##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545868_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2089783
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.472188739213593	31.0	31.0	33.0	30.0	34.0
2	31.882077708546774	31.0	31.0	34.0	30.0	34.0
3	31.93990715782452	31.0	31.0	34.0	30.0	34.0
4	35.700810084109214	37.0	35.0	37.0	33.0	37.0
5	35.630044363457834	37.0	35.0	37.0	33.0	37.0
6	35.68176887265329	37.0	35.0	37.0	33.0	37.0
7	35.981851704220006	37.0	35.0	37.0	35.0	37.0
8	35.96610461468966	37.0	35.0	37.0	35.0	37.0
9	37.72554758077752	39.0	38.0	39.0	35.0	39.0
10	37.230751231108684	39.0	37.0	39.0	34.0	39.0
11	36.97947155278802	39.0	37.0	39.0	33.0	39.0
12	36.46528802272772	39.0	35.0	39.0	32.0	39.0
13	36.23279833360689	38.0	35.0	39.0	32.0	39.0
14	37.32255262867006	39.0	35.0	41.0	32.0	41.0
15	37.438105774618705	39.0	35.0	41.0	32.0	41.0
16	37.55765167962415	39.0	35.0	41.0	33.0	41.0
17	37.460918669546075	39.0	35.0	41.0	32.0	41.0
18	37.349799955306366	39.0	35.0	41.0	32.0	41.0
19	37.25202903842169	39.0	35.0	41.0	32.0	41.0
20	37.05516840743752	38.0	35.0	41.0	32.0	41.0
21	36.92576454110307	38.0	35.0	41.0	32.0	41.0
22	36.82763712787404	38.0	35.0	41.0	32.0	41.0
23	36.747280459262996	38.0	35.0	41.0	31.0	41.0
24	36.80336522978702	38.0	35.0	41.0	32.0	41.0
25	36.75751405767967	38.0	35.0	41.0	32.0	41.0
26	36.52641255096821	38.0	35.0	41.0	31.0	41.0
27	36.54460199934634	38.0	35.0	41.0	31.0	41.0
28	36.5184978536049	38.0	35.0	41.0	31.0	41.0
29	36.4890790096388	38.0	35.0	41.0	31.0	41.0
30	36.36895170455497	38.0	35.0	40.0	31.0	41.0
31	36.21596883504172	38.0	35.0	40.0	30.0	41.0
32	36.011847641597235	38.0	35.0	41.0	30.0	41.0
33	35.734087701928864	38.0	35.0	41.0	29.0	41.0
34	35.464514736697545	38.0	35.0	40.0	27.0	41.0
35	35.22569376820464	38.0	34.0	40.0	25.0	41.0
36	35.04993341413917	38.0	34.0	40.0	24.0	41.0
37	34.98466060830239	38.0	34.0	40.0	24.0	41.0
38	34.91137070212553	38.0	34.0	40.0	24.0	41.0
39	34.81075499226475	38.0	34.0	40.0	23.0	41.0
40	34.68277424019623	38.0	34.0	40.0	23.0	41.0
41	34.63821219715157	38.0	34.0	40.0	23.0	41.0
42	34.610015489646536	38.0	34.0	40.0	23.0	41.0
43	34.51507022499465	37.0	34.0	40.0	23.0	41.0
44	34.40746144456147	37.0	34.0	40.0	23.0	41.0
45	34.305974352360984	37.0	34.0	40.0	23.0	41.0
46	34.240976216190866	37.0	33.0	40.0	23.0	41.0
47	34.195636580448785	37.0	33.0	40.0	23.0	41.0
48	34.103746656949546	37.0	33.0	40.0	23.0	41.0
49	34.036464551582625	36.0	33.0	40.0	22.0	41.0
50	33.88975123254424	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	2.0
12	5.0
13	10.0
14	18.0
15	36.0
16	134.0
17	389.0
18	825.0
19	1644.0
20	2936.0
21	4717.0
22	7305.0
23	10823.0
24	16087.0
25	24766.0
26	36591.0
27	46075.0
28	48242.0
29	48760.0
30	52642.0
31	60439.0
32	73166.0
33	93299.0
34	157677.0
35	246439.0
36	139766.0
37	180984.0
38	291945.0
39	544059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.202617688056606	22.465825399096463	30.185717847259742	16.145839065587193
2	33.25594092783796	24.712183035272083	30.349036239647848	11.682839797242107
3	27.932708802780006	24.3589406172794	34.960711231740326	12.747639348200268
4	25.021928114067343	26.930403778765545	34.30102551317529	13.746642593991817
5	22.926782350129177	31.957050086061567	31.796459249596726	13.319708314212528
6	21.084820768472134	40.31542987956166	28.55846755380822	10.041281798157991
7	85.16563681492289	3.9620860156293745	9.468303646837974	1.4039735226097638
8	86.44648750611906	2.9572926949831633	9.192054868854804	1.4041649300429757
9	81.03736129540722	6.1303494190545145	10.506736823871186	2.3255524616670726
10	37.93475207712954	37.80995443067533	15.015721728045447	9.239571764149675
11	37.02470543592325	21.155593666902256	27.320827090659648	14.498873806514839
12	34.17531868141334	21.282209683971974	29.485023086129036	15.057448548485656
