##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545857_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 139427 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.413191132277106 31.0 31.0 33.0 30.0 34.0 2 31.81726638312522 31.0 31.0 34.0 30.0 34.0 3 31.962596914514407 31.0 31.0 34.0 30.0 34.0 4 35.71134715657656 37.0 35.0 37.0 33.0 37.0 5 35.626191483715495 37.0 35.0 37.0 33.0 37.0 6 35.65758425556026 37.0 35.0 37.0 33.0 37.0 7 35.89986874852073 37.0 35.0 37.0 35.0 37.0 8 35.864007688611245 37.0 35.0 37.0 35.0 37.0 9 37.614902422055984 39.0 37.0 39.0 35.0 39.0 10 37.157688252633996 39.0 37.0 39.0 33.0 39.0 11 36.9253157566325 39.0 37.0 39.0 33.0 39.0 12 36.438695518084735 38.0 35.0 39.0 32.0 39.0 13 36.215001398581336 38.0 35.0 39.0 32.0 39.0 14 37.30463970393109 39.0 35.0 41.0 32.0 41.0 15 37.418735252139115 39.0 35.0 41.0 32.0 41.0 16 37.50911946753498 39.0 35.0 41.0 32.0 41.0 17 37.41629670006527 39.0 35.0 41.0 32.0 41.0 18 37.35128777066135 39.0 35.0 41.0 32.0 41.0 19 37.27757177591141 39.0 35.0 41.0 32.0 41.0 20 37.14049646051339 39.0 35.0 41.0 32.0 41.0 21 37.01736392520817 39.0 35.0 41.0 32.0 41.0 22 36.90273763331349 39.0 35.0 41.0 31.0 41.0 23 36.8082007071801 38.0 35.0 41.0 31.0 41.0 24 36.87982958824331 38.0 35.0 41.0 32.0 41.0 25 36.85948202285067 38.0 35.0 41.0 31.0 41.0 26 36.593629641317676 38.0 35.0 41.0 31.0 41.0 27 36.58471458182418 38.0 35.0 41.0 31.0 41.0 28 36.56336290675407 38.0 35.0 41.0 31.0 41.0 29 36.53773659334275 38.0 35.0 41.0 31.0 41.0 30 36.42432957748499 38.0 35.0 41.0 30.0 41.0 31 36.30877807024464 38.0 35.0 41.0 30.0 41.0 32 36.18830642558471 38.0 35.0 41.0 30.0 41.0 33 36.01379933585317 38.0 35.0 41.0 30.0 41.0 34 35.808975306074146 38.0 35.0 41.0 29.0 41.0 35 35.62685849942981 38.0 35.0 40.0 28.0 41.0 36 35.45833303449117 38.0 35.0 40.0 27.0 41.0 37 35.381855738128195 38.0 34.0 40.0 27.0 41.0 38 35.295538166926065 38.0 34.0 40.0 26.0 41.0 39 35.16947219692025 38.0 34.0 40.0 26.0 41.0 40 35.02680255617635 38.0 34.0 40.0 25.0 41.0 41 34.99395382529926 38.0 34.0 40.0 25.0 41.0 42 34.92097656838345 38.0 34.0 40.0 24.0 41.0 43 34.79643110731781 37.0 34.0 40.0 24.0 41.0 44 34.66670013698925 37.0 34.0 40.0 24.0 41.0 45 34.533734499056855 37.0 34.0 40.0 23.0 41.0 46 34.43037575218573 37.0 34.0 40.0 23.0 41.0 47 34.37321322269001 37.0 33.0 40.0 23.0 41.0 48 34.22119101752171 36.0 33.0 40.0 23.0 41.0 49 34.19874916623036 36.0 33.0 40.0 23.0 41.0 50 34.033365130139785 36.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 0.0 14 2.0 15 7.0 16 4.0 17 33.0 18 48.0 19 111.0 20 215.0 21 330.0 22 481.0 23 662.0 24 943.0 25 1405.0 26 2077.0 27 2605.0 28 2859.0 29 3197.0 30 3518.0 31 4148.0 32 5092.0 33 6368.0 34 10639.0 35 15640.0 36 10138.0 37 12972.0 38 20166.0 39 35765.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.947513752716475 23.02136601949407 28.59991249901382 14.431207728775632 2 31.02698903368788 25.680822222381604 29.81345076635085 13.478737977579666 3 29.214571065862422 25.42549147582606 32.42772203375243 12.932215424559088 4 26.603168683253603 27.12100238834659 31.747079116670374 14.528749811729435 5 23.884183120916322 32.19605958673715 29.41539300135555 14.504364290990985 6 21.533132033250375 41.02648697884915 26.68995244823456 10.750428539665918 7 87.09360453857575 3.911724414926807 7.112682622447589 1.8819884240498612 8 87.60139714689407 3.457723396472706 6.906122917368945 2.034756539264275 9 81.57889074569488 6.6680054795699535 8.795283553400704 2.9578202213344618 10 39.437124803660694 36.60696995560401 13.192566719501961 10.763338521233333 11 37.721531697590855 