##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545858_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1196442
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.509636906761884	31.0	31.0	33.0	30.0	34.0
2	31.924782814377963	31.0	31.0	34.0	30.0	34.0
3	32.01869125289818	33.0	31.0	34.0	30.0	34.0
4	35.76557910872403	37.0	35.0	37.0	35.0	37.0
5	35.69583314527574	37.0	35.0	37.0	33.0	37.0
6	35.741086488103896	37.0	35.0	37.0	33.0	37.0
7	35.9819021732771	37.0	35.0	37.0	35.0	37.0
8	35.95911627976952	37.0	35.0	37.0	35.0	37.0
9	37.71403126938038	39.0	38.0	39.0	35.0	39.0
10	37.27500371936124	39.0	37.0	39.0	34.0	39.0
11	37.04371127058395	39.0	37.0	39.0	33.0	39.0
12	36.5544205235189	39.0	35.0	39.0	33.0	39.0
13	36.34621151714835	39.0	35.0	39.0	32.0	39.0
14	37.43218392533863	40.0	35.0	41.0	32.0	41.0
15	37.53057649263399	40.0	35.0	41.0	33.0	41.0
16	37.62126538520045	40.0	35.0	41.0	33.0	41.0
17	37.55444141880676	39.0	35.0	41.0	33.0	41.0
18	37.420957305076215	39.0	36.0	41.0	32.0	41.0
19	37.33189573752844	39.0	36.0	41.0	32.0	41.0
20	37.17847334012012	39.0	35.0	41.0	32.0	41.0
21	37.049988215057645	39.0	35.0	41.0	32.0	41.0
22	36.96013346238263	39.0	35.0	41.0	32.0	41.0
23	36.86908099180737	38.0	35.0	41.0	32.0	41.0
24	36.934418885328334	39.0	35.0	41.0	32.0	41.0
25	36.901064991031745	38.0	35.0	41.0	32.0	41.0
26	36.67641306473695	38.0	35.0	41.0	31.0	41.0
27	36.65897218586442	38.0	35.0	41.0	31.0	41.0
28	36.63388112419992	38.0	35.0	41.0	31.0	41.0
29	36.59680118217181	38.0	35.0	41.0	31.0	41.0
30	36.48571263797159	38.0	35.0	41.0	31.0	41.0
31	36.34181932763978	38.0	35.0	41.0	30.0	41.0
32	36.15948203088825	38.0	35.0	41.0	30.0	41.0
33	35.92755854441753	38.0	35.0	41.0	29.0	41.0
34	35.679829862208116	38.0	35.0	41.0	28.0	41.0
35	35.44946182096583	38.0	35.0	41.0	27.0	41.0
36	35.303591816402296	38.0	34.0	40.0	25.0	41.0
37	35.242986287676295	38.0	34.0	40.0	25.0	41.0
38	35.163475538304404	38.0	34.0	40.0	25.0	41.0
39	35.04430553257074	38.0	34.0	40.0	24.0	41.0
40	34.936717366993136	38.0	34.0	40.0	24.0	41.0
41	34.88145100222159	38.0	34.0	40.0	24.0	41.0
42	34.86542097318549	38.0	34.0	40.0	24.0	41.0
43	34.7855750633963	38.0	34.0	40.0	23.0	41.0
44	34.67046459418844	37.0	34.0	40.0	23.0	41.0
45	34.56995742376145	37.0	34.0	40.0	23.0	41.0
46	34.52017231090182	37.0	34.0	40.0	23.0	41.0
47	34.46884930485556	37.0	34.0	40.0	23.0	41.0
48	34.354909807579475	37.0	34.0	40.0	23.0	41.0
49	34.27320421717058	37.0	34.0	40.0	23.0	41.0
50	34.15198145835736	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	1.0
12	1.0
13	3.0
14	12.0
15	37.0
16	95.0
17	191.0
18	481.0
19	931.0
20	1638.0
21	2587.0
22	4063.0
23	5766.0
24	8621.0
25	13205.0
26	19272.0
27	24227.0
28	25961.0
29	26408.0
30	28627.0
31	33128.0
32	40550.0
33	52220.0
34	89020.0
35	133163.0
36	82293.0
37	108054.0
38	173330.0
39	322553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.764897922339735	22.767505654264895	28.72182688337588	15.745769540019491
2	33.137669857795025	24.60955065101359	29.309820283808158	12.942959207383225
3	28.327658173150056	24.472728306094236	33.96403670215523	13.235576818600483
4	26.115515837792387	26.950157216145872	32.406501944933396	14.527825001128344
5	23.29080724347691	30.90889487329933	31.652265634272286	14.148032248951475
6	21.293635629641887	39.735983858808034	28.61007888389074	10.360301627659343
7	85.34947786854691	4.104503185277681	8.917440210223312	1.6285787359520978
8	86.44372230329594	3.0590701429739178	8.807865320675804	1.6893422330543393
9	80.64619931430023	6.182414191410866	10.299872455162891	2.8715140391260086
10	38.067620494766985	36.53833616673437	15.150337417108393	10.243705921390255