13	22.232978256594105	31.478292243740142	29.97923707868233	16.309492420983425
14	16.30834397638415	34.06018711033634	32.72253626333452	16.908932649944994
15	16.49850726128024	23.781703650570417	43.84134620675927	15.878442881390079
16	19.182183030486897	21.365089102552755	40.85902698988364	18.59370087707671
17	18.699884150651048	20.832641475215368	32.470165562644546	27.99730881148904
18	21.521612531061837	23.426068639662585	35.07211035786969	19.980208471405884
19	25.89479386137221	24.219117487318062	29.956842408996533	19.929246242313198
20	27.70684803158988	23.614461405801464	29.73433126788762	18.944359294721032
21	23.067897480264698	25.1523722798013	31.307796072606582	20.471934167327422
22	22.07468430932781	22.905966791767373	30.990298992766235	24.02904990613858
23	19.966140025064803	26.590081362514674	30.36631076049523	23.077467851925295
24	19.45833610475346	23.66202615295464	37.6509905573928	19.228647184899103
25	18.821427870740646	25.786983624615573	34.626418149635626	20.765170355008152
26	18.828174982761368	30.33822171967137	30.57269582535603	20.26090747221123
27	18.449475376151494	29.178101266973655	32.254975755855995	20.117447601018863
28	16.89318938856331	26.929446741599488	37.70893915779772	18.46842471203948
29	18.71653659734049	23.63503770487175	35.949761291004855	21.698664406782907
30	19.65381094592118	27.093004393279113	33.138464615704116	20.11472004509559
31	25.488244473229997	26.53868846669726	29.051963768486967	18.921103291585776
32	25.894937416947116	25.37521838391833	30.08350627792455	18.64633792121
33	24.809418011343762	26.67913367081654	29.317398026493656	19.19405029134604
34	20.795939099896977	26.928298297000214	30.761902073086056	21.513860530016753
35	21.745798487211353	25.83689311282559	31.351819782245332	21.065488617717726
36	25.767651473861164	26.647025073895232	28.50090176826972	19.084421683973886
37	21.61765121067594	30.475652256717563	28.81112536564801	19.095571166958482
38	20.40886541808408	29.90851203211051	27.885144055626824	21.79747849417858
39	21.95036518145664	29.269928983057092	28.200679209276753	20.579026626209515
40	23.470188053017946	26.851687471857126	28.36639019458001	21.31173428054492
41	19.096719611557756	26.2450216122918	29.403244260289224	25.25501451586122
42	22.02606682129197	26.240427833894714	27.199139814995142	24.534365529818167
43	22.46448554706398	26.47126519834834	28.185701577627913	22.87854767695976
44	20.83728310547076	28.651252307057717	29.40061240808256	21.11085217938896
45	19.333107791574534	31.940062676364008	26.172191083954647	22.55463844810681
46	20.905089188686098	30.737832588359655	28.19072602274973	20.166352200204518
47	21.718522927978647	27.152627808724638	29.47291656597838	21.655932697318335
48	21.8585374653732	25.384740903720626	31.15763694125179	21.59908468965438
49	20.96370771510726	25.78057147560297	31.69410412468663	21.561616684603138
50	19.89426653389371	29.610538510457786	29.305817876784335	21.18937707886417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	518.0
1	1676.0
2	2834.0
3	20018.0
4	37202.0
5	26968.5
6	16735.0
7	16466.0
8	16197.0
9	16151.5
10	16106.0
11	15629.5
12	15153.0
13	14203.5
14	13254.0
15	12073.5
16	10893.0
17	10114.0
18	9335.0
19	9190.5
20	9046.0
21	9045.0
22	9044.0
23	11087.5
24	13131.0
25	14183.0
26	15235.0
27	19553.5
28	23872.0
29	29713.0
30	35554.0
31	41679.5
32	47805.0
33	53045.0
34	58285.0
35	66078.5
36	73872.0
37	76654.0
38	79436.0
39	92233.0
40	105030.0
41	124862.0
42	144694.0
43	160912.5
44	177131.0
45	176540.0
46	175949.0
47	169420.0
48	162891.0
49	154189.5
50	145488.0
51	142252.5
52	139017.0
53	128208.5
54	117400.0
55	107881.5
56	98363.0
57	93174.0
58	87985.0
59	81912.5
60	75840.0
61	66126.0
62	56412.0
63	47729.5
64	39047.0
65	30980.5
66	22914.0
