FastQCFastQC Report
Thu 26 May 2016
SRR1545871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545871_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences75700
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.83223249669749No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA1010.1334214002642008No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA760.10039630118890357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGG207.8099116E-444.0000043
GTATTAG207.8099116E-444.0000041
CACGGGG207.8099116E-444.0000044
GGAGAGT207.8099116E-444.0000048
TTATAGG207.8099116E-444.0000042
CGGGGTC207.8099116E-444.0000046
GGCTTAG207.8099116E-444.0000041
TACGCGG207.8099116E-444.0000042
ACATGCA800.044.00000444
TTCGGGG207.8099116E-444.0000044
GGGATTG408.13634E-944.0000047
GTCGGGG207.8099116E-444.0000044
AGTCGGG207.8099116E-444.0000043
GTACGCG207.8099116E-444.0000041
TGCTTGG254.4034285E-544.02
GTAGGGA850.044.04
ACGGGAT254.4034285E-544.05
GCCGATG254.4034285E-544.09
GTAGAAG254.4034285E-544.01
CTTGCGG254.4034285E-544.02
Produced by FastQC (version 0.11.5)