##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545864_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4660251
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.435878882918537	31.0	31.0	33.0	30.0	34.0
2	31.840280705910477	31.0	31.0	34.0	30.0	34.0
3	31.90407684049636	31.0	31.0	34.0	30.0	34.0
4	35.680758611499684	37.0	35.0	37.0	33.0	37.0
5	35.605362457944864	37.0	35.0	37.0	33.0	37.0
6	35.657679382505364	37.0	35.0	37.0	33.0	37.0
7	35.979285879666136	37.0	35.0	37.0	35.0	37.0
8	35.96624366369966	37.0	35.0	37.0	35.0	37.0
9	37.74611303125089	39.0	38.0	39.0	35.0	39.0
10	37.1354450650834	39.0	37.0	39.0	33.0	39.0
11	36.89156721386895	39.0	37.0	39.0	32.0	39.0
12	36.55470681729375	39.0	35.0	39.0	32.0	39.0
13	36.39361828365039	39.0	35.0	39.0	32.0	39.0
14	37.474895236329544	40.0	36.0	41.0	32.0	41.0
15	37.562398677667794	40.0	36.0	41.0	32.0	41.0
16	37.640670212827594	40.0	35.0	41.0	33.0	41.0
17	37.557018280775004	40.0	35.0	41.0	32.0	41.0
18	37.421782217309755	39.0	36.0	41.0	32.0	41.0
19	37.298706657645695	39.0	36.0	41.0	32.0	41.0
20	37.13541416546018	39.0	35.0	41.0	32.0	41.0
21	37.023607955880486	39.0	35.0	41.0	32.0	41.0
22	36.945534264141564	39.0	35.0	41.0	32.0	41.0
23	36.83655751589346	38.0	35.0	41.0	31.0	41.0
24	36.89243755325625	38.0	35.0	41.0	32.0	41.0
25	36.88420645154091	38.0	35.0	41.0	32.0	41.0
26	36.656867194492314	38.0	35.0	41.0	31.0	41.0
27	36.62275615626712	38.0	35.0	41.0	31.0	41.0
28	36.592367449736074	38.0	35.0	41.0	31.0	41.0
29	36.52834557623613	38.0	35.0	41.0	31.0	41.0
30	36.396658892407295	38.0	35.0	41.0	30.0	41.0
31	36.222071085870695	38.0	35.0	41.0	30.0	41.0
32	36.02732814176747	38.0	35.0	41.0	30.0	41.0
33	35.75441644666779	38.0	35.0	41.0	28.0	41.0
34	35.45062508435704	38.0	35.0	41.0	26.0	41.0
35	35.19318058190428	38.0	34.0	41.0	24.0	41.0
36	35.029117744945495	38.0	34.0	40.0	24.0	41.0
37	34.958260831873645	38.0	34.0	40.0	23.0	41.0
38	34.86591687872606	38.0	34.0	40.0	23.0	41.0
39	34.757318650862366	38.0	34.0	40.0	23.0	41.0
40	34.66584546626351	38.0	34.0	40.0	23.0	41.0
41	34.60079446364584	38.0	34.0	40.0	23.0	41.0
42	34.5604753907032	38.0	34.0	40.0	23.0	41.0
43	34.4752806232969	38.0	34.0	40.0	22.0	41.0
44	34.35496800494222	38.0	34.0	40.0	22.0	41.0
45	34.2539852467174	37.0	33.0	40.0	22.0	41.0
46	34.19565083511596	37.0	33.0	40.0	22.0	41.0
47	34.13261817872041	37.0	33.0	40.0	22.0	41.0
48	34.0036961528467	37.0	33.0	40.0	20.0	41.0
49	33.939218939065725	37.0	33.0	40.0	20.0	41.0
50	33.81423404018367	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	15.0
12	14.0
13	17.0
14	31.0
15	97.0
16	290.0
17	791.0
18	1904.0
19	3797.0
20	6908.0
21	11229.0
22	17068.0
23	25702.0
24	38359.0
25	59287.0
26	87314.0
27	107555.0
28	110403.0
29	110032.0
30	117611.0
31	134880.0
32	161069.0
33	203339.0
34	322328.0
35	470392.0
36	327052.0
37	426171.0
38	679027.0
39	1237566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.680031826611916	22.744912237559735	29.36775293862927	16.207302997199076
2	33.08647967673844	24.17547896025343	29.857211553626616	12.880829809381511
3	26.709999096615185	23.645250009066036	36.56815909701001	13.07659179730877
4	24.53251981491984	26.118357144282573	35.220806776287375	14.128316264510216
5	22.250561182219585	31.027062705420803	33.168728465483944	13.553647646875671
6	20.019307972896737	39.65543915982207	29.931413565492505	10.393839301788681
7	85.53447013905475	3.073976058371105	10.007615469638868	1.3839383329352861
8	86.65692041051007	2.265993827371101	9.673127048307055	1.4039587138117668
9	81.91389262080519	5.055049609988818	10.866067085227812	2.1649906839781807
10	35.47429097703107	36.016514990287	18.04297665512008	10.466217377561852
