##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545860_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4727405
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.546230966037392	31.0	31.0	33.0	30.0	34.0
2	31.97000151245768	31.0	31.0	34.0	30.0	34.0
3	32.04442775687719	33.0	31.0	34.0	30.0	34.0
4	35.80439226171652	37.0	35.0	37.0	35.0	37.0
5	35.74464552962989	37.0	35.0	37.0	33.0	37.0
6	35.78453950951949	37.0	35.0	37.0	33.0	37.0
7	36.013899380315415	37.0	35.0	37.0	35.0	37.0
8	35.999761602824385	37.0	35.0	37.0	35.0	37.0
9	37.825902794450656	39.0	38.0	39.0	35.0	39.0
10	37.38168339712802	39.0	37.0	39.0	35.0	39.0
11	37.1155048065482	39.0	37.0	39.0	33.0	39.0
12	36.64190671203335	39.0	35.0	39.0	33.0	39.0
13	36.4507705601699	39.0	35.0	39.0	33.0	39.0
14	37.57524646185381	40.0	35.0	41.0	33.0	41.0
15	37.67114368242196	40.0	35.0	41.0	33.0	41.0
16	37.76756444603329	40.0	35.0	41.0	33.0	41.0
17	37.68259668888111	40.0	35.0	41.0	33.0	41.0
18	37.605563728937966	39.0	36.0	41.0	33.0	41.0
19	37.477078862504904	39.0	36.0	41.0	33.0	41.0
20	37.26186903808749	39.0	35.0	41.0	33.0	41.0
21	37.15355887638144	39.0	35.0	41.0	32.0	41.0
22	37.04899432140889	39.0	35.0	41.0	32.0	41.0
23	36.99721919319373	38.0	35.0	41.0	32.0	41.0
24	37.003247659128	38.0	35.0	41.0	32.0	41.0
25	36.97048063366689	38.0	35.0	41.0	32.0	41.0
26	36.746691049317754	38.0	35.0	41.0	32.0	41.0
27	36.76211304087549	38.0	35.0	41.0	31.0	41.0
28	36.783832990826895	38.0	35.0	41.0	32.0	41.0
29	36.7215601371154	38.0	35.0	41.0	31.0	41.0
30	36.61049666783362	38.0	35.0	41.0	31.0	41.0
31	36.404597871348024	38.0	35.0	41.0	31.0	41.0
32	36.21892539352985	38.0	35.0	41.0	30.0	41.0
33	35.942738563757494	38.0	35.0	41.0	30.0	41.0
34	35.66090318049755	38.0	35.0	41.0	28.0	41.0
35	35.36029788012662	38.0	35.0	41.0	26.0	41.0
36	35.179041567202304	38.0	35.0	40.0	25.0	41.0
37	35.08199085967883	38.0	34.0	40.0	25.0	41.0
38	35.02558803402712	38.0	34.0	40.0	24.0	41.0
39	34.898361574690554	38.0	34.0	40.0	24.0	41.0
40	34.81015885036294	38.0	34.0	40.0	23.0	41.0
41	34.791097018343045	38.0	34.0	40.0	23.0	41.0
42	34.742530627268025	38.0	34.0	40.0	23.0	41.0
43	34.59459195901346	38.0	34.0	40.0	23.0	41.0
44	34.4709154388084	37.0	34.0	40.0	23.0	41.0
45	34.430975133291945	37.0	34.0	40.0	23.0	41.0
46	34.35363566269444	37.0	34.0	40.0	23.0	41.0
47	34.32857244090574	37.0	34.0	40.0	23.0	41.0
48	34.19948745664905	37.0	33.0	40.0	23.0	41.0
49	34.09305337706416	36.0	33.0	40.0	22.0	41.0
50	33.87145378912955	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	10.0
13	11.0
14	31.0
15	101.0
16	240.0
17	622.0
18	1445.0
19	3110.0
20	5683.0
21	9474.0
22	14771.0
23	22343.0
24	34053.0
25	53395.0
26	81073.0
27	102535.0
28	104364.0
29	100126.0
30	107757.0
31	126358.0
32	155160.0
33	202111.0
34	343937.0
35	558236.0
36	326355.0
37	419994.0
38	665709.0
39	1288399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.479893302985467	21.901677558829842	30.439786732890457	17.178642405294237
2	32.88085112233879	24.341641979056163	30.27921237973053	12.49829451887452
3	27.101337837566277	24.144493649264238	35.758095614824626	12.996072898344863
4	24.5373941940663	26.589640616786586	34.36915178623367	14.503813402913437
5	22.195961632227405	31.108568019875598	32.711730854453975	13.98373949344302
6	20.046981377732603	39.52534212744624	29.405900277213398	11.021776217607758
7	85.43422871533114	3.207679477430007	9.714949322091083	1.6431424851477714
8	86.75499560541142	2.3121353046756097	9.261571623332463	1.6712974665805025
9	81.93630543606905	5.178887783043764	10.599197657065556	2.2856091238216316
10	38.86548751376284	36.26471605457963	15.329742215866846	9.540054215790693
11	33.1502589687154	21.40358188054546	25.507926653206148	19.938232497533
12	30.302650185461154	24.987048920073487	26.963883991322934	17.74641690314242
