##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545861_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2857386
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.402505996739677	31.0	31.0	33.0	30.0	34.0
2	31.79388679023415	31.0	31.0	34.0	30.0	34.0
3	31.837156058019463	31.0	31.0	34.0	30.0	34.0
4	35.63300968087616	37.0	35.0	37.0	33.0	37.0
5	35.574286078254744	37.0	35.0	37.0	33.0	37.0
6	35.624731485350594	37.0	35.0	37.0	33.0	37.0
7	35.96064759888934	37.0	35.0	37.0	35.0	37.0
8	35.95591705145892	37.0	35.0	37.0	35.0	37.0
9	37.74470407568316	39.0	38.0	39.0	35.0	39.0
10	36.99861306802791	39.0	37.0	39.0	33.0	39.0
11	36.81607070238323	39.0	35.0	39.0	32.0	39.0
12	36.32704191873272	38.0	35.0	39.0	32.0	39.0
13	36.04024552510581	38.0	35.0	39.0	32.0	39.0
14	36.99550988210903	39.0	35.0	41.0	31.0	41.0
15	37.1791557038496	39.0	35.0	41.0	32.0	41.0
16	37.334653420993874	39.0	35.0	41.0	32.0	41.0
17	37.28455518435381	39.0	35.0	41.0	32.0	41.0
18	37.15225174337664	39.0	35.0	40.0	32.0	41.0
19	36.974375180672126	38.0	35.0	40.0	32.0	41.0
20	36.74151129738859	38.0	35.0	40.0	32.0	41.0
21	36.58200817110464	38.0	35.0	40.0	31.0	41.0
22	36.52322472357602	38.0	35.0	40.0	31.0	41.0
23	36.464498671163085	38.0	35.0	40.0	31.0	41.0
24	36.50969242517462	38.0	35.0	40.0	31.0	41.0
25	36.490862627590396	38.0	35.0	40.0	31.0	41.0
26	36.25935417895937	38.0	35.0	40.0	31.0	41.0
27	36.242852733232404	38.0	35.0	40.0	31.0	41.0
28	36.22897151452411	38.0	35.0	40.0	31.0	41.0
29	36.192512667172025	38.0	35.0	40.0	31.0	41.0
30	36.039352751080884	38.0	35.0	40.0	30.0	41.0
31	35.83410466769278	38.0	35.0	40.0	30.0	41.0
32	35.569932098778395	38.0	35.0	40.0	29.0	41.0
33	35.22330759652353	38.0	34.0	40.0	27.0	41.0
34	34.878352802176536	38.0	34.0	40.0	24.0	41.0
35	34.596025878197764	37.0	34.0	40.0	23.0	41.0
36	34.396567702088554	37.0	34.0	40.0	22.0	41.0
37	34.28768496800922	37.0	33.0	40.0	21.0	41.0
38	34.19063787671669	37.0	33.0	40.0	21.0	41.0
39	34.07531534066451	37.0	33.0	40.0	21.0	41.0
40	33.954246993580846	37.0	33.0	40.0	19.0	41.0
41	33.89345786673554	37.0	33.0	40.0	18.0	41.0
42	33.87244810466629	37.0	33.0	40.0	18.0	41.0
43	33.77739864337545	37.0	33.0	40.0	18.0	41.0
44	33.665695849283225	36.0	33.0	40.0	18.0	41.0
45	33.56720128117097	36.0	33.0	40.0	18.0	41.0
46	33.52275611345475	36.0	33.0	40.0	18.0	41.0
47	33.463359168134794	36.0	33.0	40.0	18.0	41.0
48	33.355669482527034	36.0	33.0	40.0	18.0	41.0
49	33.30595481324539	36.0	33.0	40.0	18.0	41.0
50	33.138988922042735	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	6.0
14	19.0
15	65.0
16	194.0
17	543.0
18	1379.0
19	2686.0
20	4664.0
21	7768.0
22	11926.0
23	17720.0
24	26913.0
25	41800.0
26	62141.0
27	76835.0
28	77990.0
29	75545.0
30	78994.0
31	89193.0
32	105816.0
33	134271.0
34	223346.0
35	351273.0
36	186841.0
37	238066.0
38	377484.0
39	663906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.175692048606663	21.535907294289256	29.658401070068933	18.629999587035144
2	34.09626840755852	23.815368312156636	29.666975340398533	12.421387939886316
3	26.82826191491104	23.73372026040584	36.9212280034969	12.516789821186217
4	23.56912926709937	25.520388214962907	37.279912479447994	13.630570038489726
5	21.629454333436225	30.798184074535257	35.27892276367281	12.293438828355708
6	19.202340880791045	38.03840293191049	31.981713356193385	10.777542831105073
7	83.23999627631689	3.306028656961293	12.024241737028179	1.4297333296936432
8	84.95761510695439	1.804271456499052	11.64676386039548	1.5913495761510694
9	80.12480637897715	3.5623468442835518	13.77451278896166	2.5383339877776403
10	40.53628036254115	22.31711081386974	24.616835107332367	12.529773716256745
11	37.10849706689961	18.773942337507076	27.411592273497526	16.705968322095792
12	34.342052491332986	20.871908800561073	28.249036007035798	16.53700270107014
