##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545865_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1061971
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.452763776035315	31.0	31.0	33.0	30.0	34.0
2	31.840205617667525	31.0	31.0	34.0	30.0	34.0
3	31.671643575954523	31.0	31.0	34.0	30.0	34.0
4	35.56487889028985	37.0	35.0	37.0	33.0	37.0
5	35.557511457469175	37.0	35.0	37.0	33.0	37.0
6	35.62481084700053	37.0	35.0	37.0	33.0	37.0
7	35.98236675012783	37.0	35.0	37.0	35.0	37.0
8	35.98626892824757	37.0	35.0	37.0	35.0	37.0
9	37.77425654749518	39.0	38.0	39.0	35.0	39.0
10	37.23684356729139	39.0	37.0	39.0	33.0	39.0
11	36.99552341824777	39.0	37.0	39.0	33.0	39.0
12	36.81478684446186	39.0	35.0	39.0	33.0	39.0
13	36.71709302796404	39.0	35.0	39.0	33.0	39.0
14	37.93463004168664	40.0	37.0	41.0	33.0	41.0
15	37.98557964388858	40.0	37.0	41.0	33.0	41.0
16	38.049635065364306	40.0	37.0	41.0	33.0	41.0
17	37.94725091363135	40.0	36.0	41.0	33.0	41.0
18	37.63998357770598	39.0	36.0	41.0	33.0	41.0
19	37.332401732250695	38.0	36.0	41.0	33.0	41.0
20	36.941755471665424	38.0	35.0	41.0	32.0	41.0
21	36.83591548168453	38.0	35.0	41.0	32.0	41.0
22	36.7617053573026	38.0	35.0	40.0	32.0	41.0
23	36.640746310398306	38.0	35.0	40.0	32.0	41.0
24	36.63659082969309	38.0	35.0	40.0	32.0	41.0
25	36.61116452332502	38.0	35.0	40.0	32.0	41.0
26	36.36536685088387	38.0	35.0	40.0	31.0	41.0
27	36.36099479175985	38.0	35.0	40.0	31.0	41.0
28	36.32519532077618	38.0	35.0	40.0	31.0	41.0
29	36.219167943380754	38.0	35.0	40.0	31.0	41.0
30	36.00000659151709	38.0	35.0	40.0	30.0	41.0
31	35.69936749685255	38.0	35.0	40.0	29.0	41.0
32	35.20379652551718	38.0	34.0	40.0	25.0	41.0
33	34.53974920219102	38.0	34.0	40.0	21.0	41.0
34	33.89354323234815	38.0	33.0	40.0	16.0	41.0
35	33.35131656137503	38.0	33.0	40.0	14.0	41.0
36	33.05877279134741	38.0	32.0	40.0	10.0	41.0
37	32.93292378040455	38.0	32.0	40.0	10.0	41.0
38	32.810633247047235	38.0	31.0	40.0	10.0	41.0
39	32.60692617783348	37.0	31.0	40.0	10.0	41.0
40	32.52261031610091	37.0	31.0	40.0	10.0	41.0
41	32.41454427663279	37.0	31.0	40.0	9.0	41.0
42	32.35062162714424	37.0	31.0	40.0	9.0	41.0
43	32.26103443502694	37.0	30.0	40.0	8.0	41.0
44	32.1255844086138	37.0	30.0	40.0	8.0	41.0
45	32.041244064103445	37.0	30.0	40.0	8.0	41.0
46	31.945881761366365	37.0	30.0	40.0	8.0	41.0
47	31.88642910211296	37.0	30.0	40.0	8.0	41.0
48	31.751125030721177	36.0	29.0	40.0	8.0	41.0
49	31.661269469693618	36.0	29.0	40.0	8.0	41.0
50	31.564294128559066	36.0	29.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	2.0
14	8.0
15	29.0
16	113.0
17	257.0
18	619.0
19	1358.0
20	2363.0
21	4063.0
22	6118.0
23	9144.0
24	14438.0
25	23966.0
26	37444.0
27	42927.0
28	38015.0
29	32033.0
30	29622.0
31	30697.0
32	34788.0
33	41280.0
34	60282.0
35	88952.0
36	69192.0
37	87677.0
38	139820.0
39	266761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.765420148007806	21.55228344276821	26.137813556114054	25.544482853109923
2	41.20931739190618	22.221510756885074	25.416324927893513	11.152846923315233
3	23.178034051777306	21.398795259004245	43.904682896237276	11.518487792981166
4	20.754333216255436	24.029093073163015	43.2473203128899	11.96925339769165
5	19.345631848703967	28.026565697180057	41.247642355582215	11.380160098533764
6	16.93916312215682	35.89015142598056	38.309426528596354	8.861258923266266
7	75.61882574947903	2.695742162450764	20.54265135300305	1.1427807350671535
8	76.45189934565069	2.21522056628665	20.07229952606992	1.2605805619927475
9	71.32162742673765	5.490922068493396	21.404256801739407	1.7831937030295553
10	26.752425442879325	39.934047163246454	26.366915857401	6.946611536473219