11	31.86371292018391	23.438201075435636	29.710202304553984	14.987883699826469
12	30.747721528303945	22.481557323843717	30.30044948222746	16.470271665624878
13	23.309624309935238	28.122626871385254	31.28104044181311	17.286708376866397
14	18.94494524007398	30.8163444415333	31.94029677800616	18.29841354038656
15	17.962852215470797	25.13109272440476	39.77712788431353	17.12892717581092
16	20.46593627682286	23.28082757774206	37.727989329330114	18.52524681610497
17	20.792227714773304	22.812419331061783	32.24776948709415	24.147583467070767
18	22.08765150203283	23.609436487433832	34.883442973350576	19.41946903718276
19	24.45046414881945	25.00914650305316	30.899730508077784	19.640658840049603
20	26.269679465762678	24.836280277607365	30.4282752152191	18.465765041410858
21	23.07021660421295	25.680312069028044	32.44430396560185	18.805167361157157
22	22.85962708875552	22.835594048475073	32.066963775127135	22.237815087642275
23	20.037547333823866	26.687146250277078	31.75472737412641	21.520579041772642
24	19.84204284275675	24.8363446518224	36.3382573170415	18.98335518837934
25	19.941522463060465	25.789297615085538	33.79152753789442	20.477652383959576
26	20.019350889040098	28.751670242654313	30.312294337794256	20.91668453051134
27	19.079058188067552	28.375531704193612	32.67358346149167	19.871826646247165
28	17.645895038700704	26.86140725038201	36.13972723786766	19.352970473049627
29	19.252804194452185	24.038662295228306	35.54083245730756	21.16770105301195
30	20.853769464348595	26.428125867040208	33.63001263236679	19.0880920362444
31	23.79725898883987	25.542250835845536	30.835806912546126	19.824683262768463
32	24.38703408893641	25.458435607867475	30.656224310664808	19.498305992531304
33	24.01615277803706	26.49861563250563	29.951906023945917	19.533325565511387
34	20.021003160559378	27.16815038503291	31.36165841711101	21.449188037296704
35	20.62723660163369	26.55805449105638	31.430045291551895	21.384663615758033
36	24.324634016493963	26.847695542579142	29.29745629580896	19.530214145117935
37	20.93979487370959	29.65956125539161	30.220990242800227	19.179653628098574
38	20.942841919888007	29.10418344419646	29.007107127920793	20.94586750799474
39	20.404630566036037	28.958805008571424	29.022256526526146	21.614307898866393
40	22.27468005478675	27.39564886097337	29.25243726142648	21.077233822813408
41	20.243029828221697	25.88489332441536	29.97495199292914	23.89712485443381
42	21.433158857752513	26.722208739400514	28.381797461123874	23.462834941723095
43	22.06724487586613	27.094441908815643	28.472886975401114	22.36542623991712
44	20.971252406790963	28.04127932165027	28.887907539744106	22.09956073181466
45	19.859788668035264	30.22882243896305	27.898390022339996	22.012998870661686
46	21.283059646358105	29.936391838122024	28.718260025050153	20.06228849046972
47	20.70699625406443	27.622031517186517	29.878197547728654	21.7927746810204
48	20.946468334001754	26.58934035956432	31.006741911540814	21.457449394893107
49	21.465238674912573	25.888541196600784	31.051996984711767	21.594223143774872
50	20.43621684754748	28.381797461123874	29.974758870284024	21.207226821044618
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1226.0
1	3607.5
2	5989.0
3	44557.0
4	83125.0
5	60497.0
6	37869.0
7	37294.0
8	36719.0
9	36645.0
10	36571.0
11	35661.5
12	34752.0
13	33449.5
14	32147.0
15	30288.0
16	28429.0
17	26503.5
18	24578.0
19	23535.0
20	22492.0
21	23008.0
22	23524.0
23	24930.5
24	26337.0
25	30993.5
26	35650.0
27	43963.5
28	52277.0
29	63678.0
30	75079.0
31	88686.0
32	102293.0
33	115784.0
34	129275.0
35	145551.0
36	161827.0
37	173719.0
38	185611.0
39	205350.5
40	225090.0
41	263697.5
42	302305.0
43	339825.5
44	377346.0
