FastQCFastQC Report
Thu 26 May 2016
SRR1545865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545865_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1061971
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267122.515322923130669No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA19700.18550412393558768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGC408.310963E-944.0000044
CCTCCGC207.856209E-444.00000444
CTAGTCG207.856209E-444.00000419
CTACGCG207.856209E-444.0000041
GTAACGT207.856209E-444.00000431
ACACGAC302.527535E-644.026
GCGCGAC850.044.09
GAGTACG302.527535E-644.01
TAGCGGA254.4426106E-544.03
GCACGCG254.4426106E-544.01
TAACGCG254.4426106E-544.01
TCGACGG254.4426106E-544.02
CGTTTTT189150.043.66271
CGCGACC900.041.55555710
CGACGGT1400.040.85714328
TATTGCG650.040.6153871
TACGGGA5850.040.239324
TCACGTT501.3478711E-939.60000244
TTGTGCG950.039.3684231
CGCTGAA950.039.36842337
Produced by FastQC (version 0.11.5)