FastQCFastQC Report
Thu 26 May 2016
SRR1545858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545858_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1196442
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99900.8349757029592743No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18330.15320425060303802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG302.527804E-644.0000041
CTATACG207.8566105E-444.0000041
ATTACGA207.8566105E-444.00000435
TAACGCG207.8566105E-444.0000041
CTCGCGA207.8566105E-444.00000415
GCGATCG207.8566105E-444.0000049
TACCGTA302.527804E-644.00000422
TTGTCGA254.4429496E-544.032
ATCCCGC254.4429496E-544.033
ATCGATC351.4461511E-744.031
CGTTTTT69250.042.3162461
GTATGCG750.041.0666661
TATGACG603.6379788E-1240.3333361
TAATGCG603.6379788E-1240.3333361
GGCGATA4600.039.6956528
CGTTAGG1400.039.2857132
CATATCG452.3510438E-839.111111
ATAGCGG1500.038.133332
AGTACGG1500.038.133332
ACGGGAT7800.037.794875
Produced by FastQC (version 0.11.5)