FastQCFastQC Report
Thu 26 May 2016
SRR1545860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545860_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4727405
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473891.0024315665782813No Hit
GGCTTCCCTTCGACTCTTCCTCCTTTGCCTCGCTGGACTGGTATTTGTGT106330.2249225526478057No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA103970.21993038464019904No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA61980.1311078699624847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT207.858972E-444.032
CCACGTA207.858972E-444.014
ACGCGCT254.4449516E-544.034
CGTTTTT316900.043.01421
CTATGCG1000.041.81
CGCATCG750.041.06666621
TCGGGTA1300.040.6153875
TCGATAG2450.040.408161
CCGCTCG2850.040.1403519
TACGGGA22700.039.348024
TAGGGAC82650.039.315185
ACGGGAC30550.039.1751255
CGGTCTA6800.039.1470631
ATGCGAA452.3535904E-839.1111132
TCGACCA452.3535904E-839.1111124
ATAGGGA79350.039.0371744
GTAGGGC15900.039.0188674
TAGGGTA16900.038.9230775
AGGGACA191050.038.898726
TGGGCGA19750.038.7645576
Produced by FastQC (version 0.11.5)