21.932624240642056 24.491669475783027 15.854174585984063 12 35.31812346245706 21.420528305134585 27.083706886040726 16.177641346367633 13 23.480387586335503 30.610283517539642 27.921421245526336 17.98790765059852 14 17.37109742015535 33.3027318955439 30.54429916730619 18.781871516994556 15 17.239845940886628 24.09863225917505 40.977715937372245 17.683805862566075 16 20.230658337337818 21.54604201481779 38.5778938082294 19.645405839614995 17 20.03127084424107 21.798503876580575 29.656379323947295 28.513845955231055 18 21.78415945261678 23.497600895092056 33.02445007064629 21.693789581644875 19 26.48052385836316 24.521792766106994 28.006053346912722 20.991630028617124 20 28.450730489790356 22.963988323638894 28.05984493677695 20.5254362497938 21 23.653237895099227 25.944759623315427 29.01518357276568 21.38681890881967 22 23.253745687707546 23.446678190020585 28.43853772942113 24.86103839285074 23 21.933341461840246 27.14467068788685 27.661787171781647 23.260200678491252 24 20.645929411089675 23.8856175633127 35.01617333801918 20.452279687578446 25 20.65955661385528 25.497213595645036 31.59000767426682 22.25322211623287 26 19.734341268190523 30.445322641956007 28.17603477088369 21.644301318969784 27 20.513960710622765 29.132090628070607 29.068257941431717 21.285690719874918 28 18.438322563061675 27.165470102634355 34.420879743521695 19.975327590782275 29 19.595200355741717 23.69770560938699 33.91523879879794 22.791855236073356 30 20.430045830434565 27.047128604933047 30.787437153492508 21.73538841113988 31 25.462786978131923 25.848651982758003 27.135346812310384 21.55321422679969 32 26.15920876157416 25.203152904387245 28.0985748814792 20.539063452559404 33 25.119955245397236 27.085858549635294 26.651222503532313 21.14296370143516 34 20.291622139183946 26.989033687879676 28.93055147137929 23.78879270155709 35 21.802089982571523 25.470676411312017 29.73957698293731 22.987656623179152 36 25.756130448191527 26.3657684666528 27.245798876831607 20.63230220832407 37 21.62852245260961 29.96550166036707 27.82531360496891 20.58066228205441 38 21.906804277507224 28.871739333127728 26.363616803058232 22.857839586306813 39 22.364391401952275 28.057693273182384 26.560135411362218 23.017779913503123 40 23.012759365115794 26.25675084452796 27.544880116476723 23.18560967387952 41 19.95739706082753 25.5832801394278 28.587002517446408 25.87232028229826 42 22.258959885818385 26.574479835326017 26.09465885373708 25.07190142511852 43 22.608963830534975 26.574479835326017 26.93022154962812 23.88633478451089 44 21.127902056273175 28.384028918358712 27.727771522015104 22.76029750335301 45 19.510568254355327 31.455887310205338 25.605513996571684 23.42803043886765 46 21.81141385814799 30.26673456360676 26.537901554218337 21.38395002402691 47 22.459064600113322 26.37078901504013 27.986688374561595 23.18345801028495 48 22.74882196418197 24.53255108407984 30.073084840095532 22.64554211164265 49 20.805869738285985 25.240448406693105 30.598807978368608 23.3548738766523 50 19.718562401830347 29.03670020871137 28.812209973677984 22.4325274157803 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 45.0 1 107.5 2 170.0 3 900.0 4 1630.0 5 1167.5 6 705.0 7 695.5 8 686.0 9 675.0 10 664.0 11 632.5 12 601.0 13 577.5 14 554.0 15 520.5 16 487.0 17 448.5 18 410.0 19 437.5 20 465.0 21 493.5 22 522.0 23 609.0 24 696.0 25 812.5 26 929.0 27 1422.0 28 1915.0 29 2145.5 30 2376.0 31 2765.0 32 3154.0 33 3331.5 34 3509.0 35 3896.0 36 4283.0 37 4633.0 38 4983.0 39 5654.0 40 6325.0 41 7543.0 42 8761.0 43 9869.5 44 10978.0 45 11171.0 46 11364.0 47 11034.5 48 10705.0 49 10683.0 50 10661.0 51 10041.0 52 9421.0 53 8658.5 54 7896.0 55 7515.5 56 7135.0 57 6656.0 58 6177.0 59 5914.0 60 5651.0 61 5339.5 62 5028.0 63 4314.0 64 3600.0 65 3030.0 66 2460.0 67 2050.0 68 1640.0 69 1399.5 70 1159.0 71 954.5 72 750.0 73 592.0 74 434.0 75 343.5 76 253.0 77 193.5 78 134.0 79 92.0 80 50.0 81 38.5 82 27.0 83 23.0 84 19.0 85 12.0 86 5.0 87 4.5 88 4.0 89 4.0 90 4.0 91 2.0 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 139427.