45	389119.5
46	400893.0
47	395224.0
48	389555.0
49	382572.0
50	375589.0
51	347003.0
52	318417.0
53	296868.0
54	275319.0
55	253579.0
56	231839.0
57	204268.0
58	176697.0
59	160343.5
60	143990.0
61	126385.5
62	108781.0
63	93191.0
64	77601.0
65	61812.0
66	46023.0
67	37349.0
68	28675.0
69	23131.0
70	17587.0
71	14982.0
72	12377.0
73	9750.0
74	7123.0
75	5662.0
76	4201.0
77	3388.0
78	2575.0
79	1956.0
80	1337.0
81	1000.5
82	664.0
83	473.0
84	282.0
85	195.5
86	109.0
87	82.5
88	56.0
89	39.0
90	22.0
91	15.5
92	9.0
93	9.0
94	9.0
95	8.5
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4660251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.594138275324383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.08769383117009	13.914798211421259
2	8.400971332839925	2.956157025540436
3	2.872360122122736	1.5161010349546513
4	1.4269953220503142	1.0042701205757711
5	0.8494412007383894	0.7472592971274399
6	0.5697597208658317	0.60146587875742
7	0.42414017517813485	0.5223666623143088
8	0.33931527112667165	0.4775967839305475
9	0.27382756646392686	0.4335984061165716
>10	3.0906917814196566	12.901909602996803
>50	1.1955642750190314	15.200088303248597
>100	1.4607846067573351	47.58647566275641
>500	0.007211442152817389	0.8106481369200403
>1k	9.946816762506734E-4	0.25073937300314364
>5k	1.2433520953133417E-4	0.14612973404824633
>10k+	1.2433520953133417E-4	0.930395766288318
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42531	0.9126332465783495	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6680	0.1433399188155316	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03495519876504506	0.0
2	0.0	0.0	0.0	0.13263234104772467	0.0
3	0.0	0.0	0.0	0.18977518592882658	0.0
4	0.0	0.0	0.0	0.2761224663650091	0.0
5	0.0	0.0	0.0	0.48104705089918975	0.0
6	0.0	0.0	0.0	0.670307243107721	0.0
7	0.0	0.0	0.0	0.7972531951605182	0.0
8	0.0	0.0	0.0	1.1281581185219423	0.0
9	0.0	0.0	0.0	1.257786329534611	0.0
10	0.0	0.0	0.0	1.4970438287551464	0.0
11	0.0	0.0	0.0	1.7420950073290045	0.0
12	0.0	0.0	0.0	1.9497876831097725	0.0
13	0.0	0.0	0.0	2.026993825010713	0.0
14	0.0	0.0	0.0	2.0574642867948527	0.0
15	0.0	0.0	0.0	2.109156781469496	0.0
16	0.0	0.0	0.0	2.232583609766942	0.0
17	0.0	0.0	0.0	2.3821678274410543	0.0
18	0.0	0.0	0.0	2.582478926564256	0.0
19	0.0	0.0	0.0	2.6848553865446303	0.0
20	0.0	0.0	0.0	2.7980896307945646	0.0
21	0.0	0.0	0.0	2.9534246116786416	0.0
22	0.0	0.0	0.0	3.1273637407083865	0.0
23	0.0	0.0	0.0	3.3048863677085203	0.0
24	0.0	0.0	0.0	3.4471104667967456	0.0
25	0.0	0.0	0.0	3.57015104980397	0.0
26	0.0	0.0	0.0	3.6898656317009535	0.0
27	0.0	0.0	0.0	3.815845970528197	0.0
28	0.0	0.0	0.0	3.9523193064064577	0.0
29	0.0	0.0	0.0	4.102397059729186	0.0
30	0.0	0.0	0.0	4.2765078533323635	0.0
31	0.0	0.0	0.0	4.44164917297373	0.0
32	0.0	0.0	0.0	4.595181675836773	0.0
33	0.0	0.0	0.0	4.7463752488868085	0.0
34	0.0	0.0	0.0	4.913276130405851	0.0
35	0.0	0.0	0.0	5.1073858468138305	0.0
36	0.0	0.0	0.0	5.278363761951877	0.0
37	0.0	0.0	0.0	5.459577177280795	0.0
38	0.0	0.0	0.0	5.648236543482315	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	33060	0.0	43.094982	1
ACGGTCT	995	0.0	39.135677	30
TCGATAG	265	0.0	39.018867	1
CGACGGT	1015	0.0	38.798027	28
GCGATCT	305	0.0	38.229507	9
TTACGTA	75	0.0	38.13333	23
AGGGCGA	4060	0.0	38.093594	6
GACGGTC	1045	0.0	37.894737	29
TATCGCG	180	0.0	37.88889	1
CAATTCG	35	7.294999E-6	37.714287	1
CGGGTAT	495	0.0	37.333332	6
GTTTTTT	39950	0.0	37.198997	2
TACGGGA	2430	0.0	37.119343	4
TTAGCGG	1325	0.0	37.026413	2
GGGCGAT	7235	0.0	36.88459	7
TAGGGCG	1765	0.0	36.770535	5
CGGTCTA	1080	0.0	36.666664	31
CGCCGAT	30	1.3017058E-4	36.666664	41
GACCGAT	1545	0.0	36.595467	9
TAGGGAC	5270	0.0	36.485767	5
>>END_MODULE