##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545863_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1725206
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.491801558770373	31.0	31.0	33.0	30.0	34.0
2	31.91191602626005	31.0	31.0	34.0	30.0	34.0
3	32.02643336505901	33.0	31.0	34.0	30.0	34.0
4	35.778389363357185	37.0	35.0	37.0	35.0	37.0
5	35.7022709172122	37.0	35.0	37.0	33.0	37.0
6	35.75153923647379	37.0	35.0	37.0	33.0	37.0
7	35.96126491561008	37.0	35.0	37.0	35.0	37.0
8	35.93414641497885	37.0	35.0	37.0	35.0	37.0
9	37.697728850931426	39.0	37.0	39.0	35.0	39.0
10	37.27449417634764	39.0	37.0	39.0	34.0	39.0
11	37.05032268610241	39.0	37.0	39.0	33.0	39.0
12	36.54860926753095	39.0	35.0	39.0	33.0	39.0
13	36.34486606237168	39.0	35.0	39.0	32.0	39.0
14	37.440807068836996	40.0	35.0	41.0	33.0	41.0
15	37.54910891800747	40.0	35.0	41.0	33.0	41.0
16	37.645027898117675	40.0	35.0	41.0	33.0	41.0
17	37.58105524789504	39.0	35.0	41.0	33.0	41.0
18	37.47500472407353	39.0	36.0	41.0	33.0	41.0
19	37.3867138185237	39.0	36.0	41.0	32.0	41.0
20	37.22952911130613	39.0	35.0	41.0	32.0	41.0
21	37.126798770697526	39.0	35.0	41.0	32.0	41.0
22	37.021950422152486	39.0	35.0	41.0	32.0	41.0
23	36.90435808825149	38.0	35.0	41.0	32.0	41.0
24	36.97662713901992	39.0	35.0	41.0	32.0	41.0
25	36.93420264014848	38.0	35.0	41.0	32.0	41.0
26	36.704010999266174	38.0	35.0	41.0	31.0	41.0
27	36.68815666071182	38.0	35.0	41.0	31.0	41.0
28	36.669026191654794	38.0	35.0	41.0	31.0	41.0
29	36.63361013119593	38.0	35.0	41.0	31.0	41.0
30	36.54331888481723	38.0	35.0	41.0	31.0	41.0
31	36.409298367847086	38.0	35.0	41.0	31.0	41.0
32	36.23851586419245	38.0	35.0	41.0	30.0	41.0
33	36.02004456279424	38.0	35.0	41.0	30.0	41.0
34	35.801391254145884	38.0	35.0	41.0	29.0	41.0
35	35.57578689153643	38.0	35.0	41.0	27.0	41.0
36	35.43220287896054	38.0	35.0	40.0	27.0	41.0
37	35.3538841158679	38.0	34.0	40.0	26.0	41.0
38	35.28255466303734	38.0	34.0	40.0	26.0	41.0
39	35.181091417488695	38.0	34.0	40.0	26.0	41.0
40	35.037441905488386	38.0	34.0	40.0	25.0	41.0
41	35.00219857802489	38.0	34.0	40.0	24.0	41.0
42	34.979636055056616	38.0	34.0	40.0	24.0	41.0
43	34.89545074617176	38.0	34.0	40.0	24.0	41.0
44	34.78988538180368	37.0	34.0	40.0	24.0	41.0
45	34.69090184012808	37.0	34.0	40.0	23.0	41.0
46	34.627413190077014	37.0	34.0	40.0	23.0	41.0
47	34.55671496621273	37.0	34.0	40.0	23.0	41.0
48	34.45425241971104	37.0	34.0	40.0	23.0	41.0
49	34.37474191487857	37.0	34.0	40.0	24.0	41.0
50	34.240635611051665	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	1.0
12	7.0
13	8.0
14	16.0
15	34.0
16	99.0
17	305.0
18	624.0
19	1207.0
20	2049.0
21	3530.0
22	5454.0
23	7910.0
24	11559.0
25	17737.0
26	25855.0
27	32827.0
28	35987.0
29	37094.0
30	40723.0
31	47620.0
32	58230.0
33	76097.0
34	130013.0
35	198758.0
36	120567.0
37	156587.0
38	250941.0
39	463365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.46139881266353	23.033713075424036	28.868784365461288	15.63610374645115
2	32.0216252435941	25.085526018342158	30.294527146323396	12.59832159174035
3	29.448831038148487	23.82260437304299	33.526662902864935	13.201901685943593
4	26.537932281710123	27.293842010751177	32.343615776898524	13.824609930640166
5	23.041886012453006	32.36633770112091	31.27197563653268	13.319800649893404
6	21.15335791783706	40.4990476499618	27.95967554019636	10.387918892004782
7	86.01453971293863	4.085483124913778	8.235596212858058	1.664380949289534
8	86.99575586915418	3.4049846800903776	8.116595931152569	1.4826635196028763
9	80.86941501478664	6.54889908799297	9.730490155958186	2.851195741262203
10	38.06026642615433	37.584033442962756	14.173553766912473	10.182146363970448
11	37.55481954039112	21.625011737728713	25.52002485500282	15.300143866877347
12	35.735152787551165	20.725756808172473	27.552767611520018	15.986322792756344
