FastQCFastQC Report
Thu 26 May 2016
SRR1545859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545859_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2232425
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228711.0244913042991366No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA50750.22733126532806253No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC408.3182385E-944.00000433
CGAATCG408.3182385E-944.0000042
CGTAACG207.858076E-444.00000435
CGGTCTA1600.044.00000431
CTTACGC207.858076E-444.00000411
TACGATT302.5287936E-644.044
CTACCGT551.8189894E-1244.011
TTACGGC254.4441927E-544.012
CGTCGCG254.4441927E-544.041
GCGGATA254.4441927E-544.05
CGTTTTT195450.043.2683531
CGTTAGG3000.042.5333372
TATTGCG850.041.4117661
CGGACGG1950.040.6153832
CGTAAGG3650.040.3835642
GCGTAAG2350.039.3191531
TTTACGG2700.039.111112
GAATACG452.3526809E-839.111111
ATATACG900.039.111111
AACACGT1250.038.7241
Produced by FastQC (version 0.11.5)