67	18915.5
68	14917.0
69	12178.5
70	9440.0
71	7811.5
72	6183.0
73	4522.0
74	2861.0
75	2555.5
76	2250.0
77	1803.0
78	1356.0
79	943.0
80	530.0
81	400.0
82	270.0
83	198.5
84	127.0
85	100.0
86	73.0
87	60.5
88	48.0
89	37.5
90	27.0
91	20.0
92	13.0
93	11.0
94	9.0
95	10.0
96	11.0
97	5.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2089783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.142707084212343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29824506128092	14.568275395231204
2	7.213456914459979	2.617432717272671
3	2.3314373228219916	1.2689575329947882
4	1.1634879973037981	0.8443528772431861
5	0.7242994513228742	0.6570376393303312
6	0.5327802600535481	0.57996457190412
7	0.38219770459450236	0.4853870701901462
8	0.33075248670290286	0.48005963869004836
9	0.28916964622153307	0.4721688170138326
>10	3.836629762211144	17.045859079632304
>50	1.5079451689652241	19.69414886555148
>100	1.3855913087679554	39.684856515550635
>500	0.0029384045487390463	0.31807050297082934
>1k	8.013830587470126E-4	0.33314286719815345
>5k	0.0	0.0
>10k+	2.6712768624900416E-4	0.9502859092262719
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19608	0.9382792376050528	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4342	0.20777276875158807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.035218967711001574	0.0
2	0.0	0.0	0.0	0.13159261033322597	0.0
3	0.0	0.0	0.0	0.18308120986724458	0.0
4	0.0	0.0	0.0	0.25825647926124384	0.0
5	0.0	0.0	0.0	0.44085917054545853	0.0
6	0.0	0.0	0.0	0.6016892663018122	0.0
7	0.0	0.0	0.0	0.7159595039293554	0.0
8	0.0	0.0	0.0	1.0100570250595398	0.0
9	0.0	0.0	0.0	1.1435158578665823	0.0
10	0.0	0.0	0.0	1.3945467065240744	0.0
11	0.0	0.0	0.0	1.605334142348751	0.0
12	0.0	0.0	0.0	1.8145424668494288	0.0
13	0.0	0.0	0.0	1.89134469942573	0.0
14	0.0	0.0	0.0	1.918333147508617	0.0
15	4.785185830299127E-5	0.0	0.0	1.9691039691680907	0.0
16	4.785185830299127E-5	0.0	0.0	2.076244279908488	0.0
17	4.785185830299127E-5	0.0	0.0	2.2174072619023124	0.0
18	4.785185830299127E-5	0.0	0.0	2.4139348439526973	0.0
19	4.785185830299127E-5	0.0	0.0	2.503274263404382	0.0
20	4.785185830299127E-5	0.0	0.0	2.6116108706023544	0.0
21	4.785185830299127E-5	0.0	0.0	2.734255183432921	0.0
22	4.785185830299127E-5	0.0	0.0	2.8833137220467386	0.0
23	4.785185830299127E-5	0.0	0.0	3.036152557466493	0.0
24	4.785185830299127E-5	0.0	0.0	3.149513609786279	0.0
25	4.785185830299127E-5	0.0	0.0	3.2461265117000186	0.0
26	4.785185830299127E-5	0.0	0.0	3.343265784055091	0.0
27	4.785185830299127E-5	0.0	0.0	3.4394480192441033	0.0
28	4.785185830299127E-5	0.0	0.0	3.5431429961866856	0.0
29	9.570371660598254E-5	0.0	0.0	3.656456196648169	0.0
30	9.570371660598254E-5	0.0	0.0	3.803696364646473	0.0
31	9.570371660598254E-5	0.0	0.0	3.934858308254972	0.0
32	9.570371660598254E-5	0.0	0.0	4.056019213478145	0.0
33	9.570371660598254E-5	0.0	0.0	4.178807081883622	0.0
34	1.435555749089738E-4	0.0	0.0	4.313940729731269	0.0
35	1.435555749089738E-4	0.0	0.0	4.466205342851387	0.0
36	1.435555749089738E-4	0.0	0.0	4.606363435820849	0.0
37	1.435555749089738E-4	0.0	0.0	4.743267602425706	0.0
38	1.435555749089738E-4	0.0	0.0	4.887971621933952	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	25	4.4440945E-5	44.0	37
CGCGATT	20	7.857963E-4	44.0	28
TCTAGCG	40	8.3182385E-9	44.0	1
AATGCGC	30	2.5287154E-6	44.0	27
TACGATG	45	4.802132E-10	44.0	1
TTACGCC	20	7.857963E-4	44.0	32
TACGTTG	35	1.4468424E-7	44.0	1
CGTTTTT	13710	0.0	43.08534	1
GTTAGCG	115	0.0	42.08696	1
CTAACGG	200	0.0	41.800003	2
ACTACGG	75	0.0	41.066666	2
AATACGG	235	0.0	39.319145	2
CGCACGG	135	0.0	39.11111	2
TTAGGGA	3540	0.0	38.903954	4
TAATGCG	170	0.0	38.82353	1
ATACGTC	40	4.126705E-7	38.5	13
ACGGGAT	970	0.0	38.329895	5
GCGATAA	230	0.0	38.26087	9
CGGGATA	490	0.0	38.163265	6
CTACGGG	330	0.0	38.0	3
>>END_MODULE