13	23.020787082817936	31.175175602217937	28.271870141884502	17.53216717307962
14	17.220957960962345	34.037906197868544	30.62161851975938	18.119517321409734
15	17.435367138764878	23.932678184518256	40.99898794694663	17.632966729770242
16	19.622062524707196	21.653356178914287	39.65665549505392	19.067925801324595
17	19.427650958784053	21.876402006485023	30.696218306683377	27.99972872804755
18	21.55922249285013	23.641988261112008	33.521504098640975	21.277285147396892
19	26.087725176007964	24.409896557280696	28.73923461893826	20.763143647773077
20	27.915622829969287	23.605239026527848	29.134665657318603	19.34447248618426
21	24.20696426977416	25.33030838056441	29.551601373980844	20.911125975680587
22	23.24307937718742	23.617585378209906	29.164806985368703	23.97452825923397
23	21.448800896820437	26.808740521421793	29.40193808739362	22.340520494364153
24	20.675212119596154	23.44351920872058	35.680898397060986	20.200370274622276
25	19.599862277316447	25.429137158113292	33.40882190300752	21.562178661562736
26	20.08415226935218	29.97960823229226	28.979032069213762	20.957207429141793
27	19.7901004285865	28.96767110710257	30.591998868540916	20.65022959577001
28	17.88980562321253	27.17727622092666	35.68565145263812	19.24726670322269
29	19.464863906107443	24.188357796112463	34.538136315315384	21.808641982464703
30	20.286041203195445	27.90982642072889	31.257774433893694	20.546357942181977
31	25.130390225862882	25.812859449828018	28.603656606805217	20.453093717503883
32	25.868215158073877	26.157629871447234	29.044821314092346	18.92933365638654
33	24.951918785350852	27.163248910564885	27.810186145886345	20.074646158197922
34	20.481785943243878	26.717505039977834	30.293483792660126	22.507225224118162
35	21.873387873680013	25.09677105226854	31.012180574377783	22.01766049967366
36	25.307818312711643	26.893078275869662	27.36334095754362	20.43576245387507
37	21.46763922685175	30.44135019238282	28.194719934894728	19.896290645870696
38	20.921559512313312	29.910225213684626	27.058449831498383	22.10976544250368
39	22.603155797046846	29.14880889586519	27.153684835318217	21.094350471769747
40	23.030930798988642	27.746077859687478	27.17739214911147	22.04559919221241
41	19.38284471535573	26.80108926122446	28.68161831108865	25.134447712331166
42	21.549542489418656	26.930696971839886	27.12789081419842	24.39186972454304
43	22.027572359474753	26.80520471178514	27.552187970595977	23.61503495814413
44	21.06797680972591	28.595889418423077	28.345600467422443	21.990533304428574
45	19.79618665828892	32.039478184054545	25.79349944296507	22.370835714691463
46	21.30557162449006	30.433467075815873	27.24521013722419	21.015751162469872
47	21.80128054272939	26.905192771182108	28.87463873879409	22.41888794729441
48	21.707204820757635	25.55399181315159	30.70271028503263	22.036093081058148
49	20.97790061013004	26.09050745244336	30.662367276719415	22.269224660707184
50	19.827371340002294	29.074151144848788	28.889477546449527	22.208999968699388
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	650.0
1	1650.5
2	2651.0
3	14331.5
4	26012.0
5	18912.5
6	11813.0
7	11387.0
8	10961.0
9	10837.5
10	10714.0
11	10336.0
12	9958.0
13	9396.0
14	8834.0
15	8048.5
16	7263.0
17	6897.0
18	6531.0
19	6489.0
20	6447.0
21	6679.0
22	6911.0
23	7821.0
24	8731.0
25	11765.0
26	14799.0
27	18400.0
28	22001.0
29	27858.0
30	33715.0
31	38150.5
32	42586.0
33	45036.5
34	47487.0
35	52233.5
36	56980.0
37	59793.0
38	62606.0
39	70480.5
40	78355.0
41	95590.0
42	112825.0
43	125833.0
44	138841.0
45	139736.0
46	140631.0
47	131192.5
48	121754.0
49	123751.0
50	125748.0
51	118915.5
52	112083.0
53	103716.0
54	95349.0
55	90098.0
56	84847.0
57	79645.0
58	74443.0
59	71557.5
60	68672.0
61	63278.5
62	57885.0
63	50079.0
64	42273.0
65	33394.0
66	24515.0
67	20849.5
68	17184.0
69	15166.5
70	13149.0
71	10830.5
72	8512.0
73	6735.0
74	4958.0
75	3842.0
76	2726.0
