##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545856_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4883449
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46028718637176	31.0	31.0	33.0	30.0	34.0
2	31.864398706733702	31.0	31.0	34.0	30.0	34.0
3	31.936790370903843	31.0	31.0	34.0	30.0	34.0
4	35.696134023310165	37.0	35.0	37.0	33.0	37.0
5	35.624126104316844	37.0	35.0	37.0	33.0	37.0
6	35.6707431571416	37.0	35.0	37.0	33.0	37.0
7	35.96789236459723	37.0	35.0	37.0	35.0	37.0
8	35.954211459974296	37.0	35.0	37.0	35.0	37.0
9	37.71653804513982	39.0	38.0	39.0	35.0	39.0
10	37.21635978997631	39.0	37.0	39.0	33.0	39.0
11	36.980761138285665	39.0	37.0	39.0	33.0	39.0
12	36.40965985310792	38.0	35.0	39.0	32.0	39.0
13	36.18840946224687	38.0	35.0	39.0	32.0	39.0
14	37.24807036993731	39.0	35.0	41.0	32.0	41.0
15	37.36001440784986	39.0	35.0	41.0	32.0	41.0
16	37.43925594390358	39.0	35.0	41.0	33.0	41.0
17	37.32692304148154	39.0	35.0	41.0	32.0	41.0
18	37.23283339295649	39.0	35.0	41.0	32.0	41.0
19	37.119871222162864	38.0	35.0	41.0	32.0	41.0
20	36.94342441172213	38.0	35.0	41.0	32.0	41.0
21	36.83831386382862	38.0	35.0	41.0	32.0	41.0
22	36.73800217837844	38.0	35.0	41.0	31.0	41.0
23	36.655949514369865	38.0	35.0	40.0	31.0	41.0
24	36.712385242479236	38.0	35.0	41.0	32.0	41.0
25	36.6884028071144	38.0	35.0	41.0	31.0	41.0
26	36.44961460639806	38.0	35.0	41.0	31.0	41.0
27	36.4436653275175	38.0	35.0	41.0	31.0	41.0
28	36.41077750581607	38.0	35.0	41.0	31.0	41.0
29	36.3632141955409	38.0	35.0	40.0	31.0	41.0
30	36.21766296730036	38.0	35.0	40.0	30.0	41.0
31	36.05955340170441	38.0	35.0	40.0	30.0	41.0
32	35.83989758058291	38.0	35.0	40.0	30.0	41.0
33	35.55770358203802	38.0	35.0	40.0	28.0	41.0
34	35.26036393540713	38.0	34.0	40.0	26.0	41.0
35	35.047360789474816	38.0	34.0	40.0	25.0	41.0
36	34.89910163902602	38.0	34.0	40.0	24.0	41.0
37	34.82156545507079	38.0	34.0	40.0	24.0	41.0
38	34.761344901933036	38.0	34.0	40.0	24.0	41.0
39	34.648759104477186	37.0	34.0	40.0	23.0	41.0
40	34.55143342338581	37.0	34.0	40.0	23.0	41.0
41	34.51058790621137	37.0	34.0	40.0	23.0	41.0
42	34.478318295122975	37.0	34.0	40.0	23.0	41.0
43	34.368110530078226	37.0	33.0	40.0	23.0	41.0
44	34.25135861969686	37.0	33.0	40.0	23.0	41.0
45	34.160407326870825	37.0	33.0	40.0	23.0	41.0
46	34.09903615252253	37.0	33.0	40.0	22.0	41.0
47	34.04756228640864	36.0	33.0	40.0	22.0	41.0
48	33.941691210453925	36.0	33.0	40.0	22.0	41.0
49	33.88565806666559	36.0	33.0	40.0	22.0	41.0
50	33.75398760179537	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	4.0
12	16.0
13	23.0
14	37.0
15	95.0
16	350.0
17	869.0
18	1891.0
19	3954.0
20	7262.0
21	11557.0
22	17837.0
23	26674.0
24	39021.0
25	59251.0
26	87544.0
27	108963.0
28	116926.0
29	119558.0
30	129858.0
31	147168.0
32	176497.0
33	223661.0
34	381301.0
35	587384.0
36	326898.0
37	415925.0
38	657347.0
39	1235571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.503615579890358	22.838981220035265	28.563582828447682	17.09382037162669
2	32.25345447449129	24.61559442926505	30.190056249179626	12.940894847064033
3	28.141770293904983	23.92239583130693	34.739504804903255	13.196329069884829
4	24.79446391269777	27.288418492749695	33.78053093213423	14.136586662418301
5	22.50679796185032	31.288808381125715	32.24831466449224	13.956078992531712
6	20.75213645110249	39.511603377039464	28.94485024825692	10.791409923601126
7	84.54772436448093	3.5430287077841913	10.212659126776998	1.6965878009578887
8	85.50168129123495	2.7574568711580687	9.931955877905144	1.8089059597018418
9	79.72496487625857	6.318485152604235	11.315527202188454	2.641022768948749
10	37.689940040328054	36.80355830479647	16.097803007669377	9.408698647206105
