FastQCFastQC Report
Thu 26 May 2016
SRR1545857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545857_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences139427
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.6003141428848071No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA2310.1656780967818285No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA1810.1298170368723418No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAG207.8326685E-444.0000041
CGTGGGA408.221832E-944.0000044
TTAGCGG207.8326685E-444.0000042
CACATAG207.8326685E-444.00000434
TTATGAG408.221832E-944.0000041
AGGGTCA408.221832E-944.0000046
TATGCTG207.8326685E-444.0000041
TGCGGGT207.8326685E-444.0000044
CCGCTAT302.5116806E-644.00000425
ACGGGAT600.044.0000045
TAGCATA207.8326685E-444.00000430
ACCTAGG207.8326685E-444.0000042
TATTCCT207.8326685E-444.00000432
TGTTATG207.8326685E-444.0000041
TAGGGCG408.221832E-944.0000045
CATACGA600.044.00000418
GCGATGA207.8326685E-444.0000049
AAGGGCG302.5116806E-644.0000045
ATACGAA600.044.00000419
ACATACG600.044.00000417
Produced by FastQC (version 0.11.5)