##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545859_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2232425
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503455211261297	31.0	31.0	33.0	30.0	34.0
2	31.89856635721245	31.0	31.0	34.0	30.0	34.0
3	31.9125623481192	31.0	31.0	34.0	30.0	34.0
4	35.69522693931487	37.0	35.0	37.0	33.0	37.0
5	35.65004737001243	37.0	35.0	37.0	33.0	37.0
6	35.706653974937566	37.0	35.0	37.0	33.0	37.0
7	36.02537688836131	37.0	35.0	37.0	35.0	37.0
8	36.02143185101403	37.0	35.0	37.0	35.0	37.0
9	37.808493454427364	39.0	38.0	39.0	35.0	39.0
10	37.31804159154283	39.0	37.0	39.0	34.0	39.0
11	37.05577298229504	39.0	37.0	39.0	33.0	39.0
12	36.492756979517786	38.0	35.0	39.0	33.0	39.0
13	36.23779074325005	38.0	35.0	39.0	32.0	39.0
14	37.30333068300167	39.0	35.0	41.0	32.0	41.0
15	37.46491550667996	39.0	35.0	41.0	33.0	41.0
16	37.58058344625239	39.0	35.0	41.0	33.0	41.0
17	37.49068031400831	39.0	35.0	41.0	33.0	41.0
18	37.33851797932741	39.0	36.0	41.0	33.0	41.0
19	37.189261005408916	38.0	35.0	41.0	32.0	41.0
20	36.978167687604284	38.0	35.0	41.0	32.0	41.0
21	36.847694771380894	38.0	35.0	41.0	32.0	41.0
22	36.74412219895405	38.0	35.0	40.0	32.0	41.0
23	36.66458358063541	38.0	35.0	40.0	32.0	41.0
24	36.70912438267803	38.0	35.0	40.0	32.0	41.0
25	36.688466129881185	38.0	35.0	40.0	32.0	41.0
26	36.44973694525012	38.0	35.0	40.0	31.0	41.0
27	36.423193164384024	38.0	35.0	40.0	31.0	41.0
28	36.41884721771168	38.0	35.0	40.0	31.0	41.0
29	36.39360426442098	38.0	35.0	40.0	31.0	41.0
30	36.25407034950782	38.0	35.0	40.0	31.0	41.0
31	36.026813442780835	38.0	35.0	40.0	30.0	41.0
32	35.73063686350045	38.0	35.0	40.0	30.0	41.0
33	35.37327345823488	38.0	34.0	40.0	27.0	41.0
34	35.02879962372756	38.0	34.0	40.0	25.0	41.0
35	34.71026843007044	38.0	34.0	40.0	23.0	41.0
36	34.52384066653975	38.0	34.0	40.0	22.0	41.0
37	34.429895293234935	38.0	34.0	40.0	22.0	41.0
38	34.36834787282888	37.0	34.0	40.0	22.0	41.0
39	34.26319271644064	37.0	34.0	40.0	21.0	41.0
40	34.14183141650895	37.0	33.0	40.0	21.0	41.0
41	34.0984512357638	37.0	34.0	40.0	20.0	41.0
42	34.06808112254611	37.0	33.0	40.0	20.0	41.0
43	33.9666398647211	37.0	33.0	40.0	19.0	41.0
44	33.842536255417315	37.0	33.0	40.0	18.0	41.0
45	33.73400181417069	37.0	33.0	40.0	18.0	41.0
46	33.67976930915932	36.0	33.0	40.0	18.0	41.0
47	33.60601050427226	36.0	33.0	40.0	18.0	41.0
48	33.48314321869716	36.0	33.0	40.0	18.0	41.0
49	33.4443446028422	36.0	33.0	40.0	18.0	41.0
50	33.27367503947501	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	0.0
12	0.0
13	8.0
14	7.0
15	33.0
16	96.0
17	296.0
18	702.0
19	1606.0
20	2906.0
21	4932.0
22	8015.0
23	12464.0
24	19545.0
25	30955.0
26	46804.0
27	57219.0
28	57232.0
29	54586.0
30	57665.0
31	64879.0
32	79235.0
33	101946.0
34	171646.0
35	276662.0
36	149933.0
37	190904.0
38	303239.0
39	538907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.94424224777988	22.161864340347382	29.663572124483466	17.230321287389273
2	34.74177184003942	24.12345319551609	28.98914857162055	12.145626392823946
3	26.905808705779588	23.785838270042667	36.73812110149277	12.570231922684972
4	23.92411839143532	25.93524978442725	36.75276885001736	13.387862974120072
5	21.747023976169412	31.664535202750372	33.79535706686675	12.793083754213466
6	19.71286829344771	39.09045096699777	31.03244229929337	10.164238440261151
7	84.35306897208193	3.1198808470609314	11.219548249101313	1.307501931755826
8	85.82653392611174	2.205762791583144	10.864597914823566	1.1031053674815503
9	80.68100832054829	5.643190700695432	11.921789085859547	1.7540118928967379
10	37.107360829591144	39.00117585137239	16.193690717493308	7.697772601543165
11	35.12046317345487	20.313649954645733	31.37449186422836	13.19139500767103
12	32.57251643392276	21.62527296549716	31.791124001926157	14.011086598653929
13	21.02435692128515	32.77382218887532	32.20578518875214	13.996035701087383