11	36.43394248614043	21.238595918581314	27.938839946930948	14.388621648347305
12	34.4542555886219	20.406663405310468	29.42135773302844	15.7177232730392
13	22.679380904766283	29.526590735359378	30.026380945106624	17.76764741476772
14	16.18384875115927	32.54292202089138	33.09542087979213	18.177808348157214
15	17.352121420741774	23.330949089465253	43.2171197037176	16.099809786075376
16	20.64848020323341	21.508343795542864	39.7778905851172	18.065285416106526
17	19.007345013739265	20.985045610182475	33.08389214262297	26.923717233455292
18	21.977336099957224	24.00797059619134	34.27647140371487	19.738221900136562
19	24.69387926442971	25.71934302989547	29.682157016485682	19.904620689189137
20	27.313216540195263	24.213829201451677	30.026053307815847	18.446900950537213
21	22.660910352498817	26.515993102415937	31.21287843898851	19.610218106096735
22	22.35819397315299	23.383514397304037	32.09672098551659	22.161570644026384
23	20.572734557072266	26.435128123586427	31.146327114299748	21.84581020504156
24	21.241790382166375	23.12631912404532	37.0075739502962	18.62431654349211
25	19.196842231791507	24.912495246699617	34.044729452483274	21.845933069025598
26	18.050562215352308	29.06685418440942	31.473964405075183	21.40861919516309
27	18.00559399719338	28.160568483463223	32.61661993398518	21.217217585358217
28	16.06508023325318	27.864200076626172	36.47473332884197	19.595986361278676
29	19.263209260504205	24.057873851042572	35.559130442439354	21.119786446013872
30	19.731935359619808	26.221570041992866	33.90013902059794	20.146355577789386
31	24.898345411204254	25.728291623399773	29.25063822720377	20.122724738192208
32	26.45265671864291	25.370716475179734	29.734927097631203	18.441699708546153
33	24.796286395127705	26.77034202671104	29.061530078434323	19.371841499726937
34	19.409212628206006	27.077604373466375	32.461934178077826	21.05124882024979
35	19.698393491976674	27.22874755116722	31.241812907230116	21.83104604962599
36	23.614580596623412	28.832716385489025	28.53172010191977	19.020982915967792
37	21.133362916250377	30.628762581527937	28.985538704305092	19.252335797916594
38	20.4955350204333	30.984494769987357	26.529897209943222	21.99007299963612
39	21.752802169122685	29.144668040968586	28.18364643513222	20.91888335477651
40	22.40023393302561	26.526907519664896	28.18045197154716	22.892406575762337
41	18.08977630359199	26.22109906338737	29.40296909008367	26.286155542936967
42	21.055979083635357	26.523794965402526	26.843487051876657	25.57673889908546
43	21.119458808723095	26.419503920282573	28.25075064774916	24.21028662324517
44	20.129461779983778	28.3196363881347	29.146900070011995	22.404001761869534
45	19.566150890487442	31.332261276814812	26.638160857213826	22.463426975483923
46	20.436150761480256	30.88366439375122	27.72880396621322	20.9513808785553
47	20.127311660263064	27.059604799804397	29.76871469324242	23.044368846690116
48	21.244698163122006	24.599048746080896	31.559047714023432	22.59720537677367
49	20.324365013333814	24.67424150431386	31.993228556292898	23.00816492605943
50	18.42099712723528	28.37912303374111	30.453722358931156	22.746157480092453
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1299.0
1	4368.5
2	7438.0
3	56761.5
4	106085.0
5	73687.0
6	41289.0
7	39528.0
8	37767.0
9	37076.0
10	36385.0
11	35196.0
12	34007.0
13	31816.5
14	29626.0
15	27995.5
16	26365.0
17	25387.5
18	24410.0
19	22831.5
20	21253.0
21	22210.5
22	23168.0
23	23400.0
24	23632.0
25	33200.5
26	42769.0
27	49087.0
28	55405.0
29	68187.0
30	80969.0
31	94292.0
32	107615.0
33	119874.0
34	132133.0
35	145397.5
36	158662.0
37	169820.0
38	180978.0
39	209831.5
40	238685.0
41	286578.0
42	334471.0
43	363733.5
44	392996.0
45	405038.0