11	36.49788288943384	22.079967102458788	26.439977867711097	14.982172140396274
12	33.543456348072034	21.150293954909642	28.90537109195431	16.400878605064015
13	23.47953348344508	29.36866141442711	29.74444227133451	17.407362830793303
14	18.10050131974638	32.404579578450104	32.00940789440692	17.4855112073966
15	18.33962699403732	23.912065942185244	41.16146039674301	16.586846667034425
16	21.596951628244412	21.82220283139509	38.62184711001453	17.958998430345975
17	20.890941641968436	21.4954841103873	31.178109762111326	26.43546448553294
18	22.68559612584647	23.670516414502334	33.96587548748707	19.678011972164132
19	26.36642645443741	24.144839448966184	29.36164059770553	20.127093498890876
20	28.477352015392306	23.66809256111036	29.12953574013617	18.725019683361165
21	24.610720787133854	24.621502755670562	31.260353615135543	19.50742284206004
22	23.674695472074703	23.58116816360509	30.172294185593618	22.57184217872659
23	20.93707843756739	25.996496278131325	30.907808318330517	22.158616965970772
24	21.367604948672813	23.148886448319267	36.45559082680146	19.02791777620645
25	20.46417628267814	25.382425558447462	33.5620949448448	20.591303214029598
26	19.958510316421524	29.10370916433893	29.91971194591965	21.018068573319894
27	19.507339260908594	28.796381270466938	31.76702255520953	19.929256913414942
28	18.095402869508092	27.255729905837473	35.55349945922995	19.095367765424484
29	19.81717458932401	24.34301035904791	34.46243110823592	21.377383943392157
30	21.001268761878972	26.551642286044792	32.37666347386668	20.070425478209557
31	25.521671756758792	25.436920469191154	29.555130963306205	19.486276810743856
32	26.469732757626364	25.60282905481419	29.546772848161467	18.380665339397982
33	25.492752678358	27.024795184388378	28.656550004095475	18.82590213315815
34	22.020373741476813	26.40186486265109	30.863593889214858	20.714167506657237
35	22.000481427432337	26.557910872403344	30.998326705348024	20.443280994816295
36	25.199967904837845	27.523189590469073	27.9745278082849	19.302314696408185
37	21.646515251052705	31.055496212938028	28.598962590748233	18.699025945261035
38	21.696329617315342	30.053608950538347	26.689885510538748	21.560175921607566
39	21.879288757833642	29.16104583423183	27.904570384523446	21.05509502341108
40	23.296741505229672	27.368062973382745	27.56807266879631	21.767122852591267
41	19.468557606637013	26.860976127551524	28.60623415092416	25.064232114887307
42	21.976577218118386	26.870755122270868	27.219790010715105	23.93287764889564
43	22.548105131715538	26.910790493814158	27.811210238356725	22.729894136113575
44	21.243988425682147	28.141606530028202	28.987949269584316	21.626455774705335
45	19.830046086646906	31.387564127638445	26.416742307608725	22.365647478105917
46	21.471161995316113	30.00346025966992	28.08619222661859	20.439185518395377
47	21.415413367300715	27.480479622079468	29.125941750623934	21.978165259995887
48	22.162628861240243	25.352169181623513	31.285427960569756	21.19977399656649
49	21.441323524249402	26.087599733208965	30.80099160678077	21.670085135760864
50	20.19571362422917	29.867473726265043	29.11198369833222	20.82482895117356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	288.0
1	1123.5
2	1959.0
3	11042.0
4	20125.0
5	14414.0
6	8703.0
7	8363.5
8	8024.0
9	7949.5
10	7875.0
11	7724.0
12	7573.0
13	7077.0
14	6581.0
15	5983.5
16	5386.0
17	5148.0
18	4910.0
19	4727.5
20	4545.0
21	4662.0
22	4779.0
23	5514.0
24	6249.0
25	7614.0
26	8979.0
27	11723.0
28	14467.0
29	17512.5
30	20558.0
31	24483.0
32	28408.0
33	32267.5
34	36127.0
35	39331.5
36	42536.0
37	45180.0
38	47824.0
39	52722.0
40	57620.0
41	68772.5
42	79925.0
43	85190.5
44	90456.0
