##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545866_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1813341
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53006908242851	31.0	31.0	33.0	30.0	34.0
2	31.92778854059992	31.0	31.0	34.0	30.0	34.0
3	31.917345937691806	31.0	31.0	34.0	30.0	34.0
4	35.70736502400817	37.0	35.0	37.0	33.0	37.0
5	35.65141140028268	37.0	35.0	37.0	33.0	37.0
6	35.69945586627115	37.0	35.0	37.0	33.0	37.0
7	35.994769875053834	37.0	35.0	37.0	35.0	37.0
8	35.993149109847515	37.0	35.0	37.0	35.0	37.0
9	37.77964321106731	39.0	38.0	39.0	35.0	39.0
10	37.329137762836666	39.0	37.0	39.0	34.0	39.0
11	37.04202463849877	39.0	37.0	39.0	33.0	39.0
12	36.22733617118898	38.0	35.0	39.0	32.0	39.0
13	35.85350025174526	38.0	35.0	39.0	31.0	39.0
14	36.89658701810636	39.0	35.0	41.0	32.0	41.0
15	37.12571215231994	39.0	35.0	41.0	32.0	41.0
16	37.2988423026888	39.0	35.0	41.0	33.0	41.0
17	37.22816833678828	39.0	35.0	41.0	32.0	41.0
18	37.06358098118335	38.0	35.0	40.0	32.0	41.0
19	36.87248344354426	37.0	35.0	40.0	32.0	41.0
20	36.596999681802814	37.0	35.0	40.0	32.0	41.0
21	36.43424430374651	37.0	35.0	40.0	32.0	41.0
22	36.32208724117527	37.0	35.0	40.0	31.0	41.0
23	36.28883866851298	36.0	35.0	40.0	31.0	41.0
24	36.29716694212506	37.0	35.0	40.0	32.0	41.0
25	36.25917243364596	36.0	35.0	40.0	31.0	41.0
26	36.028712194783	36.0	35.0	40.0	31.0	41.0
27	36.01048506596388	36.0	35.0	40.0	31.0	41.0
28	36.01782014524571	36.0	35.0	40.0	31.0	41.0
29	35.98658222584721	36.0	35.0	40.0	31.0	41.0
30	35.861705547936104	36.0	35.0	40.0	31.0	41.0
31	35.58931331724149	36.0	35.0	40.0	30.0	41.0
32	35.22936281703221	36.0	34.0	40.0	29.0	41.0
33	34.84384900578545	36.0	34.0	40.0	25.0	41.0
34	34.48235549739404	36.0	34.0	40.0	23.0	41.0
35	34.13270035806834	36.0	34.0	40.0	21.0	41.0
36	33.90967942598772	36.0	33.0	40.0	20.0	41.0
37	33.78319852691799	36.0	33.0	40.0	19.0	41.0
38	33.727317697002384	35.0	33.0	40.0	19.0	41.0
39	33.64419984989034	36.0	33.0	40.0	18.0	41.0
40	33.48277240739607	36.0	33.0	40.0	18.0	41.0
41	33.47453733191937	36.0	33.0	40.0	18.0	41.0
42	33.42617742608809	36.0	33.0	40.0	18.0	41.0
43	33.31839626413344	35.0	33.0	40.0	18.0	41.0
44	33.17169522996502	35.0	33.0	40.0	17.0	41.0
45	33.09127957731061	35.0	33.0	40.0	18.0	41.0
46	33.07648919866699	35.0	33.0	40.0	18.0	41.0
47	33.02070156688676	35.0	33.0	40.0	17.0	41.0
48	32.928243501911666	35.0	32.0	40.0	17.0	41.0
49	32.89787469648566	35.0	32.0	40.0	17.0	41.0
50	32.71519146150669	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	6.0
13	6.0
14	20.0
15	44.0
16	142.0
17	361.0
18	874.0
19	1770.0
20	3125.0
21	4989.0
22	7736.0
23	11818.0
24	17783.0
25	27839.0
26	42802.0
27	52754.0
28	52740.0
29	49478.0
30	50010.0
31	55469.0
32	66693.0
33	85693.0
34	154730.0
35	277338.0
36	108063.0
37	134508.0
38	210843.0
39	395703.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.431001118929096	22.181652540807274	29.26156746028463	20.125778879979002
2	35.98391036214369	24.194015356185076	27.641022841263723	12.181051440407513
3	25.991140110988503	23.552712920515226	38.210022273802885	12.246124694693385
4	24.042306438777924	26.04965089302012	36.47184947563641	13.436193192565545
5	21.261196873616157	30.155111476550744	35.40635765694373	13.177333992889368
6	19.553244535914647	38.10425066217551	32.37460576913002	9.967899032779824
7	81.10559459031698	4.046563773719339	13.454722525989322	1.3931191099743512
8	82.74874940786096	2.876127545784273	12.936232071077642	1.4388909752771266
9	78.11150798443316	5.505693634015885	14.390453863889913	1.9923445176610468
10	44.722200623048835	29.412835203086455	17.663969435423343	8.200994738441363
11	42.54257748542607	17.424521918381593	27.96622367221609	12.06667692397624
12	38.47070131872604	19.50091019835762	29.398662468890297	12.629726014026044
13	19.99177209361063	37.708903068976	29.398166147459303	12.901158689954068
