FastQCFastQC Report
Thu 26 May 2016
SRR1545856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545856_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4883449
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT522181.069285253106974No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA97980.20063688593860612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT343050.043.0444531
CGATACG603.6379788E-1240.33333210
CGACGGT8350.040.31137528
TATTACG1100.040.01
CGGTCTA8250.040.031
GACCGAT19150.038.71549
GTCGAAC404.1282146E-738.540
TATCGCG2000.038.51
CTAGACG1900.038.2105261
CGTATGG3800.038.2105262
CTACGGG8000.037.953
CGCGATC357.295068E-637.71428720
CGACTAT357.295068E-637.71428721
TCGACTA357.295068E-637.71428727
ACGGGAG28100.037.501785
TACGGGA26650.037.4784244
GCGGCTA13800.037.30434824
TTAGCGG12100.037.2727282
TAGGGAC51500.037.2504845
CAATACG651.0913936E-1137.230771
Produced by FastQC (version 0.11.5)