##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545869_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1794475
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.537669792000447	31.0	31.0	33.0	30.0	34.0
2	31.935243455606795	31.0	31.0	34.0	30.0	34.0
3	31.97381796904387	33.0	31.0	34.0	30.0	34.0
4	35.7431103804734	37.0	35.0	37.0	35.0	37.0
5	35.67460845093969	37.0	35.0	37.0	33.0	37.0
6	35.73649953328968	37.0	35.0	37.0	33.0	37.0
7	36.03062511319467	37.0	35.0	37.0	35.0	37.0
8	36.02162470917678	37.0	35.0	37.0	35.0	37.0
9	37.82409339779044	39.0	38.0	39.0	35.0	39.0
10	37.32820462809457	39.0	37.0	39.0	34.0	39.0
11	37.047529778904696	39.0	37.0	39.0	33.0	39.0
12	36.44616503434152	38.0	35.0	39.0	33.0	39.0
13	36.20778835035317	38.0	35.0	39.0	32.0	39.0
14	37.26737848117137	39.0	35.0	41.0	32.0	41.0
15	37.43397483943772	39.0	35.0	41.0	33.0	41.0
16	37.55307262569833	39.0	35.0	41.0	33.0	41.0
17	37.50092032488611	39.0	35.0	41.0	33.0	41.0
18	37.36028922108137	39.0	35.0	41.0	33.0	41.0
19	37.20654843338581	38.0	35.0	41.0	32.0	41.0
20	36.99482745649842	38.0	35.0	41.0	32.0	41.0
21	36.870198247398264	38.0	35.0	41.0	32.0	41.0
22	36.743492107719526	38.0	35.0	41.0	32.0	41.0
23	36.68308335306984	38.0	35.0	40.0	32.0	41.0
24	36.715558032293565	38.0	35.0	41.0	32.0	41.0
25	36.70213906574346	38.0	35.0	41.0	32.0	41.0
26	36.46654926928489	38.0	35.0	41.0	31.0	41.0
27	36.46708591649368	38.0	35.0	41.0	31.0	41.0
28	36.43415706543696	38.0	35.0	40.0	31.0	41.0
29	36.408357876259075	38.0	35.0	40.0	31.0	41.0
30	36.294138954290254	38.0	35.0	40.0	31.0	41.0
31	36.09822259992477	38.0	35.0	40.0	30.0	41.0
32	35.83772858356901	38.0	35.0	40.0	30.0	41.0
33	35.526868304100084	38.0	35.0	40.0	28.0	41.0
34	35.23497011660792	38.0	35.0	40.0	26.0	41.0
35	34.967438944538095	38.0	34.0	40.0	24.0	41.0
36	34.766517226486854	38.0	34.0	40.0	23.0	41.0
37	34.68999623845414	38.0	34.0	40.0	23.0	41.0
38	34.61315760877137	38.0	34.0	40.0	23.0	41.0
39	34.50418534668914	37.0	34.0	40.0	23.0	41.0
40	34.3645874141462	37.0	34.0	40.0	23.0	41.0
41	34.33998021705513	37.0	34.0	40.0	22.0	41.0
42	34.30339514342635	37.0	34.0	40.0	22.0	41.0
43	34.20505050223603	37.0	33.0	40.0	22.0	41.0
44	34.08981122612463	37.0	33.0	40.0	21.0	41.0
45	33.99493612337871	37.0	33.0	40.0	21.0	41.0
46	33.941007258390336	36.0	33.0	40.0	21.0	41.0
47	33.90224215996322	36.0	33.0	40.0	20.0	41.0
48	33.80365176444364	36.0	33.0	40.0	20.0	41.0
49	33.74286462614414	36.0	33.0	40.0	20.0	41.0
50	33.58783822566489	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	9.0
14	12.0
15	37.0
16	91.0
17	277.0
18	719.0
19	1279.0
20	2433.0
21	4110.0
22	6256.0
23	9567.0
24	14883.0
25	23172.0
26	34616.0
27	42529.0
28	43389.0
29	41569.0
30	44471.0
31	50835.0
32	61829.0
33	80091.0
34	140491.0
35	234573.0
36	117821.0
37	150150.0
38	238742.0
39	450520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.825798631911837	23.28508338093314	27.975870379916127	16.913247607238883
2	33.63173072904331	25.333426211008792	29.067888936875686	11.966954123072208
3	26.60538597639978	24.571810696721883	35.78032572200783	13.042477604870506
4	24.376879031471603	25.64293177670349	36.441967706432244	13.538221485392663
5	21.686231349001797	31.36767020994998	33.891806795859516	13.054291645188703
6	20.10387439223171	39.496900207581604	30.305019573970103	10.094205826216582
7	84.5304058290029	3.5879017539949007	10.536897978517395	1.3447944384847936
8	85.88183173351537	2.5512754426782207	10.22477326237479	1.3421195614316164
9	81.03462015352679	5.536382507418605	11.420387578539684	2.008609760514914
10	40.425751264297354	34.97502054918569	15.984898089970603	8.614330096546343
11	37.7507627579097	20.323715850039704	28.636063472603407	13.289457919447193
12	34.79903592972875	20.90455425681606	29.795845581576785	14.500564231878405
