##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545870_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4409959
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.515646290589096	31.0	31.0	33.0	30.0	34.0
2	31.927618828202256	31.0	31.0	34.0	30.0	34.0
3	31.99306410785225	33.0	31.0	34.0	30.0	34.0
4	35.760476004425435	37.0	35.0	37.0	35.0	37.0
5	35.69002795717602	37.0	35.0	37.0	33.0	37.0
6	35.7295251497803	37.0	35.0	37.0	33.0	37.0
7	35.98007532496334	37.0	35.0	37.0	35.0	37.0
8	35.95540933600517	37.0	35.0	37.0	35.0	37.0
9	37.70970546438187	39.0	38.0	39.0	35.0	39.0
10	37.24629458006299	39.0	37.0	39.0	34.0	39.0
11	37.044537148758074	39.0	37.0	39.0	33.0	39.0
12	36.6214744853637	39.0	35.0	39.0	33.0	39.0
13	36.451676988380164	39.0	35.0	39.0	32.0	39.0
14	37.5901288424677	40.0	36.0	41.0	33.0	41.0
15	37.66498033201669	40.0	35.0	41.0	33.0	41.0
16	37.73863430476338	40.0	35.0	41.0	33.0	41.0
17	37.61968217845109	40.0	35.0	41.0	33.0	41.0
18	37.50659291843756	39.0	36.0	41.0	32.0	41.0
19	37.38000081179893	39.0	36.0	41.0	32.0	41.0
20	37.20273884632488	39.0	35.0	41.0	32.0	41.0
21	37.08408128057427	39.0	35.0	41.0	32.0	41.0
22	36.98699534394764	39.0	35.0	41.0	32.0	41.0
23	36.872652784300264	38.0	35.0	41.0	32.0	41.0
24	36.922387260289725	39.0	35.0	41.0	32.0	41.0
25	36.907968532133744	38.0	35.0	41.0	32.0	41.0
26	36.689014115550734	38.0	35.0	41.0	31.0	41.0
27	36.69473344310004	38.0	35.0	41.0	31.0	41.0
28	36.65783831550361	38.0	35.0	41.0	31.0	41.0
29	36.587266684338786	38.0	35.0	41.0	31.0	41.0
30	36.443915918492664	38.0	35.0	41.0	31.0	41.0
31	36.2976757833803	38.0	35.0	41.0	30.0	41.0
32	36.09705804521085	38.0	35.0	41.0	30.0	41.0
33	35.82169902259862	38.0	35.0	41.0	29.0	41.0
34	35.524141380906265	38.0	35.0	41.0	27.0	41.0
35	35.28155976053292	38.0	34.0	41.0	25.0	41.0
36	35.11459789081939	38.0	34.0	40.0	24.0	41.0
37	35.04491424976967	38.0	34.0	40.0	24.0	41.0
38	34.95065350947707	38.0	34.0	40.0	24.0	41.0
39	34.82223394820677	38.0	34.0	40.0	23.0	41.0
40	34.71525132093065	38.0	34.0	40.0	23.0	41.0
41	34.66421978072812	38.0	34.0	40.0	23.0	41.0
42	34.62467451511454	38.0	34.0	40.0	23.0	41.0
43	34.528072029694606	38.0	34.0	40.0	23.0	41.0
44	34.410233519177844	37.0	34.0	40.0	23.0	41.0
45	34.322356965223484	37.0	34.0	40.0	23.0	41.0
46	34.24074509536256	37.0	33.0	40.0	22.0	41.0
47	34.186708085041154	37.0	33.0	40.0	22.0	41.0
48	34.058821862062665	37.0	33.0	40.0	22.0	41.0
49	33.99298088712389	36.0	33.0	40.0	22.0	41.0
50	33.87917688123631	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	10.0
12	16.0
13	24.0
14	47.0
15	126.0
16	348.0
17	821.0
18	1799.0
19	3560.0
20	6332.0
21	10287.0
22	15686.0
23	23331.0
24	34348.0
25	51520.0
26	76341.0
27	97016.0
28	102596.0
29	103265.0
30	110150.0
31	123680.0
32	148378.0
33	187637.0
34	313488.0
35	469398.0
36	307923.0
37	397959.0
38	630751.0
39	1193117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.292259633252826	22.794293552389036	26.9831760340629	16.93027078029524
2	35.01397632041477	23.906707522677646	28.682352829130608	12.396963327776971
