FastQCFastQC Report
Thu 26 May 2016
SRR1545866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545866_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1813341
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254811.405196264795204No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA53820.29680021573438203No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA21930.12093698868552577No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCGA302.528528E-644.00000412
TAGCGAT302.528528E-644.00000414
CGTACCC302.528528E-644.00000437
TAGTGCG302.528528E-644.0000041
TCGACGA302.528528E-644.0000041
ACGCGAT207.857688E-444.027
CGAACTT207.857688E-444.025
AAATCCG254.443861E-544.01
GTCGATC254.443861E-544.038
TCCGCAT254.443861E-544.019
GCACGAC351.4466968E-744.044
TAAGTCG207.857688E-444.01
CTAAGCG454.802132E-1044.01
ACCTCGC254.443861E-544.026
CCCGTTA351.4466968E-744.028
AAACGCG207.857688E-444.01
CCGTATC351.4466968E-744.022
TACGCTA254.443861E-544.01
GCCGAAC254.443861E-544.035
CTCGGTT207.857688E-444.024
Produced by FastQC (version 0.11.5)