FastQCFastQC Report
Thu 26 May 2016
SRR1545854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545854_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences258649
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24130.9329245425267448No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6630.25633194019694644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATC207.8451546E-444.020
ATCTAGG207.8451546E-444.02
CCTAGTG254.4332453E-544.040
TGGACGA207.8451546E-444.035
GTTTAAG302.5200825E-644.01
CGCATGG254.4332453E-544.02
GTTAGCG254.4332453E-544.01
GATCAAT207.8451546E-444.09
AGGGTGT254.4332453E-544.06
CACGAGA207.8451546E-444.013
TAACGGG502.7284841E-1144.03
GCGCATG207.8451546E-444.01
ACGGGAA1000.044.05
TCGGAGA207.8451546E-444.033
TACGATG254.4332453E-544.01
TAGGGCG600.044.05
GCGATCT207.8451546E-444.09
TTAACGG302.5200825E-644.02
TCGTAAG207.8451546E-444.01
CGATGTA254.4332453E-544.010
Produced by FastQC (version 0.11.5)