3	28.531829887760864	24.077162622146826	34.36440112028252	13.026606369809787
4	25.972259606041685	26.124573947286134	33.641514580974565	14.261651865697617
5	23.077606843963856	31.92630589082574	31.48589363302471	13.510193632185697
6	21.476707606578653	39.74522212111269	28.374232050683464	10.40383822162519
7	83.56093560053506	4.06849587490496	10.518555841448867	1.852012683111113
8	84.47182842289463	3.2927517013196717	10.430550488111114	1.8048693876745792
9	78.3453088792889	6.724234851163015	11.821810588261705	3.1086456812863794
10	36.22850008356087	35.786319101832916	17.05061203516858	10.934568779437633
11	35.02996286360032	21.96984144297033	27.58993904478477	15.41025664864458
12	32.38132145899769	21.40264796112617	29.902273467848566	16.31375711202757
13	22.448122533565503	28.38146567802558	30.840490807284148	18.32992098112477
14	17.727443724533494	30.83534336713788	32.55247951284807	18.884733395480545
15	18.208876771870212	24.397845875664604	40.38268836512992	17.010588987335257
16	21.066272951743994	21.34994905848331	38.248927030840875	19.334850958931817
17	19.737144948513127	22.555039627352542	32.3021597252945	25.40565569883983
18	22.26587594125025	23.561715653138727	33.76868129612996	20.40372710948106
19	25.27225309804468	23.98804614736781	30.058805535380262	20.680895219207255
20	27.67055203914594	22.649621005546763	30.28669881057851	19.393128144728784
21	23.448902812928647	25.475588321796188	31.09992632584566	19.975582539429503
22	23.404752742599193	22.847219214509703	30.952033794418497	22.795994248472603
23	21.836960388974138	26.204143848049384	30.788585562813626	21.17031020016286
24	20.981215471617766	23.399559950557364	35.89890518256519	19.720319395259683
25	20.045787273759235	25.234542996885008	33.14747370667165	21.57219602268411
26	19.88331410790894	28.736639048118136	30.30867180397822	21.071375039994702
27	19.686305473588302	28.15908265813809	31.382854126308203	20.771757741965402
28	18.2854307715786	26.785895288368895	35.13252617541342	19.796147764639084
29	20.111139355263845	23.96437245788453	34.19083941596736	21.733648770884265
30	20.24615194835145	26.32110638670337	33.14414034234785	20.28860132259733
31	24.777849408577268	25.778675039835967	29.397597574036404	20.045877977550358
32	25.924186596746136	25.875161197643788	29.78927922005624	18.411372985553832
33	24.17573496715049	26.676325108691483	29.156234785856284	19.99170513830174
34	20.780238546435463	27.019820365676868	31.0290413130825	21.170899774805164
35	22.21478703089983	26.082940907160364	30.59046580705172	21.111806254888084
36	24.050495707556465	28.23361849849398	28.442282569974008	19.273603223975552
37	21.886983529778846	29.974156222314086	28.60922289753714	19.529637350369924
38	21.175571020048032	29.686171685496394	27.766176510938084	21.37208078351749
39	22.431841203058802	28.907162175430656	27.81658514285507	20.84441147865547
40	22.514653764354726	27.20356357054567	28.448427751822635	21.833354913276974
41	19.80546757917704	27.254312341679366	28.825551439367125	24.114668639776472
