FastQCFastQC Report
Thu 26 May 2016
SRR1545867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545867_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2015383
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225191.1173558574226339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA207.857897E-444.012
CGTTTTT132000.042.5500031
ACGGGTA1250.042.245
CGACGGT2750.038.428
AGGGATC25400.038.1968546
TATTACG750.038.1333351
TACGGGA10800.038.092594
ATAGGGA34650.037.0158734
CGAGGGA16000.036.7124984
TTTAGCG1200.036.6666641
ATTACGC301.3014434E-436.66666412
CACGTAA301.3014434E-436.66666440
GTTTTTT161600.036.5668332
ACGGGAT10500.036.457145
CACGACG2850.036.28070426
CGGGTAT1400.036.1428576
GCGTTAG1400.036.1428571
TAGGGAT37550.036.0905465
AAGGGAT32850.036.030445
AAGGGCG6900.036.0289845
Produced by FastQC (version 0.11.5)