13	19.404916650889863	32.07956585060937	27.5586711948733	20.956846303627465
14	14.944731834907312	35.91702001415153	29.47788480149257	19.660363349448588
15	18.68562139270911	23.061320111139196	40.71497999430977	17.53807850184192
16	18.280959638533194	24.444594867585916	37.62721831533368	19.647227178547215
17	17.077889455208513	20.982716733599087	34.873191528967794	27.066202282224605
18	19.00941425581265	21.911196523251128	34.94291265504013	24.13647656589609
19	22.74880193256131	21.992805778222937	27.819681199304902	27.43871108991085
20	24.313783143183205	25.401716163518884	28.38870373915499	21.895796954142916
21	19.775119753860736	24.92206612295752	29.25344877369297	26.049365349488777
22	20.35383048416626	25.804918343150206	28.0459998667345	25.795251305949034
23	17.522107794868433	31.257296550644593	27.297386198136188	23.92320945635079
24	22.20770168834699	23.583615112307914	35.53368074027929	18.67500245906581
25	17.729113541149953	24.72130058668551	32.56524456863755	24.98434130352699
26	18.725854882329735	26.681805345638885	32.8072801039894	21.78505966804198
27	17.038988620606865	25.804537584573357	31.15195334438238	26.004520450437397
28	14.815464298066274	23.111664856300656	35.698528050801656	26.374342794831414
29	15.146914639215384	19.791555832428152	40.49528229546654	24.566247232889925
30	22.51397965691537	23.932961106569035	33.655335220908725	19.897724015606872
31	24.324254004046615	28.054143869628263	28.3593430222289	19.26225910409622
32	24.655196667093257	24.482226506931394	27.298380401086852	23.564196424888493
33	23.305026753578336	29.607871548978775	26.44239281381646	20.64470888362643
34	23.244824591927284	26.58636186237481	28.319130685862543	21.849682859835365
35	25.736212573282806	24.46826535911351	29.47811748728954	20.317404580314147
36	26.624056961483095	28.154854513205446	26.275070572544557	18.9460179527669
37	20.92716828788733	28.740651583691264	32.89130082994794	17.440879298473476
38	25.01114247668647	27.686161858355696	26.903533756891996	20.399161908065842
39	25.76900011740056	27.400994837548296	27.677785169664965	19.15221987538618
40	24.188323192110683	24.351964767139687	26.19688814476441	25.26282389598522
41	19.526992081279264	22.251065859599507	32.18852626335167	26.03341579576956
42	20.32307365245838	23.29167905013427	32.284942796312144	24.10030450109521
43	19.762681640350255	24.576231568905136	28.532334335645032	27.128752455099576
44	18.853937836931678	25.224557659011655	27.86922212080412	28.052282383252546
45	17.40582835614888	28.239065618452408	25.4230809503311	28.932025075067614
46	20.011274684525652	26.904316427299968	31.302120296441704	21.782288591732673
47	20.30972594901431	23.849448058712973	33.558009098014665	22.282816894258055
48	20.545775113407885	21.82038560267208	31.694893921718155	25.93894536220189
49	24.123911532859992	22.007570749703063	30.801465074390705	23.06705264304624
50	20.52529876327499	30.62428541662921	28.094694658062934	20.75572116203287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1288.0
1	4481.5
2	7675.0
3	45189.5
4	82704.0
5	57251.5
6	31799.0
7	31562.0
8	31325.0
9	31956.0
10	32587.0
11	32932.0
12	33277.0
13	33421.5
14	33566.0
15	33583.0
16	33600.0
17	30920.0
18	28240.0
19	24745.5
20	21251.0
21	19046.5
22	16842.0
23	20340.0
24	23838.0
25	26065.5
26	28293.0
27	31839.0
28	35385.0
29	46186.5
30	56988.0
31	66991.0
32	76994.0
33	90986.5
34	104979.0
35	122658.0
36	140337.0
37	150043.5
38	159750.0
39	172321.5
40	184893.0
41	227722.5
42	270552.0
43	318125.5
44	365699.0
45	368793.5
46	371888.0
47	352798.5
48	333709.0
49	333920.5
50	334132.0
51	341426.0
52	348720.0
53	365088.5
54	381457.0
55	362656.5
56	343856.0
57	298636.0
58	253416.0
59	214494.5
60	175573.0
61	149614.0
62	123655.0
63	108176.0
64	92697.0
65	76808.0
66	60919.0
67	51055.5
68	41192.0
