FastQCFastQC Report
Thu 26 May 2016
SRR1545869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545869_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1794475
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195931.0918513771437328No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42700.237952604522214No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.8576646E-444.01
GTCGATC207.8576646E-444.040
CTCCGTA302.5285153E-644.018
CTAACGT207.8576646E-444.030
TACGCCC254.4438424E-544.014
CGTACCC207.8576646E-444.014
TTACGCG302.5285153E-644.01
CGACTCA302.5285153E-644.018
GCGCTAA302.5285153E-644.022
TTCGATA254.4438424E-544.015
CGTTTTT141650.043.207911
CGACGGT2750.043.228
GCGATAT1550.041.161299
CACGACG2850.040.91227726
GCCGACC700.040.85714314
TGTAGCG2050.040.7804871
GGCGATA7250.040.662078
TACGTAG650.040.6153871
ACGAACG650.040.61538720
TCACGAC3050.040.39344425
Produced by FastQC (version 0.11.5)