14	13.729022836851975	39.2073526159724	32.648134024433354	14.415490522742274
15	13.100183583782643	21.403089656054764	51.81695003863035	13.679776721532244
16	15.176792451061328	17.79725931305805	49.42798955077947	17.597958685101148
17	15.423023027659994	18.345970228434698	33.059639637553	33.17136710635231
18	19.22854002639327	22.381504637020836	39.449392033820445	18.940563302765447
19	25.93395285277286	22.232828795025313	32.24054383593599	19.59267451626583
20	28.163373573972024	21.410755064822336	30.781744856593434	19.644126504612206
21	20.054804915346867	25.571803648624282	34.09965362278799	20.273737813240864
22	20.58399385443775	21.782334376159806	31.98499344579977	25.648678323602674
23	17.214247072117157	27.26370826005699	31.975287604482556	23.5467570633433
24	17.967552710714642	21.739540439442994	43.42988991039192	16.86301693945044
25	15.335725602630724	23.536775487897753	40.526078658123325	20.601420251348202
26	15.40267384898924	32.53618596833138	33.486972389638794	18.57416779304058
27	15.873021125094509	30.54384145067034	35.35689095432134	18.226246469913825
28	13.819573924595542	26.324998993570432	43.229982667352694	16.625444414481336
29	14.51111511844711	22.453085216735296	42.388497254515286	20.64730241030231
30	16.399397576076424	27.181208608860665	37.01476997431812	19.40462384074479
31	26.34060554523391	24.19782048715603	30.872626825290993	18.588947142319068
32	26.267260267098138	25.879412642189198	32.46339215845227	15.389934932260395
33	25.47358715211314	26.461652827570763	29.352339135330862	18.712420884985228
34	18.503193828408445	27.14966462457971	31.944405382109597	22.402736164902244
35	18.773633861474483	25.687115661091873	34.17426727791408	21.36498319951956
36	27.725232044055698	23.73221583805804	30.38794137451257	18.154610743373695
37	18.901243616065592	31.001504956872427	31.885177691344317	18.212073735717663
38	20.076201883705274	30.24753755636695	27.90059894967356	21.77566161025422
39	19.917103291658876	30.45251830736745	29.965737277213716	19.664641123759953
40	21.544761851190703	27.47332134441343	28.63879435803856	22.343122446357306
41	16.897924880097015	24.89895722867348	30.04884354349237	28.154274347737136
42	21.092282146601217	25.52459796585419	27.54468133682523	25.83843855071936
43	21.84034883676043	25.874008253273928	28.279292201521944	24.006350708443698
44	18.83435051653274	31.023177659359163	29.688238450462435	20.454233373645664
45	16.36233890922888	35.820786051823674	26.012757666649573	21.804117372297874
46	20.826088419111464	31.2362098469069	29.22175145215379	18.715950281827855
47	21.08318292036633	27.110510378356857	29.570113949885872	22.23619275139094
48	22.364795148844042	23.795193512968606	32.25377907409583	21.58623226409153
49	19.662214663430653	23.758024552469724	33.88970965747755	22.690051126622073
50	18.31067626000846	31.10683539389447	30.02375173781434	20.55873660828272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	610.0
1	1672.5
2	2735.0
3	23531.5
4	44328.0
5	32530.5
6	20733.0
7	20886.5
8	21040.0
9	21558.5
10	22077.0
11	21890.0
12	21703.0
13	20088.5
14	18474.0
15	17017.0
16	15560.0
17	14150.5
18	12741.0
19	11345.5
20	9950.0
21	9389.5
22	8829.0
23	9877.5
24	10926.0
25	13789.5
26	16653.0
27	18683.0
28	20713.0
29	25057.5
30	29402.0
31	30863.0
32	32324.0
33	34956.0
34	37588.0
35	42677.0
36	47766.0
37	52600.0
38	57434.0
39	70682.0
40	83930.0
41	107539.0
42	131148.0
43	158295.5
44	185443.0
45	186729.5
46	188016.0
47	175598.5
48	163181.0
49	146172.5
50	129164.0
51	117666.5
52	106169.0
53	97938.5
54	89708.0
55	79737.5
56	69767.0
57	62502.0
58	55237.0
59	51532.5
60	47828.0
61	42177.5
62	36527.0
63	32954.0
64	29381.0
65	23563.0
66	17745.0
67	14570.5
68	11396.0
69	9068.5
70	6741.0
71	5927.0
72	5113.0
73	3668.0
74	2223.0
75	1843.5
76	1464.0
77	1066.0
78	668.0
79	575.0
80	482.0
81	340.5
82	199.0
83	153.0