69	33489.0
70	25786.0
71	21260.0
72	16734.0
73	13515.0
74	10296.0
75	8134.0
76	5972.0
77	4517.5
78	3063.0
79	2248.0
80	1433.0
81	946.0
82	459.0
83	382.0
84	305.0
85	221.0
86	137.0
87	103.0
88	69.0
89	55.5
90	42.0
91	29.5
92	17.0
93	14.5
94	12.0
95	8.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4727405.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.186920016671756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58024032394275	11.289985043832923
2	8.912685068179133	2.528871003920841
3	2.933120904395796	1.2483585500967327
4	1.3439938666344466	0.7626853395536121
5	0.7917396599839832	0.5616173615109832
6	0.5425602252553309	0.46183551119548755
7	0.37196720506217074	0.369394829092937
8	0.27536231043837134	0.31252344590360903
9	0.2210010497716366	0.28217917950396326
>10	2.2477439661681533	7.315666892084328
>50	0.8341982845121634	8.656077543517211
>100	1.8817633296121663	58.85457657138578
>500	0.05907924846934352	4.991177079215732
>1k	0.003938616564622902	0.761450866733662
>5k	1.5148525248549622E-4	0.13320178578124345
>10k+	4.5445575745648863E-4	1.4703989966710063
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47389	1.0024315665782813	No Hit
GGCTTCCCTTCGACTCTTCCTCCTTTGCCTCGCTGGACTGGTATTTGTGT	10633	0.2249225526478057	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	10397	0.21993038464019904	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	6198	0.1311078699624847	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029318410417554664	0.0
2	0.0	0.0	0.0	0.11558138132865706	0.0
3	0.0	0.0	0.0	0.16647611110112207	0.0
4	0.0	0.0	0.0	0.2378471910064824	0.0
5	0.0	0.0	0.0	0.3975965672498971	0.0
6	0.0	0.0	0.0	0.5492442471080857	0.0
7	0.0	0.0	0.0	0.666137130201453	0.0
8	0.0	0.0	0.0	0.9314835517583113	0.0
9	0.0	0.0	0.0	1.0677528157625589	0.0
10	0.0	0.0	0.0	1.3068692020252126	0.0
11	0.0	0.0	0.0	1.5111673317602363	0.0
12	0.0	0.0	0.0	1.7155923810208773	0.0
13	0.0	0.0	0.0	1.7856942656700663	0.0
14	0.0	0.0	0.0	1.818227970736588	0.0
15	0.0	0.0	0.0	1.8674938999303001	0.0
16	0.0	0.0	0.0	1.9705525547314013	0.0
17	0.0	0.0	0.0	2.110037113384616	0.0
18	0.0	0.0	0.0	2.3023201946945524	0.0
19	0.0	0.0	0.0	2.387504349637909	0.0
20	0.0	0.0	0.0	2.486120821042411	0.0
21	0.0	0.0	0.0	2.6048117307486875	0.0
22	0.0	0.0	0.0	2.7357080681684773	0.0
23	0.0	0.0	0.0	2.8784502279791977	0.0
24	2.115325426951996E-5	0.0	0.0	2.9844280318694927	0.0
25	2.115325426951996E-5	0.0	0.0	3.0839329399533146	0.0
26	2.115325426951996E-5	0.0	0.0	3.1747227072780944	0.0
27	2.115325426951996E-5	0.0	0.0	3.26217026042829	0.0
28	2.115325426951996E-5	0.0	0.0	3.3586079466430316	0.0
29	2.115325426951996E-5	0.0	0.0	3.462005053512445	0.0
30	2.115325426951996E-5	0.0	0.0	3.591526429404716	0.0
31	2.115325426951996E-5	0.0	0.0	3.701417585334872	0.0
32	2.115325426951996E-5	0.0	0.0	3.8017263170809357	0.0
33	2.115325426951996E-5	0.0	0.0	3.917032706104089	0.0
34	2.115325426951996E-5	0.0	0.0	4.050636660070377	0.0
35	2.115325426951996E-5	0.0	0.0	4.209201454074699	0.0
36	2.115325426951996E-5	0.0	0.0	4.347014905640621	0.0
37	2.115325426951996E-5	0.0	0.0	4.47664204780424	0.0
38	2.115325426951996E-5	0.0	0.0	4.611980568620628	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAGT	20	7.858972E-4	44.0	32
CCACGTA	20	7.858972E-4	44.0	14
ACGCGCT	25	4.4449516E-5	44.0	34
CGTTTTT	31690	0.0	43.0142	1
CTATGCG	100	0.0	41.8	1
CGCATCG	75	0.0	41.066666	21
TCGGGTA	130	0.0	40.615387	5
TCGATAG	245	0.0	40.40816	1
CCGCTCG	285	0.0	40.14035	19
TACGGGA	2270	0.0	39.34802	4
TAGGGAC	8265	0.0	39.31518	5
ACGGGAC	3055	0.0	39.175125	5
CGGTCTA	680	0.0	39.14706	31
ATGCGAA	45	2.3535904E-8	39.11111	32
TCGACCA	45	2.3535904E-8	39.11111	24
ATAGGGA	7935	0.0	39.037174	4
GTAGGGC	1590	0.0	39.018867	4
TAGGGTA	1690	0.0	38.923077	5
AGGGACA	19105	0.0	38.89872	6
TGGGCGA	1975	0.0	38.764557	6
>>END_MODULE