42	20.854797062739134	27.629916740722532	27.105217985019813	24.41006821151852
43	22.17206554528058	26.998482298815023	27.691572642738855	23.13787951316554
44	21.17872297678958	28.082097815421868	28.585707939688326	22.153471268100226
45	20.236514670544555	29.930822486104745	27.28587726099041	22.54678558236029
46	20.907632021068675	30.06451987422105	28.60094617659711	20.426901928113164
47	21.569180121629252	27.48463194329018	29.365692515508645	21.58049541957193
48	21.331853652154138	25.777269131073556	31.105300525469737	21.785576691302573
49	20.776474339103835	26.70154076262387	30.473276508919923	22.048708389352374
50	19.644944544835905	29.02473696467473	29.430137559102022	21.900180931387343
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1645.0
1	5287.5
2	8930.0
3	53453.5
4	97977.0
5	69049.5
6	40122.0
7	38030.5
8	35939.0
9	35176.5
10	34414.0
11	32521.0
12	30628.0
13	28950.5
14	27273.0
15	25194.0
16	23115.0
17	21723.5
18	20332.0
19	20018.0
20	19704.0
21	19254.0
22	18804.0
23	22797.0
24	26790.0
25	31286.0
26	35782.0
27	44931.0
28	54080.0
29	66889.0
30	79698.0
31	92017.5
32	104337.0
33	111783.0
34	119229.0
35	131103.0
36	142977.0
37	151647.5
38	160318.0
39	182064.0
40	203810.0
41	241168.0
42	278526.0
43	296911.5
44	315297.0
45	319279.5
46	323262.0
47	317298.0
48	311334.0
49	306715.5
50	302097.0
51	287354.0
52	272611.0
53	258630.0
54	244649.0
55	231474.0
56	218299.0
57	210139.5
58	201980.0
59	194649.0
60	187318.0
61	169793.5
62	152269.0
63	130501.5
64	108734.0
65	90623.0
66	72512.0
67	60116.0
68	47720.0
69	39665.0
70	31610.0
71	27786.5
72	23963.0
73	19328.5
74	14694.0
75	11347.5
76	8001.0
77	6139.0
78	4277.0
79	3396.5
80	2516.0
81	1953.5
82	1391.0
83	952.0
84	513.0
85	374.5
86	236.0
87	168.0
88	100.0
89	79.5
90	59.0
91	42.0
92	25.0
93	25.0
94	25.0
95	28.5
96	32.0
97	18.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4409959.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.734674581176854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10760782407822	12.604671405884075
2	7.537188427028372	2.3719041431260743
3	2.4301812441191246	1.147143331484818
4	1.219870584249679	0.7677706669727513
5	0.7814127800470904	0.6147637903806846
6	0.5469021095462661	0.5163196032881779
7	0.4090370958626229	0.4505245916519609
8	0.30077227650868327	0.37860431151231005
9	0.27085110556623704	0.38355786054330465
>10	3.277834141557606	12.60959767454045
>50	1.4006111173073468	15.931046101850463
>100	1.7029289836230395	48.81699893089622
>500	0.011724602380755764	1.2111526624056912
>1k	0.002638035535670047	0.7384385399279344
>5k	2.9311505951889414E-4	0.27192765170289807
>10k+	1.4655752975944707E-4	1.185578733832208
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51412	1.1658158273126802	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	6164	0.13977454212159343	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5628	0.12762023411102008	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4429	0.10043177272169651	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023288198371005264	0.0