46	417080.0
47	398843.5
48	380607.0
49	367278.0
50	353949.0
51	325053.0
52	296157.0
53	281768.0
54	267379.0
55	248077.5
56	228776.0
57	219427.0
58	210078.0
59	197552.0
60	185026.0
61	167052.5
62	149079.0
63	125375.0
64	101671.0
65	81412.5
66	61154.0
67	47273.0
68	33392.0
69	27792.0
70	22192.0
71	18995.5
72	15799.0
73	12938.0
74	10077.0
75	7913.0
76	5749.0
77	4685.0
78	3621.0
79	2745.5
80	1870.0
81	1618.0
82	1366.0
83	981.5
84	597.0
85	401.0
86	205.0
87	150.0
88	95.0
89	89.5
90	84.0
91	54.5
92	25.0
93	17.5
94	10.0
95	6.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4883449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.86076096664491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.54199251123369	12.457357690646923
2	9.177578976725316	2.911267728046917
3	3.2610090184107965	1.5516625365326102
4	1.5740415394687068	0.9986198643633173
5	0.886330747241602	0.7028940059693409
6	0.5975105993098251	0.5686183674413928
7	0.42776586519192095	0.47492844962493763
8	0.31282605527527774	0.39693274294877023
9	0.2421340653964277	0.3456387479821236
>10	2.3549353258109704	8.629171079085596
>50	0.966353659542174	11.19413892244684
>100	1.6251933338463562	54.961495468717104
>500	0.029437153538707597	2.7953293036368687
>1k	0.0026283172802417498	0.7193111197413193
>5k	1.3141586401208748E-4	0.2042251623073152
>10k+	1.3141586401208748E-4	1.0884088105086125
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52218	1.069285253106974	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	9798	0.20063688593860612	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07576612349181899	0.0
2	0.0	0.0	0.0	0.2349978468086797	0.0
3	0.0	0.0	0.0	0.3245247365130669	0.0
4	0.0	0.0	0.0	0.4495593176052417	0.0
5	0.0	0.0	0.0	0.7439004687056218	0.0
6	0.0	0.0	0.0	0.99218810312138	0.0
7	0.0	0.0	0.0	1.172593386354603	0.0
8	0.0	0.0	0.0	1.6184667844386211	0.0
9	0.0	0.0	0.0	1.8112608527292904	0.0
10	0.0	0.0	0.0	2.1598669301143514	0.0
11	0.0	0.0	0.0	2.469729897865218	0.0
12	0.0	0.0	0.0	2.7801457535442675	0.0
13	0.0	0.0	0.0	2.8993647727251783	0.0
14	0.0	0.0	0.0	2.9425924177768623	0.0
15	0.0	0.0	0.0	3.0132392086003152	0.0
16	0.0	0.0	0.0	3.1581777551070975	0.0
17	0.0	0.0	0.0	3.3519752126007663	0.0
18	0.0	0.0	0.0	3.6120782668151135	0.0
19	0.0	0.0	0.0	3.7406349487831245	0.0
20	0.0	0.0	0.0	3.890467577320865	0.0
21	0.0	0.0	0.0	4.062825269599417	0.0
22	0.0	0.0	0.0	4.252916330241188	0.0
23	0.0	0.0	0.0	4.4440517347473065	0.0
24	0.0	0.0	0.0	4.593802453962353	0.0
25	0.0	0.0	0.0	4.7239154130615475	0.0
26	0.0	0.0	0.0	4.850055770010089	0.0
27	0.0	0.0	0.0	4.97767049476712	0.0
28	0.0	0.0	0.0	5.115155292908762	0.0
29	0.0	0.0	0.0	5.265049353438522	0.0
30	0.0	0.0	0.0	5.448935782886235	0.0
31	0.0	0.0	0.0	5.61836521687848	0.0
32	0.0	0.0	0.0	5.775467297805301	0.0
33	0.0	0.0	0.0	5.935210954388999	0.0
34	0.0	0.0	0.0	6.100524444915878	0.0
35	0.0	0.0	0.0	6.286049060817468	0.0
36	0.0	0.0	0.0	6.463403221780344	0.0
37	0.0	0.0	0.0	6.64004067616965	0.0
38	2.0477330673464594E-5	0.0	0.0	6.823742809641301	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34305	0.0	43.044453	1
CGATACG	60	3.6379788E-12	40.333332	10
CGACGGT	835	0.0	40.311375	28
TATTACG	110	0.0	40.0	1
CGGTCTA	825	0.0	40.0	31
GACCGAT	1915	0.0	38.7154	9
GTCGAAC	40	4.1282146E-7	38.5	40
TATCGCG	200	0.0	38.5	1
CTAGACG	190	0.0	38.210526	1
CGTATGG	380	0.0	38.210526	2
CTACGGG	800	0.0	37.95	3
CGCGATC	35	7.295068E-6	37.714287	20
CGACTAT	35	7.295068E-6	37.714287	21
TCGACTA	35	7.295068E-6	37.714287	27
ACGGGAG	2810	0.0	37.50178	5
TACGGGA	2665	0.0	37.478424	4
GCGGCTA	1380	0.0	37.304348	24
TTAGCGG	1210	0.0	37.272728	2
TAGGGAC	5150	0.0	37.250484	5
CAATACG	65	1.0913936E-11	37.23077	1
>>END_MODULE