##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545871_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 75700 Sequences flagged as poor quality 0 Sequence length 50 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.540660501981506 31.0 31.0 33.0 30.0 34.0 2 31.956723910171732 31.0 31.0 34.0 30.0 34.0 3 32.05550858652576 33.0 31.0 34.0 30.0 34.0 4 35.790964332893 37.0 35.0 37.0 35.0 37.0 5 35.727093791281376 37.0 35.0 37.0 33.0 37.0 6 35.76988110964333 37.0 35.0 37.0 33.0 37.0 7 36.003262879788636 37.0 35.0 37.0 35.0 37.0 8 35.981545574636726 37.0 35.0 37.0 35.0 37.0 9 37.76844121532365 39.0 38.0 39.0 35.0 39.0 10 37.3378071334214 39.0 37.0 39.0 34.0 39.0 11 37.07284015852048 39.0 37.0 39.0 33.0 39.0 12 36.52344782034346 39.0 35.0 39.0 33.0 39.0 13 36.27129458388375 38.0 35.0 39.0 32.0 39.0 14 37.34182298546896 39.0 35.0 41.0 32.0 41.0 15 37.48772787318362 39.0 35.0 41.0 33.0 41.0 16 37.630211360634085 40.0 35.0 41.0 33.0 41.0 17 37.52273447820343 39.0 35.0 41.0 33.0 41.0 18 37.42175693527081 39.0 35.0 41.0 33.0 41.0 19 37.34550858652576 39.0 35.0 41.0 33.0 41.0 20 37.167199471598416 39.0 35.0 41.0 32.0 41.0 21 37.06648612945839 39.0 35.0 41.0 32.0 41.0 22 36.954253632760896 38.0 35.0 41.0 32.0 41.0 23 36.85273447820344 38.0 35.0 41.0 32.0 41.0 24 36.89869220607662 38.0 35.0 41.0 32.0 41.0 25 36.886393659180975 38.0 35.0 41.0 32.0 41.0 26 36.61842800528402 38.0 35.0 41.0 31.0 41.0 27 36.635085865257594 38.0 35.0 41.0 31.0 41.0 28 36.636235138705416 38.0 35.0 41.0 31.0 41.0 29 36.61392338177014 38.0 35.0 41.0 31.0 41.0 30 36.514478203434614 38.0 35.0 41.0 31.0 41.0 31 36.37747688243065 38.0 35.0 41.0 31.0 41.0 32 36.142892998678995 38.0 35.0 41.0 30.0 41.0 33 35.91799207397622 38.0 35.0 41.0 30.0 41.0 34 35.68733157199472 38.0 35.0 41.0 29.0 41.0 35 35.462457067371204 38.0 35.0 41.0 27.0 41.0 36 35.32110964332893 38.0 35.0 40.0 26.0 41.0 37 35.2060105680317 38.0 34.0 40.0 26.0 41.0 38 35.13182298546896 38.0 34.0 40.0 26.0 41.0 39 35.04647291941876 38.0 34.0 40.0 25.0 41.0 40 34.88836195508586 38.0 34.0 40.0 24.0 41.0 41 34.8678071334214 38.0 34.0 40.0 24.0 41.0 42 34.853051519154555 38.0 34.0 40.0 24.0 41.0 43 34.75079260237781 37.0 34.0 40.0 24.0 41.0 44 34.672483487450464 37.0 34.0 40.0 24.0 41.0 45 34.57849405548217 37.0 34.0 40.0 23.0 41.0 46 34.522892998679 37.0 34.0 40.0 23.0 41.0 47 34.44647291941876 37.0 34.0 40.0 23.0 41.0 48 34.347833553500664 36.0 34.0 40.0 23.0 41.0 49 34.26750330250991 36.0 34.0 40.0 24.0 41.0 50 34.1505680317041 36.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.0 15 2.0 16 2.0 17 20.0 18 29.0 19 48.0 20 89.0 21 152.0 22 238.0 23 364.0 24 507.0 25 773.0 26 1164.0 27 1486.0 28 1620.0 29 1610.0 30 1835.0 31 2137.0 32 2534.0 33 3326.0 34 5775.0 35 9465.0 36 5124.0 37 6663.0 38 10679.0 39 20055.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.583883751651253 23.044914134742402 28.723910171730516 16.647291941875825 2 32.015852047556145 25.257595772787315 30.079260237780716 12.647291941875826 3 28.21796565389696 24.756935270805812 34.34874504623514 12.676354029062086 4 25.875825627476885 27.524438573315717 32.949801849405546 13.649933949801849 5 23.009247027741083 32.01188903566711 31.54293262879789 13.435931307793926 6 21.766182298546894 39.99075297225892 27.76486129458388 10.478203434610304 7 85.5442536327609 4.002642007926024 8.787318361955085 1.6657859973579923 8 87.17437252311757 2.906208718626156 8.310435931307794 1.608982826948481 9 81.90885072655219 6.03434610303831 9.65125495376486 2.40554821664465 10 40.30118890356671 36.41347424042272 