77	2191.0
78	1656.0
79	1159.0
80	662.0
81	459.5
82	257.0
83	190.5
84	124.0
85	95.0
86	66.0
87	43.0
88	20.0
89	16.5
90	13.0
91	7.5
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1725206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.935481731591327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.10271910418953	13.396426543082022
2	6.760263081931391	2.289766238496007
3	2.202103471422342	1.1188104933404077
4	1.1705778727142901	0.7929720071503159
5	0.7383987426744999	0.6252569208597
6	0.5658327895936531	0.5749590522779202
7	0.46032609897211624	0.5457090967801883
8	0.38269632396768294	0.518491728264148
9	0.32566138935945105	0.49637092591634696
>10	4.912351614507483	20.928326473754417
>50	2.0380479266962195	24.85848730321222
>100	1.335129268921623	31.90278138263859
>500	0.003119460908695281	0.3412790430762795
>1k	0.0024262473734296634	0.8226151547421708
>5k	0.0	0.0
>10k+	3.4660676763280905E-4	0.7877476364093002
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13420	0.7778781200621839	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3246	0.18815144394350589	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2724	0.15789418770859828	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2688	0.15580748038205292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0325758199310691	0.0
2	0.0	0.0	0.0	0.09181512236799548	0.0
3	0.0	0.0	0.0	0.12821657239773104	0.0
4	0.0	0.0	0.0	0.17360245675009245	0.0
5	0.0	0.0	0.0	0.2828647709317032	0.0
6	0.0	0.0	0.0	0.39015630597157674	0.0
7	0.0	0.0	0.0	0.46475609289557307	0.0
8	0.0	0.0	0.0	0.6641525707654622	0.0
9	0.0	0.0	0.0	0.751794278480367	0.0
10	0.0	0.0	0.0	0.9167600854622578	0.0
11	0.0	0.0	0.0	1.0716981044582503	0.0
12	0.0	0.0	0.0	1.2284330103187677	0.0
13	5.796409240403755E-5	0.0	0.0	1.287498420478482	0.0
14	5.796409240403755E-5	0.0	0.0	1.3086553142059556	0.0
15	5.796409240403755E-5	0.0	0.0	1.34604215380656	0.0
16	5.796409240403755E-5	0.0	0.0	1.4245255349216268	0.0
17	5.796409240403755E-5	0.0	0.0	1.5208038924047331	0.0
18	5.796409240403755E-5	0.0	0.0	1.6614827446693323	0.0
19	5.796409240403755E-5	0.0	0.0	1.725938815422622	0.0
20	5.796409240403755E-5	0.0	0.0	1.8053496220161533	0.0
21	5.796409240403755E-5	0.0	0.0	1.900526661743583	0.0
22	5.796409240403755E-5	0.0	0.0	2.006542986750568	0.0
23	5.796409240403755E-5	0.0	0.0	2.1144141627144815	0.0
24	5.796409240403755E-5	0.0	0.0	2.199737306733225	0.0
25	5.796409240403755E-5	0.0	0.0	2.2701057149117263	0.0
26	5.796409240403755E-5	0.0	0.0	2.3402422667206118	0.0
27	5.796409240403755E-5	0.0	0.0	2.4106106748991136	0.0
28	5.796409240403755E-5	0.0	0.0	2.4879927382585034	0.0
29	5.796409240403755E-5	0.0	0.0	2.567229652574823	0.0
30	5.796409240403755E-5	0.0	0.0	2.675274720815949	0.0
31	5.796409240403755E-5	0.0	0.0	2.777117631169843	0.0
32	5.796409240403755E-5	0.0	0.0	2.8706716763099593	0.0
33	5.796409240403755E-5	0.0	0.0	2.9601102708893894	0.0
34	5.796409240403755E-5	0.0	0.0	3.0558669515408594	0.0
35	5.796409240403755E-5	0.0	0.0	3.1734181309362475	0.0
36	5.796409240403755E-5	0.0	0.0	3.291259130793656	0.0
37	5.796409240403755E-5	0.0	0.0	3.398086953094297	0.0
38	5.796409240403755E-5	0.0	0.0	3.5050307035797466	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAC	30	2.5284571E-6	44.0	14
CGTCTAC	20	7.85758E-4	44.0	38
CGCGAAA	25	4.443771E-5	44.0	32
TATAGCG	155	0.0	44.0	1
CGCACTA	30	2.5284571E-6	44.0	28
TGCGTCG	25	4.443771E-5	44.0	1
CGTTTTT	8680	0.0	42.758064	1
GCGTTAG	95	0.0	41.68421	1
CGTTAGG	240	0.0	41.25	2
CGGTCTA	230	0.0	41.130436	31
TCTTACG	70	0.0	40.857143	1
CGTTAAG	70	0.0	40.857143	16
TACGTCT	70	0.0	40.857143	44
ACGGTCT	235	0.0	40.255318	30
ACGGTAC	55	7.8216544E-11	40.0	13
ATCGAGA	50	1.3478711E-9	39.6	23
TACGGGA	690	0.0	39.536232	4
TGCGTAG	140	0.0	39.285713	1
AACGGTC	45	2.3521352E-8	39.11111	28
TATGACG	85	0.0	38.82353	1
>>END_MODULE