##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545867_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2015383
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51154991383772	31.0	31.0	33.0	30.0	34.0
2	31.935434108554055	31.0	31.0	34.0	30.0	34.0
3	32.0119902767861	33.0	31.0	34.0	30.0	34.0
4	35.768877677344705	37.0	35.0	37.0	35.0	37.0
5	35.69984464491365	37.0	35.0	37.0	33.0	37.0
6	35.74013276880871	37.0	35.0	37.0	33.0	37.0
7	35.99324942207015	37.0	35.0	37.0	35.0	37.0
8	35.9699392125467	37.0	35.0	37.0	35.0	37.0
9	37.74010696726131	39.0	38.0	39.0	35.0	39.0
10	37.27650129032546	39.0	37.0	39.0	34.0	39.0
11	37.06032600255138	39.0	37.0	39.0	33.0	39.0
12	36.688069215628	39.0	35.0	39.0	33.0	39.0
13	36.551146357789065	39.0	35.0	39.0	32.0	39.0
14	37.63534424970341	40.0	36.0	41.0	33.0	41.0
15	37.71809427786182	40.0	36.0	41.0	33.0	41.0
16	37.79219731435663	40.0	36.0	41.0	33.0	41.0
17	37.68814265080136	40.0	36.0	41.0	33.0	41.0
18	37.57031690750592	39.0	36.0	41.0	33.0	41.0
19	37.46209628641305	39.0	36.0	41.0	32.0	41.0
20	37.29353328870989	39.0	35.0	41.0	32.0	41.0
21	37.19027847312397	39.0	35.0	41.0	32.0	41.0
22	37.116586772836726	39.0	35.0	41.0	32.0	41.0
23	36.997873853257666	39.0	35.0	41.0	32.0	41.0
24	37.05257412610903	39.0	35.0	41.0	32.0	41.0
25	37.021646505899874	39.0	35.0	41.0	32.0	41.0
26	36.79753029573039	39.0	35.0	41.0	31.0	41.0
27	36.795891401287	39.0	35.0	41.0	31.0	41.0
28	36.75133510603195	39.0	35.0	41.0	31.0	41.0
29	36.68389234205112	38.0	35.0	41.0	31.0	41.0
30	36.55696907237979	38.0	35.0	41.0	31.0	41.0
31	36.407393036460064	38.0	35.0	41.0	30.0	41.0
32	36.23243274355296	38.0	35.0	41.0	30.0	41.0
33	35.97634395050469	38.0	35.0	41.0	29.0	41.0
34	35.68420841100674	38.0	35.0	41.0	27.0	41.0
35	35.44400344748368	38.0	35.0	41.0	26.0	41.0
36	35.309416125867884	38.0	34.0	41.0	25.0	41.0
37	35.240143932939795	38.0	34.0	40.0	25.0	41.0
38	35.12866636267151	38.0	34.0	40.0	24.0	41.0
39	34.99188789426129	38.0	34.0	40.0	24.0	41.0
40	34.88706315375291	38.0	34.0	40.0	23.0	41.0
41	34.816678021001465	38.0	34.0	40.0	23.0	41.0
42	34.7950712097899	38.0	34.0	40.0	23.0	41.0
43	34.703483159280395	38.0	34.0	40.0	23.0	41.0
44	34.57907157101157	38.0	34.0	40.0	23.0	41.0
45	34.49424104500236	37.0	34.0	40.0	23.0	41.0
46	34.43771928214141	37.0	34.0	40.0	23.0	41.0
47	34.367335141757174	37.0	33.0	40.0	23.0	41.0
48	34.228106022527726	37.0	33.0	40.0	23.0	41.0
49	34.15930619639046	37.0	33.0	40.0	23.0	41.0
50	34.03462220332314	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	4.0
13	6.0
14	22.0
15	60.0
16	149.0
17	386.0
18	797.0
19	1604.0
20	2812.0
21	4562.0
22	6908.0
23	10097.0
24	14757.0
25	22200.0
26	33207.0
27	42573.0
28	44971.0
29	45747.0
30	49014.0
31	55757.0
32	67184.0
33	86137.0
34	138964.0
35	191780.0
36	145972.0
37	191116.0
38	305987.0
39	552609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.86663924425283	23.073778036234305	29.350847952969733	16.708734766543134
2	33.7302636769289	24.4559967013714	28.709034461439835	13.104705160259861
3	28.13495003183018	24.21733238793817	34.60503537044819	13.042682209783452
4	25.50453189294541	26.89037269839033	33.69716823055469	13.907927178109569
5	22.87714047404389	31.03385311873723	31.85240720994471	14.236599197274167
6	21.07902071219217	40.113268793078035	28.742427617976336	10.06528287675345
7	84.96459481895005	3.5812051605079533	9.782011657337588	1.6721883632044132
8	85.74831682116996	2.858067176313386	9.789404793034375	1.6042112094822671
9	80.19498030895367	6.167959142257328	10.996817974548758	2.640242574240231
10	35.37124209145359	37.780411961398904	16.02439834016661	10.823947606980907
11	33.5357100858745	22.137380339121645	28.30251123483725	16.02439834016661