13.924702774108322 9.360634081902246 11 40.47027741083223 19.937912813738443 25.51651254953765 14.075297225891678 12 37.38177014531043 20.960369881109646 26.330250990752972 15.327608982826948 13 22.262879788639367 32.626155878467635 28.848084544253634 16.262879788639367 14 16.631439894319684 34.44253632760898 30.834874504623517 18.091149273447822 15 15.93659180977543 24.53764861294584 43.657859973579924 15.867899603698813 16 19.642007926023776 20.49801849405548 41.45310435931307 18.40686922060766 17 19.002642007926024 21.136063408190225 30.815059445178335 29.046235138705416 18 21.31571994715984 24.128137384412156 33.376486129458385 21.179656538969617 19 26.72787318361955 23.550858652575958 29.009247027741083 20.712021136063406 20 28.240422721268168 23.577278731836195 28.74768824306473 19.43461030383091 21 24.1889035667107 25.55350066050198 29.558784676354026 20.69881109643329 22 23.877146631439892 23.38705416116248 28.69749009247028 24.038309114927344 23 20.994715984147952 27.249669749009247 28.776750330250987 22.978863936591807 24 21.03566710700132 23.480845442536328 36.408190224570674 19.075297225891678 25 19.795244385733156 24.655217965653897 34.04359313077939 21.50594451783355 26 19.626155878467635 30.228533685601057 29.734478203434612 20.4108322324967 27 20.120211360634084 29.550858652575958 30.76750330250991 19.561426684280054 28 17.752972258916778 26.81902245706737 36.65125495376486 18.77675033025099 29 18.52443857331572 24.32760898282695 35.908850726552174 21.239101717305154 30 20.044914134742402 26.9445178335535 32.57199471598415 20.438573315719946 31 26.03038309114927 25.457067371202115 28.110964332893 20.401585204755616 32 27.36327608982827 25.981505944517835 28.487450462351386 18.16776750330251 33 25.660501981505945 27.009247027741086 27.916776750330254 19.41347424042272 34 21.77675033025099 26.150594451783356 30.498018494055483 21.57463672391017 35 21.41875825627477 25.449141347424042 31.383091149273447 21.749009247027743 36 27.34081902245707 26.005284015852048 27.599735799207398 19.05416116248349 37 22.066050198150595 29.605019815059446 29.221928665785995 19.107001321003963 38 22.09643328929987 29.533685601056803 26.845442536327607 21.52443857331572 39 22.58124174372523 29.158520475561428 28.01321003963012 20.247027741083222 40 23.511228533685603 26.430647291941877 28.01188903566711 22.046235138705416 41 19.364597093791282 26.78996036988111 29.043593130779392 24.801849405548214 42 22.40554821664465 26.682959048877148 26.210039630118892 24.701453104359313 43 22.898282694848085 27.730515191545575 26.990752972258917 22.380449141347427 44 20.751651254953764 29.394980184940557 28.2668428005284 21.58652575957728 45 19.67107001321004 32.28533685601057 25.885072655217968 22.158520475561428 46 21.331571994715983 30.857331571994717 27.886393659180982 19.924702774108322 47 22.55350066050198 27.270805812417436 28.200792602377806 21.974900924702776 48 22.885072655217968 24.834874504623514 29.891677675033023 22.388375165125495 49 20.842800528401586 25.409511228533688 31.554821664464995 22.192866578599734 50 20.393659180977544 29.618229854689567 28.499339498018493 21.4887714663144 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22.0 1 66.5 2 111.0 3 669.0 4 1227.0 5 864.0 6 501.0 7 504.5 8 508.0 9 495.5 10 483.0 11 501.0 12 519.0 13 469.5 14 420.0 15 374.0 16 328.0 17 313.5 18 299.0 19 291.0 20 283.0 21 298.5 22 314.0 23 346.0 24 378.0 25 442.0 26 506.0 27 667.5 28 829.0 29 1008.0 30 1187.0 31 1374.5 32 1562.0 33 1851.5 34 2141.0 35 2330.0 36 2519.0 37 2610.5 38 2702.0 39 3133.5 40 3565.0 41 4317.5 42 5070.0 43 5711.0 44 6352.0 45 6300.0 46 6248.0 47 6192.5 48 6137.0 49 5716.0 50 5295.0 51 5043.5 52 4792.0 53 4565.0 54 4338.0 55 3958.5 56 3579.0 57 3389.0 58 3199.0 59 3133.0 60 3067.0 61 2867.0 62 2667.0 63 2178.5 64 1690.0 65 1390.5 66 1091.0 67 883.0 68 675.0 69 554.5 70 434.0 71 367.5 72 301.0 73 226.0 74 151.0 75 121.0 76 91.0 77 83.0 78 75.0 79 52.0 80 29.0 81 17.0 82 5.0 83 5.0 84 5.0 85 4.0 86 3.0 87 2.0 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 75700.