FastQCFastQC Report
Thu 26 May 2016
SRR1545868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545868_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2089783
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196080.9382792376050528No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA43420.20777276875158807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT254.4440945E-544.037
CGCGATT207.857963E-444.028
TCTAGCG408.3182385E-944.01
AATGCGC302.5287154E-644.027
TACGATG454.802132E-1044.01
TTACGCC207.857963E-444.032
TACGTTG351.4468424E-744.01
CGTTTTT137100.043.085341
GTTAGCG1150.042.086961
CTAACGG2000.041.8000032
ACTACGG750.041.0666662
AATACGG2350.039.3191452
CGCACGG1350.039.111112
TTAGGGA35400.038.9039544
TAATGCG1700.038.823531
ATACGTC404.126705E-738.513
ACGGGAT9700.038.3298955
GCGATAA2300.038.260879
CGGGATA4900.038.1632656
CTACGGG3300.038.03
Produced by FastQC (version 0.11.5)