12	30.621077978726625	21.43106297909628	30.809826221616436	17.13803282056066
13	23.944282550760825	25.623814431301646	32.023937881782274	18.407965136155262
14	18.951087708887098	27.99279342933825	34.31749697203956	18.738621889735104
15	19.061835889257775	23.734248031267505	39.1660542934023	18.037861786072423
16	23.210228527282407	21.926403070781085	36.23107865849816	18.632289743438342
17	22.24554836475251	22.271399530511076	32.154334932863875	23.328717171872544
18	23.351640854368625	23.266843076477276	33.46232453087081	19.919191538283293
19	25.548096813360043	24.38156916079971	30.510280179995565	19.560053845844685
20	26.69373513619992	24.12082467699688	30.309425057172756	18.876015129630446
21	24.689302231883467	24.37898900605989	31.908724048977295	19.02298471307935
22	23.717625880539828	22.4001591757001	31.84843774111422	22.033777202645847
23	22.21850635834479	24.885741320632356	31.937552316358726	20.958200004664125
24	21.513429457328954	23.68433196072409	34.664676639626315	20.13756194232064
25	21.48594088567781	25.275394304705358	32.023292843097316	21.215371966519513
26	21.122982579489854	27.04423923393221	30.60331460571018	21.229463580867755
27	20.308001010229816	26.021505589756387	32.378659540146955	21.291833859866834
28	19.447817114662573	25.842631400582423	34.528771950542406	20.180779534212604
29	20.992039726444055	23.94333980191358	33.82290115576047	21.241719315881895
30	22.06250623330652	25.490787607119834	32.619953626680385	19.826752532893252
31	25.187420951749615	24.857210763413207	30.102466876023072	19.852901408814105
32	25.6649480520576	24.90077568382784	30.254249440428943	19.180026823685623
33	24.921317685025627	25.593646468189917	29.26495857115	20.220077275634456
34	21.697166245820274	26.280662286027024	31.393139666256985	20.62903180189572
35	22.60151048212672	26.20886451855553	30.489291613554347	20.7003333857634
36	24.540893715983515	27.65186567515951	28.16263707692285	19.64460353193413
37	22.51576995538813	28.932366701515296	28.5695076320481	19.98235571104847
38	22.40849506024413	29.062168332272325	27.37742652389149	21.15191008359205
39	22.740243417752357	27.761621488322568	28.022961392449968	21.475173701475104
40	23.391137069232002	26.574254124402163	28.17990426633548	21.854704540030355
41	20.6501196050577	26.077872047149352	29.05001183397895	24.221996513814002
42	21.516704269114108	26.614444996310876	28.324541786846467	23.544308947728545
43	21.710860913285465	26.566215950020418	28.765351300472414	22.957571836221703
44	21.80518541636999	27.505888458918232	29.230920375928545	21.458005748783236
45	21.068005436187565	28.594763377482096	27.83282383546949	22.504407350860852
46	21.56413942163847	28.47746557354111	28.670381758702934	21.288013246117487
47	22.014525278817974	26.694429793245256	29.941901861829734	21.349143066107036
48	21.67468912856762	25.850719193324544	30.996540111730624	21.47805156637721
49	21.8311854372097	26.119005667905306	30.433768668287865	21.61604022659713
50	20.600798954838854	27.58631982109604	30.112638639901203	21.700242584163902
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	851.0
1	2269.0
2	3687.0
3	23069.0
4	42451.0
5	29579.5
6	16708.0
7	15831.5
8	14955.0
9	14608.0
10	14261.0
11	13759.0
12	13257.0
13	12440.0
14	11623.0
15	10723.0
16	9823.0
17	9216.5
18	8610.0
19	9130.5
20	9651.0
21	10044.0
22	10437.0
23	11651.5
24	12866.0
25	14528.0
26	16190.0
27	19879.5
28	23569.0
29	29052.0
30	34535.0
31	39295.5
32	44056.0
33	50206.0
34	56356.0
35	60642.0
36	64928.0
37	71037.5
38	77147.0
39	84806.5
40	92466.0
41	106901.5
42	121337.0
43	129223.0
44	137109.0
45	138286.0
46	139463.0
47	138590.0
48	137717.0
49	137264.5
50	136812.0
51	134110.0
52	131408.0
53	122866.0
