FastQCFastQC Report
Thu 26 May 2016
SRR1545870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545870_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4409959
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT514121.1658158273126802No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA61640.13977454212159343No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA56280.12762023411102008No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA44290.10043177272169651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG1300.044.0000041
CGTTTTT307400.042.5400121
TAGGGCG15050.038.445185
ATTACGG3150.038.4126972
CACGACG7750.038.32258226
CGACGGT7950.038.1886828
GGCGATA17200.038.116288
GACCGAT17300.038.023129
AATACGG3300.038.02
CGAAAGG5800.037.9310342
TATGGGC13350.037.737834
AACGGGA17450.037.4441264
GGGCGAT67050.037.404927
ACGGGAC12150.037.300415
CATACGA13300.037.21804418
TACGGGA20600.036.7378654
AAGGGAT64250.036.6723755
GAACGGG15900.036.6666683
TACGCTA301.3016943E-436.66666424
GTCGTAC301.3016943E-436.66666419
Produced by FastQC (version 0.11.5)