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.8585209464451 #Duplication Level Percentage of deduplicated Percentage of total 1 64.35540399734772 24.364004102505255 2 9.18063843895046 6.951307852854899 3 5.717533390167661 6.4937207284098495 4 4.486122951596097 6.793519189253158 5 3.1846168419058443 6.028244170784712 6 2.6446907265321586 6.007444756037209 7 2.135076252723312 5.658158032518809 8 1.689874017239746 5.11809047028194 9 1.329923273657289 4.531403530162738 >10 5.257175333901676 26.738723489711465 >50 0.011366865586814436 0.331356193563657 >100 0.005683432793407218 0.3837133410315075 >500 0.0018944775978024058 0.6003141428848071 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 837 0.6003141428848071 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 231 0.1656780967818285 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 181 0.1298170368723418 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.048771041476901894 0.0 2 0.0 0.0 0.0 0.13340314286329047 0.0 3 0.0 0.0 0.0 0.19651860830398704 0.0 4 0.0 0.0 0.0 0.3091223364197752 0.0 5 0.0 0.0 0.0 0.5250059170748851 0.0 6 0.0 0.0 0.0 0.7480617097118922 0.0 7 0.0 0.0 0.0 0.8929403917462184 0.0 8 0.0 0.0 0.0 1.360568612965925 0.0 9 0.0 0.0 0.0 1.5563700000717222 0.0 10 0.0 0.0 0.0 1.907091165986502 0.0 11 0.0 0.0 0.0 2.133015843416268 0.0 12 0.0 0.0 0.0 2.3582232996478445 0.0 13 0.0 0.0 0.0 2.442855401034233 0.0 14 0.0 0.0 0.0 2.466523700574494 0.0 15 0.0 0.0 0.0 2.5088397512676885 0.0 16 0.0 0.0 0.0 2.5977751798432154 0.0 17 0.0 0.0 0.0 2.696034483995209 0.0 18 0.0 0.0 0.0 2.8968564194883344 0.0 19 0.0 0.0 0.0 2.979336857280154 0.0 20 0.0 0.0 0.0 3.0854855946122344 0.0 21 0.0 0.0 0.0 3.2031098711153505 0.0 22 0.0 0.0 0.0 3.330775244393123 0.0 23 0.0 0.0 0.0 3.4620267236618445 0.0 24 0.0 0.0 0.0 3.5753476729758225 0.0 25 0.0 0.0 0.0 3.665000322749539 0.0 26 0.0 0.0 0.0 3.7474807605413587 0.0 27 0.0 0.0 0.0 3.8278095347386087 0.0 28 0.0 0.0 0.0 3.93252382967431 0.0 29 0.0 0.0 0.0 4.032217576222683 0.0 30 0.0 0.0 0.0 4.157014064707696 0.0 31 0.0 0.0 0.0 4.276790004805382 0.0 32 0.0 0.0 0.0 4.367877096975478 0.0 33 0.0 0.0 0.0 4.4725913919111795 0.0 34 0.0 0.0 0.0 4.567264590072224 0.0 35 0.0 0.0 0.0 4.6913438573590485 0.0 36 0.0 0.0 0.0 4.8118370186549235 0.0 37 7.172211981897337E-4 0.0 0.0 4.946674603914594 0.0 38 7.172211981897337E-4 0.0 0.0 5.061429995624951 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTGTAG 20 7.8326685E-4 44.000004 1 CGTGGGA 40 8.221832E-9 44.000004 4 TTAGCGG 20 7.8326685E-4 44.000004 2 CACATAG 20 7.8326685E-4 44.000004 34 TTATGAG 40 8.221832E-9 44.000004 1 AGGGTCA 40 8.221832E-9 44.000004 6 TATGCTG 20 7.8326685E-4 44.000004 1 TGCGGGT 20 7.8326685E-4 44.000004 4 CCGCTAT 30 2.5116806E-6 44.000004 25 ACGGGAT 60 0.0 44.000004 5 TAGCATA 20 7.8326685E-4 44.000004 30 ACCTAGG 20 7.8326685E-4 44.000004 2 TATTCCT 20 7.8326685E-4 44.000004 32 TGTTATG 20 7.8326685E-4 44.000004 1 TAGGGCG 40 8.221832E-9 44.000004 5 CATACGA 60 0.0 44.000004 18 GCGATGA 20 7.8326685E-4 44.000004 9 AAGGGCG 30 2.5116806E-6 44.000004 5 ATACGAA 60 0.0 44.000004 19 ACATACG 60 0.0 44.000004 17 >>END_MODULE