84	107.0
85	77.0
86	47.0
87	35.0
88	23.0
89	21.5
90	20.0
91	16.0
92	12.0
93	13.0
94	14.0
95	7.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1813341.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.84163511037751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.26030820678724	15.310770761891002
2	7.9060915001431535	2.979273823899089
3	2.5933795947576535	1.465905360813672
4	1.2926281672287927	0.9742091304128382
5	0.7352958902199096	0.6927088430841868
6	0.5100372054745753	0.5765960950961152
7	0.37420295200368875	0.49354168352157296
8	0.2675905679291953	0.4033475071920467
9	0.23205608249805865	0.3935084428414874
>10	2.4864308657116796	10.921663314777451
>50	0.9042700809076863	12.315914185803194
>100	1.426157455236782	50.306733357197096
>500	0.009478097314134104	1.0482813361540202
>1k	0.0014809527053334538	0.3951703652739894
>5k	2.9619054106669074E-4	0.30035403275026284
>10k+	2.9619054106669074E-4	1.422021759291982
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25481	1.405196264795204	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5382	0.29680021573438203	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2193	0.12093698868552577	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04036747638750792	0.0
2	0.0	0.0	0.0	0.14680084992287717	0.0
3	0.0	0.0	0.0	0.20911676292545087	0.0
4	0.0	0.0	0.0	0.32481480317270717	0.0
5	0.0	0.0	0.0	0.5714314075510343	0.0
6	0.0	0.0	0.0	0.7886547538493863	0.0
7	0.0	0.0	0.0	0.9351247228182675	0.0
8	0.0	0.0	0.0	1.3240201374148601	0.0
9	0.0	0.0	0.0	1.492990011255467	0.0
10	5.514682566599443E-5	0.0	0.0	1.8136136556775586	0.0
11	5.514682566599443E-5	0.0	0.0	2.085322065733913	0.0
12	5.514682566599443E-5	0.0	0.0	2.353942253552972	0.0
13	5.514682566599443E-5	0.0	0.0	2.4491256746524783	0.0
14	5.514682566599443E-5	0.0	0.0	2.4797321628971054	0.0
15	5.514682566599443E-5	0.0	0.0	2.5370297147640737	0.0
16	5.514682566599443E-5	0.0	0.0	2.670871060655442	0.0
17	5.514682566599443E-5	0.0	0.0	2.837800501946407	0.0
18	5.514682566599443E-5	0.0	0.0	3.0963839674942553	0.0
19	5.514682566599443E-5	0.0	0.0	3.20259675372696	0.0
20	5.514682566599443E-5	0.0	0.0	3.333846198812027	0.0
21	5.514682566599443E-5	0.0	0.0	3.4883676043281433	0.0
22	5.514682566599443E-5	0.0	0.0	3.6375397677546584	0.0
23	5.514682566599443E-5	0.0	0.0	3.808770661447571	0.0
24	5.514682566599443E-5	0.0	0.0	3.9332921938013863	0.0
25	5.514682566599443E-5	0.0	0.0	4.033218241908169	0.0
26	5.514682566599443E-5	0.0	0.0	4.142022928947175	0.0
27	5.514682566599443E-5	0.0	0.0	4.237537231000678	0.0
28	5.514682566599443E-5	0.0	0.0	4.3446875132697045	0.0
29	5.514682566599443E-5	0.0	0.0	4.465569355129564	0.0
30	5.514682566599443E-5	0.0	0.0	4.630072336091226	0.0
31	5.514682566599443E-5	0.0	0.0	4.780733463810723	0.0
32	5.514682566599443E-5	0.0	0.0	4.9083983652275	0.0
33	5.514682566599443E-5	0.0	0.0	5.040419865871891	0.0
34	5.514682566599443E-5	0.0	0.0	5.178783251467871	0.0
35	5.514682566599443E-5	0.0	0.0	5.353267807875077	0.0
36	1.1029365133198885E-4	0.0	0.0	5.502825999081254	0.0
37	1.1029365133198885E-4	0.0	0.0	5.648082737885483	0.0
38	1.1029365133198885E-4	0.0	0.0	5.800232829897962	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTCGA	30	2.528528E-6	44.000004	12
TAGCGAT	30	2.528528E-6	44.000004	14
CGTACCC	30	2.528528E-6	44.000004	37
TAGTGCG	30	2.528528E-6	44.000004	1
TCGACGA	30	2.528528E-6	44.000004	1
ACGCGAT	20	7.857688E-4	44.0	27
CGAACTT	20	7.857688E-4	44.0	25
AAATCCG	25	4.443861E-5	44.0	1
GTCGATC	25	4.443861E-5	44.0	38
TCCGCAT	25	4.443861E-5	44.0	19
GCACGAC	35	1.4466968E-7	44.0	44
TAAGTCG	20	7.857688E-4	44.0	1
CTAAGCG	45	4.802132E-10	44.0	1
ACCTCGC	25	4.443861E-5	44.0	26
CCCGTTA	35	1.4466968E-7	44.0	28
AAACGCG	20	7.857688E-4	44.0	1
CCGTATC	35	1.4466968E-7	44.0	22
TACGCTA	25	4.443861E-5	44.0	1
GCCGAAC	25	4.443861E-5	44.0	35
CTCGGTT	20	7.857688E-4	44.0	24
>>END_MODULE