54	114324.0
55	109173.5
56	104023.0
57	100736.5
58	97450.0
59	94558.0
60	91666.0
61	83880.0
62	76094.0
63	65251.0
64	54408.0
65	45473.5
66	36539.0
67	29439.5
68	22340.0
69	18963.5
70	15587.0
71	12548.5
72	9510.0
73	7434.5
74	5359.0
75	4045.5
76	2732.0
77	2194.5
78	1657.0
79	1176.0
80	695.0
81	534.5
82	374.0
83	284.0
84	194.0
85	136.0
86	78.0
87	53.5
88	29.0
89	28.0
90	27.0
91	18.5
92	10.0
93	10.0
94	10.0
95	6.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2015383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.830125367770876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33880722037352	15.138081176060997
2	6.38406244551392	2.5319351730043795
3	2.4649266290015825	1.4663941222637469
4	1.4818874397581316	1.1754405484531505
5	0.9982361982535637	0.9897574479007574
6	0.8334861603681051	0.9916881031440903
7	0.6741128042956629	0.9357418994841813
8	0.6115756928309006	0.9702098128574473
9	0.49632295229937834	0.8857931730298952
>10	7.3346372239388895	34.68534438793519
>50	1.8651439721468686	25.80563991936983
>100	0.5142666793929207	12.93333126303271
>500	0.0017742072785625783	0.22185238670403623
>1k	5.069163653035937E-4	0.13696143039612566
>5k	0.0	0.0
>10k+	2.5345818265179687E-4	1.1318291563634326
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22519	1.1173558574226339	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013496194023666966	0.0
2	0.0	0.0	0.0	0.0590954672139241	0.0
3	0.0	0.0	0.0	0.09343137259766505	0.0
4	0.0	0.0	0.0	0.1449352306732765	0.0
5	0.0	0.0	0.0	0.2761261755209804	0.0
6	0.0	0.0	0.0	0.42408812617750574	0.0
7	0.0	0.0	0.0	0.5253591997153891	0.0
8	0.0	0.0	0.0	0.7550922082800142	0.0
9	0.0	0.0	0.0	0.8752678771231076	0.0
10	0.0	0.0	0.0	1.0679359704830298	0.0
11	0.0	0.0	0.0	1.2293941151632222	0.0
12	0.0	0.0	0.0	1.3767110271347927	0.0
13	0.0	0.0	0.0	1.434268325176902	0.0
14	0.0	0.0	0.0	1.458233993240987	0.0
15	0.0	0.0	0.0	1.4914782946963432	0.0
16	0.0	0.0	0.0	1.5572722405617196	0.0
17	0.0	0.0	0.0	1.6398868105962985	0.0
18	0.0	0.0	0.0	1.746566285415725	0.0
19	0.0	0.0	0.0	1.804917477223932	0.0
20	0.0	0.0	0.0	1.8744824184782745	0.0
21	0.0	0.0	0.0	1.9599252350545777	0.0
22	0.0	0.0	0.0	2.0554901971486315	0.0
23	0.0	0.0	0.0	2.1516009612068774	0.0
24	4.961836038112855E-5	0.0	0.0	2.226326211940857	0.0
25	4.961836038112855E-5	0.0	0.0	2.2914751191212788	0.0
26	4.961836038112855E-5	0.0	0.0	2.355582040733697	0.0
27	4.961836038112855E-5	0.0	0.0	2.4308034750714875	0.0
28	4.961836038112855E-5	0.0	0.0	2.5086050641490973	0.0
29	4.961836038112855E-5	0.0	0.0	2.598066967916272	0.0
30	4.961836038112855E-5	0.0	0.0	2.7040517856903628	0.0
31	4.961836038112855E-5	0.0	0.0	2.7929182691329637	0.0
32	4.961836038112855E-5	0.0	0.0	2.8835213951889047	0.0
33	4.961836038112855E-5	0.0	0.0	2.9731321540372226	0.0
34	4.961836038112855E-5	0.0	0.0	3.0728650584032913	0.0
35	4.961836038112855E-5	0.0	0.0	3.1888231666139886	0.0
36	4.961836038112855E-5	0.0	0.0	3.2905904237556833	0.0
37	4.961836038112855E-5	0.0	0.0	3.39201035237471	0.0
38	4.961836038112855E-5	0.0	0.0	3.517544804138965	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGA	20	7.857897E-4	44.0	12
CGTTTTT	13200	0.0	42.550003	1
ACGGGTA	125	0.0	42.24	5
CGACGGT	275	0.0	38.4	28
AGGGATC	2540	0.0	38.196854	6
TATTACG	75	0.0	38.133335	1
TACGGGA	1080	0.0	38.09259	4
ATAGGGA	3465	0.0	37.015873	4
CGAGGGA	1600	0.0	36.712498	4
TTTAGCG	120	0.0	36.666664	1
ATTACGC	30	1.3014434E-4	36.666664	12
CACGTAA	30	1.3014434E-4	36.666664	40
GTTTTTT	16160	0.0	36.566833	2
ACGGGAT	1050	0.0	36.45714	5
CACGACG	285	0.0	36.280704	26
CGGGTAT	140	0.0	36.142857	6
GCGTTAG	140	0.0	36.142857	1
TAGGGAT	3755	0.0	36.090546	5
AAGGGAT	3285	0.0	36.03044	5
AAGGGCG	690	0.0	36.028984	5
>>END_MODULE