13	23.146155262187186	30.87066290658665	29.718980914724156	16.264200916502006
14	17.569904801101426	33.265544102196905	32.705941724359256	16.458609372342416
15	16.56867500575701	22.606606177814268	45.01708904572221	15.807629770706514
16	18.58807315497451	19.514969276114602	42.93378633478291	18.963171234127977
17	19.18960196487279	19.391324798259667	33.745213282349674	27.67385995451787
18	21.702493117835672	21.45719899236575	36.96714409603743	19.87316379376115
19	24.861674271519494	22.85795478804754	31.928132915888856	20.35223802454411
20	25.830496824720218	23.260875499494993	31.512543282566654	19.39608439321814
21	21.062327595921587	24.514503815725284	34.75519233313245	19.667976255220683
22	20.539507087946816	21.608141147188377	33.96485459087432	23.887497173990493
23	18.356567856075447	25.302601748591197	33.3030609095166	23.037769485816757
24	18.359752585055013	23.63985824806309	39.93111186238051	18.069277304501387
25	17.96708600098132	23.824222558660257	37.57007278680584	20.63861865355258
26	18.079111467614105	28.647092132459527	32.92901274101574	20.344783658910625
27	17.992738817926597	28.03971881992842	34.43437463471859	19.533167727426395
28	15.913915725771737	25.55930490315274	39.37724899611043	19.14953037496509
29	17.042814656472736	22.935963149535972	38.39047297075019	21.630749223241104
30	18.826577858224265	25.8532098918382	36.068245592300094	19.251966657637436
31	24.05898258058239	24.599931545825452	31.441814301602932	19.899271571989225
32	25.065041964928785	24.25734569988094	31.88788634087239	18.789725994317884
33	23.820757853506667	25.136785859523354	31.181086489539737	19.86136979743024
34	18.706958037870976	26.319335224572388	32.653096221511554	22.320610516045083
35	19.476332564098794	25.830986783024763	32.72728990762886	21.96539074524758
36	24.918019476542547	24.578548365534093	30.76882157328411	19.73461058463925
37	19.69495895899259	29.22023835771576	31.09208906322072	19.99271362007093
38	20.06515745510057	29.598591159892294	28.918809009353303	21.41744237565383
39	19.982004531414375	28.439874766657358	30.318619885447752	21.259500816480518
40	21.730420741194926	26.16699318887963	29.477501464625362	22.62508460530009
41	18.53032806908132	24.79143524886032	30.19879008296394	26.47944659909442
42	21.304646974542464	25.587582496729528	28.39899824524933	24.708772283478673
43	20.953941819551154	25.995787758461752	29.51820300092462	23.532067421062468
44	19.81863843386928	28.19916525103714	29.999762020252074	21.98243429484151
45	18.438110916760984	31.36762061548562	27.71627634488305	22.477992122870344
46	20.85857493527301	28.63424822547601	29.723495530530354	20.783681308720627
47	20.513049339501208	27.094659244498292	30.127676134760932	22.264615281239568
48	21.13085176451484	25.160233864098164	31.414026666330695	22.294887705056297
49	20.37152138353026	23.90867037215133	33.090384008320896	22.629424235997515
50	19.114253377037613	28.5423110493297	30.474111653098323	21.869323920534363
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	713.0
1	2189.5
2	3666.0
3	34816.0
4	65966.0
5	46923.0
6	27880.0
7	27709.0
8	27538.0
9	28356.5
10	29175.0
11	28355.0
12	27535.0
13	26162.5
14	24790.0
15	22661.0
16	20532.0
17	19145.0
18	17758.0
19	16381.5
20	15005.0
21	14427.5
22	13850.0
23	14644.0
24	15438.0
25	16141.5
26	16845.0
27	19999.0
28	23153.0
29	27606.0
30	32059.0
31	37969.0
32	43879.0
33	50677.5
34	57476.0
35	65920.5
36	74365.0
37	82079.0
38	89793.0
39	107168.5
40	124544.0
41	155085.0
42	185626.0
43	216169.0
44	246712.0
45	262525.0
46	278338.0
47	269158.0
48	259978.0
49	254053.0
50	248128.0
51	229559.5
52	210991.0
53	192972.5
54	174954.0
55	158815.5
56	142677.0
57	125840.5
58	109004.0
59	98525.5
60	88047.0
61	74037.5
62	60028.0
63	50741.0
64	41454.0
65	33235.0
66	25016.0
67	19685.0
68	14354.0
69	10975.0