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.42140026420079 #Duplication Level Percentage of deduplicated Percentage of total 1 61.86591100177499 26.2443857331572 2 11.898608040357487 10.095112285336857 3 7.12484040731168 9.067371202113607 4 5.026001930682279 8.528401585204756 5 3.7772864571980196 8.011889035667107 6 2.65001712702021 6.745046235138706 7 2.2233986236103758 6.602377807133421 8 1.6722199732195682 5.675033025099075 9 1.1646373742721017 4.446499339498018 >10 2.5815090461806744 13.376486129458387 >50 0.009342011023573007 0.2417437252311757 >100 0.003114003674524336 0.1334214002642008 >500 0.003114003674524336 0.83223249669749 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 630 0.83223249669749 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 101 0.1334214002642008 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 76 0.10039630118890357 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.019815059445178335 0.0 2 0.0 0.0 0.0 0.08718626155878467 0.0 3 0.0 0.0 0.0 0.12945838837516513 0.0 4 0.0 0.0 0.0 0.18758256274768825 0.0 5 0.0 0.0 0.0 0.33157199471598414 0.0 6 0.0 0.0 0.0 0.45442536327608984 0.0 7 0.0 0.0 0.0 0.5442536327608983 0.0 8 0.0 0.0 0.0 0.7767503302509907 0.0 9 0.0 0.0 0.0 0.8758256274768824 0.0 10 0.0 0.0 0.0 1.0805812417437253 0.0 11 0.0 0.0 0.0 1.2113606340819023 0.0 12 0.0 0.0 0.0 1.35667107001321 0.0 13 0.0 0.0 0.0 1.4121532364597094 0.0 14 0.0 0.0 0.0 1.4359313077939233 0.0 15 0.0 0.0 0.0 1.464993394980185 0.0 16 0.0 0.0 0.0 1.5442536327608982 0.0 17 0.0 0.0 0.0 1.6446499339498017 0.0 18 0.0 0.0 0.0 1.7899603698811097 0.0 19 0.0 0.0 0.0 1.8428005284015851 0.0 20 0.0 0.0 0.0 1.9088507265521797 0.0 21 0.0 0.0 0.0 1.9735799207397622 0.0 22 0.0 0.0 0.0 2.0739762219286657 0.0 23 0.0 0.0 0.0 2.1611624834874505 0.0 24 0.0 0.0 0.0 2.214002642007926 0.0 25 0.0 0.0 0.0 2.2655217965653898 0.0 26 0.0 0.0 0.0 2.3289299867899604 0.0 27 0.0 0.0 0.0 2.3844121532364597 0.0 28 0.0 0.0 0.0 2.461030383091149 0.0 29 0.0 0.0 0.0 2.532364597093791 0.0 30 0.0 0.0 0.0 2.603698811096433 0.0 31 0.0 0.0 0.0 2.673712021136063 0.0 32 0.0 0.0 0.0 2.751651254953765 0.0 33 0.0 0.0 0.0 2.833553500660502 0.0 34 0.0 0.0 0.0 2.906208718626156 0.0 35 0.0 0.0 0.0 3.0079260237780714 0.0 36 0.0 0.0 0.0 3.092470277410832 0.0 37 0.0 0.0 0.0 3.169088507265522 0.0 38 0.0 0.0 0.0 3.260237780713342 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACAGGG 20 7.8099116E-4 44.000004 3 GTATTAG 20 7.8099116E-4 44.000004 1 CACGGGG 20 7.8099116E-4 44.000004 4 GGAGAGT 20 7.8099116E-4 44.000004 8 TTATAGG 20 7.8099116E-4 44.000004 2 CGGGGTC 20 7.8099116E-4 44.000004 6 GGCTTAG 20 7.8099116E-4 44.000004 1 TACGCGG 20 7.8099116E-4 44.000004 2 ACATGCA 80 0.0 44.000004 44 TTCGGGG 20 7.8099116E-4 44.000004 4 GGGATTG 40 8.13634E-9 44.000004 7 GTCGGGG 20 7.8099116E-4 44.000004 4 AGTCGGG 20 7.8099116E-4 44.000004 3 GTACGCG 20 7.8099116E-4 44.000004 1 TGCTTGG 25 4.4034285E-5 44.0 2 GTAGGGA 85 0.0 44.0 4 ACGGGAT 25 4.4034285E-5 44.0 5 GCCGATG 25 4.4034285E-5 44.0 9 GTAGAAG 25 4.4034285E-5 44.0 1 CTTGCGG 25 4.4034285E-5 44.0 2 >>END_MODULE