240.0
31	33870.0
32	41074.0
33	51860.0
34	84998.0
35	122909.0
36	85533.0
37	113507.0
38	179030.0
39	320091.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.007208547516036	22.651801469729772	29.041339902529494	15.299650080224698
2	33.835724507393685	24.347194470667116	29.10355156896822	12.713529452970981
3	28.82718499714794	24.568604624011368	33.69220402757995	12.912006351260747
4	25.32131575172708	26.960835003986215	33.42684541818582	14.291003826100878
5	22.616108985499512	31.929929315538242	31.81684751970592	13.63711417925633
6	21.56726698978928	40.18590113515443	28.134350513538127	10.112481361518165
7	85.85560221893836	3.566746613383681	8.853153781235093	1.72449738644286
8	86.62440832201959	2.8599019957769456	8.94021675678741	1.575472925416051
9	81.14244303379444	5.996420744338405	10.291110569978953	2.570025651888199
10	36.34979301694893	38.11456953863294	15.237438413785972	10.298199030632157
11	35.0878802333688	22.221406817598062	27.26405433263394	15.426658616399195
12	31.38136585463485	22.064376565715868	30.10435881954614	16.44989876010314
13	23.981012932687978	26.9831845034575	31.41047024014037	17.625332323714154
14	18.538576236811295	29.75235420287341	33.55377056070557	18.155298999609716
15	18.48520429777539	23.705647084724617	39.98417188432967	17.824976733170324
16	21.95796626226304	22.0567877431342	36.7636757254414	19.221570269161358
17	21.776501669540966	21.611215445839157	31.267867090528817	25.344415794091056
18	23.222547642794954	23.3131131518465	33.046737139864504	20.41760206549404
19	25.846779170266494	23.531521133619986	29.705653756217	20.916045939896524
20	27.76316535627437	23.85216973610912	29.352064660104144	19.03260024751237
21	25.046700446656416	24.115109929515683	30.871914017806212	19.96627560602169
22	24.408822381522636	22.391613266929745	30.980158981663404	22.219405369884214
23	21.153384281296805	25.238589246221434	30.91961518831955	22.68841128416221
24	21.849220936477384	23.85617263153681	34.39145982260502	19.903146609380784
25	20.967916793147044	25.12742550444822	32.410109979551876	21.494547722852865
26	20.48298269081302	28.10766454402017	29.702568190991485	21.70678457417532
27	20.37540487619378	27.02913440722122	32.147920329038	20.447540387546994
28	19.405036309597275	25.204314454121814	35.05869245420855	20.331956782072368
29	20.291427465866978	23.49391059533062	34.55674604630683	21.657915892495573
30	21.144794734858213	25.74570606071725	32.78504785127909	20.32445135314544
31	24.534746800185466	25.039862166967442	29.84083487055637	20.584556162290724
32	25.68799765163468	25.54998115303403	29.286100279201953	19.475920916129333
33	24.76799885250331	26.002892091946507	29.319207560135162	19.909901495415017
34	21.482956004843505	26.20879102550845	30.729894623777867	21.57835834587018
35	21.846218764906617	26.53677826961502	30.430177828629372	21.186825136848988
36	25.356174299409908	26.286347124419997	28.406880977240206	19.950597598929892
37	22.51595320615245	28.81826187609037	28.350089898359816	20.315695019397364
38	22.66422712428657	28.632460813321636	27.533582624765245	21.16972943762655
39	22.63187038624605	28.397957856182636	27.66734604697398	21.302825710597332
40	23.393838209638304	26.157587321495885	27.759996397394115	22.6885780714717
41	20.88552386226037	26.55462451173015	28.35367582551379	24.206175800495693
42	22.03610611675779	26.48574135291194	27.792436529089375	23.6857160012409
43	22.372849694612437	26.14808044485511	28.354509762061227	23.12456009847123
44	21.92227377803278	27.38689318607126	29.133072922747456	21.55776011314851
45	20.767021478869715	29.326379414443117	27.459195484733957	22.447403621953214
46	21.643655577534414	28.622036606478684	28.399125367349047	21.33518244863785
47	21.812194153771227	27.23303189306932	28.979211629745517	21.975562323413936
48	22.384358018967053	25.627120283671857	30.45186017886271	21.53666151849838
49	22.185047184129854	25.36876674127619	30.636660517774523	21.809525556819434
50	20.286757421201333	28.93926606912333	29.33563611011965	21.438340399555678