45	93157.0
46	95858.0
47	91009.5
48	86161.0
49	84572.5
50	82984.0
51	80448.5
52	77913.0
53	72565.0
54	67217.0
55	62403.5
56	57590.0
57	54696.5
58	51803.0
59	49689.5
60	47576.0
61	43832.5
62	40089.0
63	34003.0
64	27917.0
65	22734.0
66	17551.0
67	14391.5
68	11232.0
69	9357.5
70	7483.0
71	6080.0
72	4677.0
73	3792.5
74	2908.0
75	2259.5
76	1611.0
77	1256.5
78	902.0
79	744.0
80	586.0
81	427.5
82	269.0
83	198.5
84	128.0
85	86.5
86	45.0
87	32.5
88	20.0
89	14.5
90	9.0
91	9.5
92	10.0
93	8.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1196442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.654132346627378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00780473193707	15.135215859247294
2	5.7456558829562745	2.2585176228360164
3	2.1676153229914874	1.2780779530395645
4	1.3670872556277818	1.0747565540598416
5	0.9272287686112577	0.9111938466942998
6	0.758256511252941	0.8941724294914355
7	0.6432002594747283	0.8849080117570965
8	0.5640439123583127	0.8868634962239818
9	0.5130892357927647	0.9075891371310808
>10	7.851149747369418	37.128423204676665
>50	2.0668504933693206	28.196250311128374
>100	0.38629908367055793	9.328653660674025
>500	8.593972940390611E-4	0.11688370043520988
>1k	4.2969864701953057E-4	0.15480334024403158
>5k	4.2969864701953057E-4	0.8436908723610889
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9990	0.8349757029592743	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1833	0.15320425060303802	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01897292137855408	0.0
2	0.0	0.0	0.0	0.06828580073250522	0.0
3	0.0	0.0	0.0	0.10263765397737626	0.0
4	0.0	0.0	0.0	0.14192079515764242	0.0
5	0.0	0.0	0.0	0.2448091925893608	0.0
6	0.0	0.0	0.0	0.3426827209342367	0.0
7	0.0	0.0	0.0	0.40612081488279417	0.0
8	0.0	0.0	0.0	0.567098112570438	0.0
9	0.0	0.0	0.0	0.6383928347550487	0.0
10	0.0	0.0	0.0	0.7659376718637426	0.0
11	0.0	0.0	0.0	0.901589880662832	0.0
12	0.0	0.0	0.0	1.0274630947425785	0.0
13	0.0	0.0	0.0	1.0767759740965295	0.0
14	0.0	0.0	0.0	1.0952474085663995	0.0
15	0.0	0.0	0.0	1.1229963508469278	0.0
16	0.0	0.0	0.0	1.1891926227932486	0.0
17	0.0	0.0	0.0	1.2732752611493077	0.0
18	0.0	0.0	0.0	1.3964738783827382	0.0
19	0.0	0.0	0.0	1.4550642655473478	0.0
20	0.0	0.0	0.0	1.5209262128878793	0.0
21	0.0	0.0	0.0	1.6036715528207803	0.0
22	0.0	0.0	0.0	1.6895093953572342	0.0
23	0.0	0.0	0.0	1.7806128504348728	0.0
24	0.0	0.0	0.0	1.8562537924947469	0.0
25	0.0	0.0	0.0	1.9196918864433044	0.0
26	0.0	0.0	0.0	1.984968765723704	0.0
27	0.0	0.0	0.0	2.049827739246867	0.0
28	0.0	0.0	0.0	2.121206042582925	0.0
29	0.0	0.0	0.0	2.1938380631906935	0.0
30	0.0	0.0	0.0	2.2844400313596482	0.0
31	0.0	0.0	0.0	2.370445036198997	0.0
32	0.0	0.0	0.0	2.4556142295238716	0.0
33	0.0	0.0	0.0	2.539278962122694	0.0
34	0.0	0.0	0.0	2.6313853910177007	0.0
35	0.0	0.0	0.0	2.736697641841393	0.0
36	0.0	0.0	0.0	2.830810018371137	0.0
37	0.0	0.0	0.0	2.934283483862987	0.0
38	0.0	0.0	0.0	3.046031483348127	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.527804E-6	44.000004	1
CTATACG	20	7.8566105E-4	44.000004	1
ATTACGA	20	7.8566105E-4	44.000004	35
TAACGCG	20	7.8566105E-4	44.000004	1
CTCGCGA	20	7.8566105E-4	44.000004	15
GCGATCG	20	7.8566105E-4	44.000004	9
TACCGTA	30	2.527804E-6	44.000004	22
TTGTCGA	25	4.4429496E-5	44.0	32
ATCCCGC	25	4.4429496E-5	44.0	33
ATCGATC	35	1.4461511E-7	44.0	31
CGTTTTT	6925	0.0	42.316246	1
GTATGCG	75	0.0	41.066666	1
TATGACG	60	3.6379788E-12	40.333336	1
TAATGCG	60	3.6379788E-12	40.333336	1
GGCGATA	460	0.0	39.695652	8
CGTTAGG	140	0.0	39.285713	2
CATATCG	45	2.3510438E-8	39.11111	1
ATAGCGG	150	0.0	38.13333	2
AGTACGG	150	0.0	38.13333	2
ACGGGAT	780	0.0	37.79487	5
>>END_MODULE