14	15.280289371423454	35.22465480363282	34.10273581419309	15.392320010750641
15	14.89469970995666	23.980693640323864	46.9097954018612	14.21481124785827
16	17.9098513947837	21.438525370393183	44.097338096464604	16.554285138358512
17	18.371188281801178	20.324445390102692	33.546927668342725	27.757438659753408
18	20.305228619102543	22.94012116868428	37.65362778144842	19.101022430764754
19	24.689698422119445	23.01752578474081	33.46791045611835	18.8248653370214
20	26.890533836523062	23.83439533242998	31.619920042106674	17.655150788940276
21	22.357973952092454	24.9720371345062	34.40917388042152	18.26081503297983
22	22.005756072432444	22.221530398557622	32.904397684132725	22.86831584487721
23	17.68435669731346	27.7119276123498	32.36480508863679	22.238910601699946
24	18.334860073686688	24.00062712073194	40.623044447181876	17.0414683583995
25	17.565562112948925	25.461684043136945	38.13503253188797	18.837721312026158
26	17.887051076743898	30.69066150038635	32.979517788951476	18.442769633918275
27	17.85255943648723	29.935787316483196	34.535404324893335	17.676248922136242
28	15.536423396082736	26.62682956874251	41.03560030012206	16.80114673505269
29	16.615160643694637	23.731233972025937	39.267522985094686	20.386082399184744
30	18.610479635374087	27.96456767864542	35.191283021826045	18.23366966415445
31	25.080170666427765	25.515974780787708	30.991052330985365	18.412802221799165
32	26.249661242818906	25.777797686372445	31.266044771940827	16.706496298867823
33	25.508583715018425	26.08226480172906	29.93511540141326	18.474036081839255
34	20.166276582639956	27.207901721222438	31.536423396082736	21.089398300054874
35	20.017290614466333	26.064257477854795	33.18010683449612	20.73834507318275
36	27.070741458279674	25.211552459769088	29.756609964500484	17.961096117450754
37	20.46774247735086	30.26309954421761	31.03656337838897	18.232594600042553
38	19.58932550925563	31.466141079767517	28.16860588821573	20.775927522761123
39	20.60131919325397	30.564520644590527	30.188337794102825	18.645822368052677
40	22.964713260243904	27.328980816824753	28.52696059218115	21.179345330750195
41	18.8777226558563	26.46749610849189	29.244789858561877	25.409991377089934
42	21.62249571654143	26.841842391121762	27.864900276605038	23.670761615731774
43	21.93023281857173	26.915842637490623	28.479881742947693	22.674042800989955
44	20.55464349306248	28.90587589728658	29.71141247746285	20.82806813218809
45	19.161002049340965	33.21876434818639	26.63802815324143	20.982205449231216
46	21.896950625440944	30.716910982451818	28.476522167598016	18.90961622450922
47	21.554005173746038	27.43809982418222	29.431134304623896	21.576760697447842
48	21.96445569280043	25.932517329809514	31.516534710012657	20.586492267377405
49	20.881507777416935	24.846254633414336	32.67424437551094	21.597993213657794
50	19.707940916268182	31.340896110731602	28.842805469388672	20.108357503611543
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	481.0
1	1474.5
2	2468.0
3	23032.0
4	43596.0
5	32444.5
6	21293.0
7	21407.5
8	21522.0
9	22105.5
10	22689.0
11	22477.5
12	22266.0
13	21009.0
14	19752.0
15	18616.0
16	17480.0
17	16062.0
18	14644.0
19	13785.0
20	12926.0
21	13517.5
22	14109.0
23	13939.5
24	13770.0
25	16915.0
26	20060.0
27	22959.0
28	25858.0
29	31113.5
30	36369.0
31	41574.0
32	46779.0
33	54811.5
34	62844.0
35	70253.0
36	77662.0
37	85661.5
38	93661.0
39	106822.0
40	119983.0
41	143348.5
42	166714.0
43	186909.5
44	207105.0
45	207855.0
46	208605.0
47	195703.0
48	182801.0
49	177760.0
50	172719.0
51	160392.5
52	148066.0
53	133498.5
54	118931.0
55	102538.0
56	86145.0
57	76644.0
58	67143.0
59	62030.5
60	56918.0
61	49177.5
62	41437.0
63	33642.0
64	25847.0
65	20806.5
66	15766.0
67	12723.0
68	9680.0
69	8237.0
70	6794.0
71	4963.5
72	3133.0
73	2708.0
74	2283.0
75	1655.0
76	1027.0
77	765.5
78	504.0
79	392.0
80	280.0
81	230.5
82	181.0
83	130.5
84	80.0
85	56.5
86	33.0