13	21.397678986890316	33.459256885718666	30.15171568285989	14.99134844453113
14	15.284637568090945	35.41264158040652	33.40653951712896	15.896181334373564
15	15.281684058011397	23.142256091614538	46.535671993201355	15.04038785717271
16	16.86264784964962	20.477688460413212	43.93123336909124	18.72843032084593
17	17.846389612560777	20.4180052661642	32.26564872734365	29.469956393931373
18	20.938881845665165	22.28144721993898	36.54979868763844	20.22987224675741
19	24.65283718079104	23.35418437147355	31.62819208960838	20.36478635812703
20	27.119909722899454	22.629181236852002	30.305242480391204	19.94566655985734
21	21.740453335933907	25.439195307819833	32.65835411471322	20.16199724153304
22	21.93020242689366	22.8577717716881	31.753911311107707	23.458114490310535
23	19.244570138898563	26.299001100600456	31.22634753897379	23.230081221527186
24	18.460162443054376	23.24897254071525	40.7804511068697	17.510413909360675
25	17.07680519371961	25.338831691720422	36.95197759790468	20.632385516655287
26	17.470235026957745	30.337062372002954	32.197216456066535	19.995486144972762
27	17.679265523342483	29.69252845539782	33.56318700455565	19.06501901670405
28	15.585505509968097	26.29225818136224	39.803396536591485	18.318839772078185
29	16.54678945095362	22.68039398709929	38.79970464899205	21.973111912955044
30	18.61419078003316	26.68641246046894	35.12698700176932	19.572409757728586
31	24.953426489641817	25.113027487148052	30.079326822608284	19.854219200601847
32	25.625266442831467	25.292132796500372	31.06167542038758	18.020925340280584
33	24.189024645091184	26.01819473662213	30.26667966954123	19.526100948745455
34	19.608520597946473	25.681271681132365	32.25026818428788	22.45993953663328
35	20.28966689421697	25.7153206369551	32.32182114546037	21.673191323367558
36	25.292522882737288	25.61629445938227	30.022931498070466	19.068251159809975
37	20.313239248248095	30.286462614413683	30.439599325708077	18.96069881163014
38	19.92499198930049	30.492316694297777	28.35559146825673	21.227099848145002
39	21.03367280123713	29.554326474317	29.677872358210617	19.73412836623525
40	22.30769445102328	26.76526560693239	28.34422324078073	22.5828167012636
41	18.546260048203514	26.488248652112734	28.972652168461526	25.992839131222222
42	21.24092004625308	26.354003260006408	27.44930968667716	24.955767007063347
43	21.740509062539182	26.240711071483304	28.712464648434775	23.306315217542735
44	19.884534473871188	29.294807673553546	29.32958107524485	21.491076777330417
45	17.801919781551707	33.017512085707516	26.869920171637947	22.31064796110283
46	20.3213196060129	30.569720949024088	29.230109084829824	19.87885036013319
47	20.85724236893799	26.673651067861076	30.357012496691233	22.1120940665097
48	21.603421613563857	24.27768567408295	31.73100767633988	22.38788503601332
49	19.84937098594296	25.159280569525905	32.497582858496216	22.493765586034915
50	19.469092631549618	30.0250491090709	29.56597333481938	20.939884924560108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	600.0
1	1712.5
2	2825.0
3	18709.5
4	34594.0
5	25152.0
6	15710.0
7	15713.0
8	15716.0
9	15938.5
10	16161.0
11	15901.0
12	15641.0
13	15004.5
14	14368.0
15	13073.5
16	11779.0
17	10727.5
18	9676.0
19	9277.5
20	8879.0
21	8849.5
22	8820.0
23	10024.0
24	11228.0
25	11887.5
26	12547.0
27	16657.5
28	20768.0
29	25016.5
30	29265.0
31	33728.5
32	38192.0
33	42902.5
34	47613.0
35	54167.0
36	60721.0
37	61442.5
38	62164.0
39	75692.0
40	89220.0
41	110335.5
42	131451.0
43	146739.0
44	162027.0
45	170865.5
46	179704.0
47	162190.5
48	144677.0
49	136132.0
50	127587.0
51	122557.0
52	117527.0
53	106267.5
54	95008.0
55	87285.0
56	79562.0
57	69699.0
58	59836.0
59	56557.5
60	53279.0
61	48030.5
62	42782.0
63	36071.0
64	29360.0
65	23790.5