70	7596.0
71	6227.0
72	4858.0
73	4228.0
74	3598.0
75	2726.5
76	1855.0
77	1461.0
78	1067.0
79	836.5
80	606.0
81	442.0
82	278.0
83	212.5
84	147.0
85	105.5
86	64.0
87	48.5
88	33.0
89	21.5
90	10.0
91	8.0
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2857386.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.72753567047777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52193424175645	15.68771794339638
2	8.474307322156129	3.343543999608519
3	2.908499649606388	1.7213259175554634
4	1.414691204861782	1.1163348482648792
5	0.836463993505291	0.8250686634472957
6	0.5403460559814541	0.6395817656265668
7	0.41106836627179894	0.5676556101042354
8	0.3252266255137563	0.5132735884649394
9	0.24728829977713143	0.4390549879270645
>10	2.9842311357933116	13.839259533177767
>50	1.0975400685158219	15.627906502802638
>100	1.2308386259636006	43.092103806453494
>500	0.006483780254709589	0.7981521990269747
>1k	7.204200283010654E-4	0.19754835410389862
>5k	1.8010500707526636E-4	0.21378569184229462
>10k+	1.8010500707526636E-4	1.3776865881976024
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38775	1.3570095184899764	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6017	0.2105770798905013	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07800836148843733	0.0
2	0.0	0.0	0.0	0.281201069788961	0.0
3	0.0	0.0	0.0	0.3883969474197746	0.0
4	0.0	0.0	0.0	0.5465134917018561	0.0
5	0.0	0.0	0.0	0.9150671277874253	0.0
6	0.0	0.0	0.0	1.2453690190964748	0.0
7	0.0	0.0	0.0	1.4771892911913196	0.0
8	0.0	0.0	0.0	2.0330469877013466	0.0
9	0.0	0.0	0.0	2.219511119603722	0.0
10	0.0	0.0	0.0	2.554222635653706	0.0
11	0.0	0.0	0.0	2.893378773466378	0.0
12	3.4997021753448784E-5	0.0	0.0	3.185358925955401	0.0
13	3.4997021753448784E-5	0.0	0.0	3.2952145772394767	0.0
14	3.4997021753448784E-5	0.0	0.0	3.335391158212436	0.0
15	3.4997021753448784E-5	0.0	0.0	3.3997506812170286	0.0
16	3.4997021753448784E-5	0.0	0.0	3.5629767906751137	0.0
17	3.4997021753448784E-5	0.0	0.0	3.7651195883230337	0.0
18	3.4997021753448784E-5	0.0	0.0	4.0350866141291375	0.0
19	3.4997021753448784E-5	0.0	0.0	4.169755153836409	0.0
20	3.4997021753448784E-5	0.0	0.0	4.316602657113879	0.0
21	3.4997021753448784E-5	0.0	0.0	4.522070171828378	0.0
22	3.4997021753448784E-5	0.0	0.0	4.75021575663911	0.0
23	3.4997021753448784E-5	0.0	0.0	4.989420400323932	0.0
24	3.4997021753448784E-5	0.0	0.0	5.1788592790753505	0.0
25	3.4997021753448784E-5	0.0	0.0	5.34649501327437	0.0
26	3.4997021753448784E-5	0.0	0.0	5.510421063167525	0.0
27	3.4997021753448784E-5	0.0	0.0	5.679351687171422	0.0
28	3.4997021753448784E-5	0.0	0.0	5.84331273408633	0.0
29	3.4997021753448784E-5	0.0	0.0	6.037301225665696	0.0
30	3.4997021753448784E-5	0.0	0.0	6.258972361452041	0.0
31	3.4997021753448784E-5	0.0	0.0	6.452365903661598	0.0
32	3.4997021753448784E-5	0.0	0.0	6.634665389975313	0.0
33	3.4997021753448784E-5	0.0	0.0	6.818994703550728	0.0
34	3.4997021753448784E-5	0.0	0.0	7.01648289730544	0.0
35	3.4997021753448784E-5	0.0	0.0	7.239868887157703	0.0
36	3.4997021753448784E-5	0.0	0.0	7.44106676521828	0.0
37	3.4997021753448784E-5	0.0	0.0	7.6359301823414825	0.0
38	3.4997021753448784E-5	0.0	0.0	7.86848539189315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	4.4445063E-5	44.0	12
CGTCTAC	20	7.8584487E-4	44.0	36
CGCGAGT	25	4.4445063E-5	44.0	27
AATACCG	20	7.8584487E-4	44.0	1
CGTTTTT	25965	0.0	43.28827	1
ACCGCTC	165	0.0	41.333332	18
CGTAAGG	655	0.0	40.9771	2
TATAGCG	245	0.0	40.40816	1
ACGGGTA	535	0.0	39.887848	5
ACGGGAT	765	0.0	39.686275	5
GCGATAT	100	0.0	39.600002	9
AGTACGG	400	0.0	39.600002	2
TACGGGT	540	0.0	39.11111	4
TACGATA	45	2.3530447E-8	39.11111	34
ATTAGCG	175	0.0	38.971428	1
CGACGGT	435	0.0	38.942528	28
GCGCGAC	510	0.0	38.823532	9
TAATGCG	125	0.0	38.72	1
CGTATGG	325	0.0	38.584614	2
TAGTCCG	40	4.1274143E-7	38.5	1
>>END_MODULE