FastQCFastQC Report
Thu 26 May 2016
SRR1545861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545861_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2857386
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387751.3570095184899764No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA60170.2105770798905013No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA254.4445063E-544.012
CGTCTAC207.8584487E-444.036
CGCGAGT254.4445063E-544.027
AATACCG207.8584487E-444.01
CGTTTTT259650.043.288271
ACCGCTC1650.041.33333218
CGTAAGG6550.040.97712
TATAGCG2450.040.408161
ACGGGTA5350.039.8878485
ACGGGAT7650.039.6862755
GCGATAT1000.039.6000029
AGTACGG4000.039.6000022
TACGGGT5400.039.111114
TACGATA452.3530447E-839.1111134
ATTAGCG1750.038.9714281
CGACGGT4350.038.94252828
GCGCGAC5100.038.8235329
TAATGCG1250.038.721
CGTATGG3250.038.5846142
TAGTCCG404.1274143E-738.51
Produced by FastQC (version 0.11.5)