66	18221.0
67	14487.5
68	10754.0
69	9057.0
70	7360.0
71	5787.0
72	4214.0
73	3244.5
74	2275.0
75	1792.0
76	1309.0
77	869.5
78	430.0
79	353.0
80	276.0
81	219.0
82	162.0
83	148.0
84	134.0
85	81.5
86	29.0
87	22.0
88	15.0
89	10.5
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1794475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.746882879848698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16455218947152	15.830000232010125
2	7.439165483482587	2.938006590522874
3	2.4823575273742526	1.4705647007691052
4	1.3241783908910993	1.0459358238781218
5	0.7651009364094551	0.7554179291270038
6	0.5940273011898193	0.7038112526416781
7	0.447504119191362	0.6185768000945174
8	0.3452332040609555	0.5453823717461275
9	0.2839610370907318	0.5046610807683947
>10	3.669284924844533	17.108751431968724
>50	1.2663245973611856	18.04125085469938
>100	1.2145950566944337	38.660984534069414
>500	0.0028578707216046742	0.35779180361625096
>1k	5.715741443209348E-4	0.31315247922185757
>5k	0.0	0.0
>10k+	2.857870721604674E-4	1.1057121148664364
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19593	1.0918513771437328	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4270	0.237952604522214	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04519427687763831	0.0
2	0.0	0.0	0.0	0.14667242508254502	0.0
3	0.0	0.0	0.0	0.21348862480669833	0.0
4	0.0	0.0	0.0	0.3193691748282924	0.0
5	0.0	0.0	0.0	0.5681884673790384	0.0
6	0.0	0.0	0.0	0.7719806628679697	0.0
7	0.0	0.0	0.0	0.9068390476323158	0.0
8	0.0	0.0	0.0	1.275804901154934	0.0
9	0.0	0.0	0.0	1.4328982014238147	0.0
10	0.0	0.0	0.0	1.7254628791150615	0.0
11	0.0	0.0	0.0	1.9979102523022054	0.0
12	0.0	0.0	0.0	2.2471753576951476	0.0
13	5.572660527452319E-5	0.0	0.0	2.3376753646609734	0.0
14	5.572660527452319E-5	0.0	0.0	2.370108248930746	0.0
15	5.572660527452319E-5	0.0	0.0	2.4276181055740538	0.0
16	5.572660527452319E-5	0.0	0.0	2.549715097730534	0.0
17	5.572660527452319E-5	0.0	0.0	2.713829950264005	0.0
18	5.572660527452319E-5	0.0	0.0	2.9366249181515487	0.0
19	5.572660527452319E-5	0.0	0.0	3.054486688307165	0.0
20	5.572660527452319E-5	0.0	0.0	3.180484542832862	0.0
21	5.572660527452319E-5	0.0	0.0	3.3372992100753702	0.0
22	5.572660527452319E-5	0.0	0.0	3.509828780005294	0.0
23	5.572660527452319E-5	0.0	0.0	3.686314938909709	0.0
24	5.572660527452319E-5	0.0	0.0	3.8163808356204463	0.0
25	5.572660527452319E-5	0.0	0.0	3.9305646498279443	0.0
26	5.572660527452319E-5	0.0	0.0	4.04435837779852	0.0
27	5.572660527452319E-5	0.0	0.0	4.160102536953705	0.0
28	5.572660527452319E-5	0.0	0.0	4.286657657532147	0.0
29	5.572660527452319E-5	0.0	0.0	4.435224787194026	0.0
30	5.572660527452319E-5	0.0	0.0	4.611989579124813	0.0
31	5.572660527452319E-5	0.0	0.0	4.7763273380793825	0.0
32	5.572660527452319E-5	0.0	0.0	4.9256189136098305	0.0
33	5.572660527452319E-5	0.0	0.0	5.0681675699020605	0.0
34	5.572660527452319E-5	0.0	0.0	5.22944036556653	0.0
35	5.572660527452319E-5	0.0	0.0	5.425096476685382	0.0
36	5.572660527452319E-5	0.0	0.0	5.5938923640619125	0.0
37	5.572660527452319E-5	0.0	0.0	5.765530308307444	0.0
38	5.572660527452319E-5	0.0	0.0	5.944914250686134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.8576646E-4	44.0	1
GTCGATC	20	7.8576646E-4	44.0	40
CTCCGTA	30	2.5285153E-6	44.0	18
CTAACGT	20	7.8576646E-4	44.0	30
TACGCCC	25	4.4438424E-5	44.0	14
CGTACCC	20	7.8576646E-4	44.0	14
TTACGCG	30	2.5285153E-6	44.0	1
CGACTCA	30	2.5285153E-6	44.0	18
GCGCTAA	30	2.5285153E-6	44.0	22
TTCGATA	25	4.4438424E-5	44.0	15
CGTTTTT	14165	0.0	43.20791	1
CGACGGT	275	0.0	43.2	28
GCGATAT	155	0.0	41.16129	9
CACGACG	285	0.0	40.912277	26
GCCGACC	70	0.0	40.857143	14
TGTAGCG	205	0.0	40.780487	1
GGCGATA	725	0.0	40.66207	8
TACGTAG	65	0.0	40.615387	1
ACGAACG	65	0.0	40.615387	20
TCACGAC	305	0.0	40.393444	25
>>END_MODULE