11	22.961078974849595	20.71666740428882	42.76284380646929	13.559409814392295
12	24.95925029967862	19.588199677768976	41.51704707567344	13.935502946878964
13	19.05522843844135	26.2302831244921	39.917568370511056	14.796920066555488
14	15.165668365708667	27.642374415120564	39.97566788546957	17.216289333701205
15	14.044168814402653	24.17740220778157	45.977715022349955	15.800713955465827
16	16.412500906333598	22.463607763300505	43.94931688341772	17.174574446948174
17	16.713544908476784	22.391760226974185	40.84103991540259	20.053654949146445
18	17.39972183797863	22.661824098774826	43.331220909045534	16.60723315420101
19	19.0538159704926	25.0974838295961	38.198689041414504	17.650011158496795
20	21.507837784647606	22.36219256458039	40.1898921910297	15.94007745974231
21	18.227616385004865	24.221753701372258	39.82010808204744	17.730521831575437
22	19.286779017506127	21.8237597825176	38.842680261513735	20.04678093846254
23	15.899963369997863	24.888532737711294	40.311176105562204	18.900327786728642
24	15.497974991784144	22.272265438510093	46.62198873603893	15.607770833666832
25	15.55146044477674	27.53822844503287	40.13292265043019	16.7773884597602
26	17.18907578455532	26.760335263392314	38.23268243671437	17.817906515337988
27	14.946170846473208	26.86306876553126	39.41501227434647	18.775748113649055
28	14.179577408422642	24.788341677880094	42.86152823382183	18.170552679875442
29	15.451457714005373	20.60310498120947	43.27142643254853	20.67401087223662
30	16.183681098636406	26.025004449274036	41.14048312053719	16.650831331552368
31	19.21756808801747	26.168511192866852	37.76440222943941	16.84951848967627
32	19.90892406666472	24.493324205651568	36.18855882128608	19.40919290639763
33	19.51296221836566	27.121456235622254	35.68713269948049	17.67844884653159
34	16.833510519590458	25.34523070780652	36.52133627001114	21.29992250259188
35	17.945781946964654	26.60147970142311	36.171232547781436	19.2815058038308
36	19.20532669912832	29.30701497498519	33.39714549644011	18.09051282944638
37	17.02466451532104	30.853949872454145	34.57071803278997	17.550667579434844
38	17.45188898755239	28.7992798296752	35.84947234905661	17.899358833715798
39	17.74991972473825	31.06920998784336	33.5547769195204	17.626093367897994
40	20.417130034624297	28.787415098905715	32.89402441309603	17.901430453373962
41	18.609171060226693	26.726247703562528	33.64291491952228	21.0216663166885
42	18.357469271759776	28.238435889492276	30.906587844677492	22.49750699407046
43	19.06313825895434	27.769873188627564	32.07884207760852	21.088146474809577
44	19.060595816646593	28.255008846757583	31.948235874614276	20.736159461981543
45	17.53814369695594	31.569788628879696	30.15374242799474	20.738325246169623
46	18.586571573046722	31.26789714596726	30.77155590877717	19.373975372208847
47	18.181852423465426	28.82404510104325	32.69731471010037	20.296787765390956
48	18.84655984014629	26.422755423641515	33.44460441951805	21.286080316694147
49	18.0621693059415	27.456116974945644	33.62869607550489	20.85301764360797
50	17.61535861148751	28.837793122411064	34.084169906711196	19.462678359390228
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	755.5
2	1255.0
3	22458.0
4	43661.0
5	31624.5
6	19588.0
7	19020.0
8	18452.0
9	18791.5
10	19131.0
11	18881.0
12	18631.0
13	18314.5
14	17998.0
15	16988.0
16	15978.0
17	15242.5
18	14507.0
19	13195.5
20	11884.0
21	11326.5
22	10769.0
23	10471.0
24	10173.0
25	11031.5
26	11890.0
27	13396.5
28	14903.0
29	17623.0
30	20343.0
31	23758.0
32	27173.0
33	30976.0
34	34779.0
35	40041.5
36	45304.0
37	47935.0
38	50566.0
39	55396.0
40	60226.0