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	346.0
1	1190.0
2	2034.0
3	11322.0
4	20610.0
5	14623.5
6	8637.0
7	8467.0
8	8297.0
9	8199.5
10	8102.0
11	7718.5
12	7335.0
13	6915.5
14	6496.0
15	6104.5
16	5713.0
17	5613.5
18	5514.0
19	5144.0
20	4774.0
21	5158.0
22	5542.0
23	5988.0
24	6434.0
25	7600.0
26	8766.0
27	10482.0
28	12198.0
29	15944.5
30	19691.0
31	21780.5
32	23870.0
33	27584.0
34	31298.0
35	34211.0
36	37124.0
37	40997.0
38	44870.0
39	51230.0
40	57590.0
41	67487.5
42	77385.0
43	83458.5
44	89532.0
45	90461.0
46	91390.0
47	89948.0
48	88506.0
49	86670.0
50	84834.0
51	81633.5
52	78433.0
53	73213.5
54	67994.0
55	64402.5
56	60811.0
57	59028.5
58	57246.0
59	53259.5
60	49273.0
61	45545.0
62	41817.0
63	36730.5
64	31644.0
65	26012.0
66	20380.0
67	16879.0
68	13378.0
69	11087.5
70	8797.0
71	7256.5
72	5716.0
73	4574.0
74	3432.0
75	2537.0
76	1642.0
77	1217.0
78	792.0
79	608.0
80	424.0
81	338.5
82	253.0
83	190.0
84	127.0
85	84.0
86	41.0
87	30.0
88	19.0
89	18.0
90	17.0
91	9.0
92	1.0
93	2.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1199132.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.762782169088364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05473404477621	15.791078758996377
2	5.965912929021969	2.477379011700622
3	2.3172783802632964	1.4433943870363422
4	1.4303185775258802	1.1878957223028075
5	1.0774862520907484	1.1185806171173822
6	0.8407239098025758	1.0473460442145108
7	0.7323394090287556	1.064378253645207
8	0.6476711438625989	1.0757963901778749
9	0.5601645304782563	1.0467516712652727
>10	8.287829120361186	40.8383152787233
>50	1.884540313170125	26.78168148982895
>100	0.2001892627916493	5.075718661771979
>500	0.0	0.0
>1k	4.060634133704852E-4	0.11727529622319187
>5k	0.0	0.0
>10k+	4.060634133704852E-4	0.9344084169961433
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11083	0.9242518755232952	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1391	0.11600057374834465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0235170106376946	0.0
2	0.0	0.0	0.0	0.07722252429257162	0.0
3	0.0	0.0	0.0	0.11124713542795955	0.0
4	0.0	0.0	0.0	0.15169305797860452	0.0
5	0.0	0.0	0.0	0.25393367869425554	0.0
6	0.0	0.0	0.0	0.3550901818982397	0.0
7	0.0	0.0	0.0	0.42705890594196466	0.0
8	0.0	0.0	0.0	0.5997671649159558	0.0
9	0.0	0.0	0.0	0.6817431275289125	0.0
10	0.0	0.0	0.0	0.8199264134390543	0.0
11	0.0	0.0	0.0	0.9530226864098365	0.0
12	0.0	0.0	0.0	1.0724424000026687	0.0
13	0.0	0.0	0.0	1.1219782309203656	0.0
14	0.0	0.0	0.0	1.141825920749342	0.0
15	0.0	0.0	0.0	1.1710970935643448	0.0
16	0.0	0.0	0.0	1.2332253663483252	0.0
17	0.0	0.0	0.0	1.3123659446999996	0.0
18	0.0	0.0	0.0	1.4221953879973182	0.0
19	0.0	0.0	0.0	1.481071308246298	0.0
20	0.0	0.0	0.0	1.5486201685886125	0.0
21	0.0	0.0	0.0	1.6284278961782357	0.0
22	0.0	0.0	0.0	1.7221623641100396	0.0
23	0.0	0.0	0.0	1.8182318543746643	0.0
24	0.0	0.0	0.0	1.8919518451680049	0.0
25	0.0	0.0	0.0	1.9585833753081396	0.0
26	0.0	0.0	0.0	2.025715267376736	0.0
27	0.0	0.0	0.0	2.0908457117314856	0.0
28	0.0	0.0	0.0	2.164065340596365	0.0
29	0.0	0.0	0.0	2.2475423889947064	0.0
30	0.0	0.0	0.0	2.3522014256979213	0.0
31	0.0	0.0	0.0	2.446519649212931	0.0
32	0.0	0.0	0.0	2.5332490501462726	0.0
33	0.0	0.0	0.0	2.619144514532178	0.0
34	0.0	0.0	0.0	2.721385135247829	0.0
35	0.0	0.0	0.0	2.832382089711558	0.0
36	0.0	0.0	0.0	2.9288685482499006	0.0
37	0.0	0.0	0.0	3.0290243275969617	0.0
38	0.0	0.0	0.0	3.1549487462597945	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGATC	20	7.8566186E-4	44.0	31
TTCACGC	20	7.8566186E-4	44.0	20
TACGCAC	30	2.5278077E-6	44.0	2
TCATCGT	25	4.4429562E-5	44.0	17
CGCCCAT	25	4.4429562E-5	44.0	43
CGTTTTT	6905	0.0	42.853004	1
CGGTCTA	145	0.0	42.48276	31
CGTTTGG	105	0.0	39.80952	2
CGTTAGG	150	0.0	39.6	2
ATTAGCG	85	0.0	38.823532	1
CGCATGG	80	0.0	38.5	2
CTCGCGA	35	7.290473E-6	37.714283	14
TAGGGAT	1995	0.0	37.493732	5
ATGGGCG	360	0.0	37.27778	5
TACGGGA	580	0.0	37.172417	4
TATAGCG	95	0.0	37.05263	1
TAAGGGA	1925	0.0	36.914288	4
CGAGGGA	830	0.0	36.843376	4
AGGGATT	2325	0.0	36.71398	6
TATACGG	60	1.9826984E-10	36.666664	2
>>END_MODULE