87	22.0
88	11.0
89	7.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	2.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2232425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.312843927347455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76715661359486	15.598434901339864
2	7.505064324338395	2.8988827192130167
3	2.3999741593105655	1.3905097910529562
4	1.21933144975372	0.9419503193919961
5	0.743705948504229	0.7181538455651041
6	0.5309298609914155	0.6152259325017292
7	0.39154992168421365	0.5293359769076637
8	0.3202812435732775	0.49484333359899707
9	0.25284647713361463	0.4394866095415013
>10	3.3215297176856646	15.529889692353315
>50	1.2676152211239229	17.694868884720922
>100	1.2743824327849365	41.31630088726203
>500	0.005163243727828942	0.5654402638287908
>1k	0.0	0.0
>5k	2.3469289671949732E-4	0.2300288047239369
>10k+	2.3469289671949732E-4	1.0366480379982583
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22871	1.0244913042991366	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5075	0.22733126532806253	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.479433799567734E-5	0.0	0.0	0.03695532884643381	0.0
2	4.479433799567734E-5	0.0	0.0	0.1297244028354816	0.0
3	4.479433799567734E-5	0.0	0.0	0.1835224027682901	0.0
4	4.479433799567734E-5	0.0	0.0	0.266974254454237	0.0
5	4.479433799567734E-5	0.0	0.0	0.4588284040897231	0.0
6	4.479433799567734E-5	0.0	0.0	0.6133688701748099	0.0
7	4.479433799567734E-5	0.0	0.0	0.7218159624623448	0.0
8	4.479433799567734E-5	0.0	0.0	1.0017805749353281	0.0
9	4.479433799567734E-5	0.0	0.0	1.1075400069431225	0.0
10	4.479433799567734E-5	0.0	0.0	1.3126084862873333	0.0
11	4.479433799567734E-5	0.0	0.0	1.5140486242538944	0.0
12	4.479433799567734E-5	0.0	0.0	1.7100238529849827	0.0
13	4.479433799567734E-5	0.0	0.0	1.7819635598060406	0.0
14	4.479433799567734E-5	0.0	0.0	1.809243311645408	0.0
15	4.479433799567734E-5	0.0	0.0	1.8636236379721602	0.0
16	8.958867599135469E-5	0.0	0.0	1.9809400091828393	0.0
17	8.958867599135469E-5	0.0	0.0	2.141169356193377	0.0
18	8.958867599135469E-5	0.0	0.0	2.36093037840017	0.0
19	8.958867599135469E-5	0.0	0.0	2.4669585764359385	0.0
20	8.958867599135469E-5	0.0	0.0	2.585977132490453	0.0
21	8.958867599135469E-5	0.0	0.0	2.7242572538831094	0.0
22	8.958867599135469E-5	0.0	0.0	2.8882941196232794	0.0
23	8.958867599135469E-5	0.0	0.0	3.0614242359765726	0.0
24	8.958867599135469E-5	0.0	0.0	3.192044525571968	0.0
25	8.958867599135469E-5	0.0	0.0	3.302641746083295	0.0
26	8.958867599135469E-5	0.0	0.0	3.420540443687918	0.0
27	8.958867599135469E-5	0.0	0.0	3.5398725601084022	0.0
28	8.958867599135469E-5	0.0	0.0	3.6751514608553477	0.0
29	8.958867599135469E-5	0.0	0.0	3.8170599236256537	0.0
30	8.958867599135469E-5	0.0	0.0	3.9856658118413835	0.0
31	8.958867599135469E-5	0.0	0.0	4.149075556849614	0.0
32	8.958867599135469E-5	0.0	0.0	4.294701949673561	0.0
33	8.958867599135469E-5	0.0	0.0	4.441000257567444	0.0
34	8.958867599135469E-5	0.0	0.0	4.607456017559381	0.0
35	8.958867599135469E-5	0.0	0.0	4.802714536882538	0.0
36	1.3438301398703204E-4	0.0	0.0	4.981712711513265	0.0
37	1.3438301398703204E-4	0.0	0.0	5.158023225864251	0.0
38	1.3438301398703204E-4	0.0	0.0	5.335184832637155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	40	8.3182385E-9	44.000004	33
CGAATCG	40	8.3182385E-9	44.000004	2
CGTAACG	20	7.858076E-4	44.000004	35
CGGTCTA	160	0.0	44.000004	31
CTTACGC	20	7.858076E-4	44.000004	11
TACGATT	30	2.5287936E-6	44.0	44
CTACCGT	55	1.8189894E-12	44.0	11
TTACGGC	25	4.4441927E-5	44.0	12
CGTCGCG	25	4.4441927E-5	44.0	41
GCGGATA	25	4.4441927E-5	44.0	5
CGTTTTT	19545	0.0	43.268353	1
CGTTAGG	300	0.0	42.533337	2
TATTGCG	85	0.0	41.411766	1
CGGACGG	195	0.0	40.615383	2
CGTAAGG	365	0.0	40.383564	2
GCGTAAG	235	0.0	39.319153	1
TTTACGG	270	0.0	39.11111	2
GAATACG	45	2.3526809E-8	39.11111	1
ATATACG	90	0.0	39.11111	1
AACACGT	125	0.0	38.72	41
>>END_MODULE