FastQCFastQC Report
Thu 26 May 2016
SRR1545864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545864_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4660251
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT425310.9126332465783495No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA66800.1433399188155316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT330600.043.0949821
ACGGTCT9950.039.13567730
TCGATAG2650.039.0188671
CGACGGT10150.038.79802728
GCGATCT3050.038.2295079
TTACGTA750.038.1333323
AGGGCGA40600.038.0935946
GACGGTC10450.037.89473729
TATCGCG1800.037.888891
CAATTCG357.294999E-637.7142871
CGGGTAT4950.037.3333326
GTTTTTT399500.037.1989972
TACGGGA24300.037.1193434
TTAGCGG13250.037.0264132
GGGCGAT72350.036.884597
TAGGGCG17650.036.7705355
CGGTCTA10800.036.66666431
CGCCGAT301.3017058E-436.66666441
GACCGAT15450.036.5954679
TAGGGAC52700.036.4857675
Produced by FastQC (version 0.11.5)