2	0.0	0.0	0.0	0.08619127751527848	0.0
3	0.0	0.0	0.0	0.12612362155747933	0.0
4	0.0	0.0	0.0	0.18292687074868497	0.0
5	0.0	0.0	0.0	0.326216184776321	0.0
6	0.0	0.0	0.0	0.4569203477855463	0.0
7	0.0	0.0	0.0	0.5477148427003516	0.0
8	0.0	0.0	0.0	0.7753133305774498	0.0
9	0.0	0.0	0.0	0.8821397205733659	0.0
10	2.2675947780920412E-5	0.0	0.0	1.087515779625162	0.0
11	2.2675947780920412E-5	0.0	0.0	1.241077297997555	0.0
12	2.2675947780920412E-5	0.0	0.0	1.4002851273673973	0.0
13	2.2675947780920412E-5	0.0	0.0	1.4580634423131824	0.0
14	2.2675947780920412E-5	0.0	0.0	1.4806033344074174	0.0
15	2.2675947780920412E-5	0.0	0.0	1.517746536872565	0.0
16	2.2675947780920412E-5	0.0	0.0	1.5953663061266556	0.0
17	2.2675947780920412E-5	0.0	0.0	1.688995294514076	0.0
18	2.2675947780920412E-5	0.0	0.0	1.8202436802700432	9.750657545795778E-4
19	2.2675947780920412E-5	0.0	0.0	1.8843485846467054	9.750657545795778E-4
20	2.2675947780920412E-5	0.0	0.0	1.9615375108929585	9.750657545795778E-4
21	2.2675947780920412E-5	0.0	0.0	2.054554248690294	9.750657545795778E-4
22	2.2675947780920412E-5	0.0	0.0	2.15768445919792	9.750657545795778E-4
23	2.2675947780920412E-5	0.0	0.0	2.2663249250163098	9.750657545795778E-4
24	2.2675947780920412E-5	0.0	0.0	2.3464163725785205	9.750657545795778E-4
25	2.2675947780920412E-5	0.0	0.0	2.4165530790649075	9.750657545795778E-4
26	2.2675947780920412E-5	0.0	0.0	2.4885492132693297	9.750657545795778E-4
27	2.2675947780920412E-5	0.0	0.0	2.5573026869410804	9.750657545795778E-4
28	2.2675947780920412E-5	0.0	0.0	2.6358975219497505	9.750657545795778E-4
29	2.2675947780920412E-5	0.0	0.0	2.7179391010211207	9.750657545795778E-4
30	2.2675947780920412E-5	0.0	0.0	2.8273959009596235	9.750657545795778E-4
31	2.2675947780920412E-5	0.0	0.0	2.9256507826943516	9.750657545795778E-4
32	2.2675947780920412E-5	0.0	0.0	3.0164906295047187	9.750657545795778E-4
33	2.2675947780920412E-5	0.0	0.0	3.1085776534430365	9.750657545795778E-4
34	2.2675947780920412E-5	0.0	0.0	3.210143223553779	9.750657545795778E-4
35	2.2675947780920412E-5	0.0	0.0	3.3286023747613074	9.750657545795778E-4
36	2.2675947780920412E-5	0.0	0.0	3.439850574574503	9.750657545795778E-4
37	2.2675947780920412E-5	0.0	0.0	3.5444320457401077	9.750657545795778E-4
38	2.2675947780920412E-5	0.0	0.0	3.6628004931565123	9.750657545795778E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAACG	130	0.0	44.000004	1
CGTTTTT	30740	0.0	42.540012	1
TAGGGCG	1505	0.0	38.44518	5
ATTACGG	315	0.0	38.412697	2
CACGACG	775	0.0	38.322582	26
CGACGGT	795	0.0	38.18868	28
GGCGATA	1720	0.0	38.11628	8
GACCGAT	1730	0.0	38.02312	9
AATACGG	330	0.0	38.0	2
CGAAAGG	580	0.0	37.931034	2
TATGGGC	1335	0.0	37.73783	4
AACGGGA	1745	0.0	37.444126	4
GGGCGAT	6705	0.0	37.40492	7
ACGGGAC	1215	0.0	37.30041	5
CATACGA	1330	0.0	37.218044	18
TACGGGA	2060	0.0	36.737865	4
AAGGGAT	6425	0.0	36.672375	5
GAACGGG	1590	0.0	36.666668	3
TACGCTA	30	1.3016943E-4	36.666664	24
GTCGTAC	30	1.3016943E-4	36.666664	19
>>END_MODULE