41	64456.0
42	68686.0
43	76454.0
44	84222.0
45	82924.5
46	81627.0
47	79118.5
48	76610.0
49	72288.0
50	67966.0
51	62256.0
52	56546.0
53	51542.5
54	46539.0
55	39938.5
56	33338.0
57	30216.5
58	27095.0
59	22867.5
60	18640.0
61	15879.5
62	13119.0
63	10804.0
64	8489.0
65	6757.5
66	5026.0
67	3972.0
68	2918.0
69	2157.5
70	1397.0
71	1245.0
72	1093.0
73	784.0
74	475.0
75	401.5
76	328.0
77	236.5
78	145.0
79	138.0
80	131.0
81	97.5
82	64.0
83	47.0
84	30.0
85	22.5
86	15.0
87	9.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1061971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.24226865329947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.19758503880736	26.853757626702812
2	7.608200400709805	5.362604849799112
3	3.575269399807042	3.7800181408776155
4	2.1756533911112075	3.066998452240128
5	1.5397392496078193	2.713195214535425
6	1.1705159512119179	2.475098256934967
7	0.9353743566800333	2.307530005866743
8	0.7303794707508914	2.0592183621646076
9	0.6087004323197158	1.930678574867186
>10	5.041934864910529	35.8113071635772
>50	0.3810278550866144	8.73045532055653
>100	0.033460826029092915	1.8324747828796057
>500	0.0016190722259716783	0.34901564428236226
>1k	2.698453709952797E-4	0.18734627227145875
>5k	0.0	0.0
>10k+	2.698453709952797E-4	2.540301332444267
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26712	2.515322923130669	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	1970	0.18550412393558768	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01902123504314148	0.0
2	0.0	0.0	0.0	0.06516185470224704	0.0
3	0.0	0.0	0.0	0.11139663889126916	0.0
4	0.0	0.0	0.0	0.181737542738926	0.0
5	0.0	0.0	0.0	0.36686500855484755	0.0
6	0.0	0.0	0.0	0.7050098354851497	0.0
7	0.0	0.0	0.0	0.9687646837813838	0.0
8	0.0	0.0	0.0	1.518497209434156	0.0
9	0.0	0.0	0.0	1.8288635000390783	0.0
10	0.0	0.0	0.0	2.1845229295338573	0.0
11	0.0	0.0	0.0	2.385940859025341	0.0
12	0.0	0.0	0.0	2.599694341935891	0.0
13	0.0	0.0	0.0	2.705158615442418	0.0
14	0.0	0.0	0.0	2.7627873077513416	0.0
15	0.0	0.0	0.0	2.820604329120098	0.0
16	0.0	0.0	0.0	2.912038087669061	0.0
17	0.0	0.0	0.0	3.019008993654252	0.0
18	0.0	0.0	0.0	3.1752279487857953	0.0
19	0.0	0.0	0.0	3.268074175283506	0.0
20	0.0	0.0	0.0	3.3787174979354426	0.0
21	0.0	0.0	0.0	3.49152660477546	0.0
22	0.0	0.0	0.0	3.6309842735818587	0.0
23	0.0	0.0	0.0	3.780235053499578	0.0
24	0.0	0.0	0.0	3.8985057030747545	0.0
25	0.0	0.0	0.0	4.002180850512867	0.0
26	0.0	0.0	0.0	4.104820188121898	0.0
27	0.0	0.0	0.0	4.209154487269426	0.0
28	0.0	0.0	0.0	4.33213336334043	0.0
29	0.0	0.0	0.0	4.467541957360417	0.0
30	0.0	0.0	0.0	4.6550235364242525	0.0
31	0.0	0.0	0.0	4.801166886854726	0.0
32	0.0	0.0	0.0	4.947875224464698	0.0
33	0.0	0.0	0.0	5.089122019339511	0.0
34	0.0	0.0	0.0	5.250896681736131	0.0
35	0.0	0.0	0.0	5.446193916783038	0.0
36	0.0	0.0	0.0	5.612582641145568	0.0
37	0.0	0.0	0.0	5.7813254787560115	0.0
38	9.416452991654198E-5	0.0	0.0	5.95807230140936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGC	40	8.310963E-9	44.000004	4
CCTCCGC	20	7.856209E-4	44.000004	44
CTAGTCG	20	7.856209E-4	44.000004	19
CTACGCG	20	7.856209E-4	44.000004	1
GTAACGT	20	7.856209E-4	44.000004	31
ACACGAC	30	2.527535E-6	44.0	26
GCGCGAC	85	0.0	44.0	9
GAGTACG	30	2.527535E-6	44.0	1
TAGCGGA	25	4.4426106E-5	44.0	3
GCACGCG	25	4.4426106E-5	44.0	1
TAACGCG	25	4.4426106E-5	44.0	1
TCGACGG	25	4.4426106E-5	44.0	2
CGTTTTT	18915	0.0	43.6627	1
CGCGACC	90	0.0	41.555557	10
CGACGGT	140	0.0	40.857143	28
TATTGCG	65	0.0	40.615387	1
TACGGGA	585	0.0	40.23932	4
TCACGTT	50	1.3478711E-9	39.600002	44
TTGTGCG	95	0.0	39.368423	1
CGCTGAA	95	0.0	39.368423	37
>>END_MODULE