##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545854_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	258649
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51405572803297	31.0	31.0	33.0	30.0	34.0
2	31.93186905806711	31.0	31.0	34.0	30.0	34.0
3	32.01981449763966	33.0	31.0	34.0	30.0	34.0
4	35.77366624266864	37.0	35.0	37.0	35.0	37.0
5	35.68379154761859	37.0	35.0	37.0	33.0	37.0
6	35.73228583911015	37.0	35.0	37.0	33.0	37.0
7	35.98275268800575	37.0	35.0	37.0	35.0	37.0
8	35.959450838781514	37.0	35.0	37.0	35.0	37.0
9	37.74952155237407	39.0	38.0	39.0	35.0	39.0
10	37.3077607104609	39.0	37.0	39.0	34.0	39.0
11	37.0427103913025	39.0	37.0	39.0	33.0	39.0
12	36.22432331074158	38.0	35.0	39.0	32.0	39.0
13	35.89010976265131	38.0	35.0	39.0	32.0	39.0
14	36.91580481656608	39.0	35.0	41.0	32.0	41.0
15	37.11598343701309	39.0	35.0	41.0	32.0	41.0
16	37.27897652803606	39.0	35.0	41.0	33.0	41.0
17	37.19939377302831	39.0	35.0	41.0	32.0	41.0
18	37.125765032921066	38.0	35.0	41.0	32.0	41.0
19	37.008018588898466	38.0	35.0	41.0	32.0	41.0
20	36.822651547077314	38.0	35.0	41.0	32.0	41.0
21	36.67972425951773	38.0	35.0	41.0	32.0	41.0
22	36.565005084110126	38.0	35.0	40.0	32.0	41.0
23	36.49699399572393	38.0	35.0	40.0	31.0	41.0
24	36.54795108428797	38.0	35.0	40.0	32.0	41.0
25	36.5216644951266	38.0	35.0	40.0	32.0	41.0
26	36.297928853388186	38.0	35.0	40.0	31.0	41.0
27	36.2676909634292	38.0	35.0	40.0	31.0	41.0
28	36.26893589381749	37.0	35.0	40.0	31.0	41.0
29	36.26892042884372	37.0	35.0	40.0	31.0	41.0
30	36.174943649501834	37.0	35.0	40.0	31.0	41.0
31	35.9752985706498	37.0	35.0	40.0	30.0	41.0
32	35.75863815441003	37.0	35.0	40.0	30.0	41.0
33	35.537349844770326	37.0	35.0	40.0	29.0	41.0
34	35.32136988737633	37.0	34.0	40.0	28.0	41.0
35	35.114290022385546	37.0	34.0	40.0	27.0	41.0
36	34.9284435663776	37.0	34.0	40.0	25.0	41.0
37	34.83360074850473	37.0	34.0	40.0	25.0	41.0
38	34.79237499468392	37.0	34.0	40.0	25.0	41.0
39	34.71445472435617	37.0	34.0	40.0	24.0	41.0
40	34.58472679190718	37.0	34.0	40.0	23.0	41.0
41	34.58549230810867	36.0	34.0	40.0	23.0	41.0
42	34.56098419093057	36.0	34.0	40.0	23.0	41.0
43	34.459077746289374	36.0	34.0	40.0	23.0	41.0
44	34.33970748002119	36.0	34.0	40.0	23.0	41.0
45	34.23419769649216	36.0	34.0	40.0	23.0	41.0
46	34.2086882222626	36.0	34.0	40.0	23.0	41.0
47	34.171286183205815	35.0	33.0	40.0	23.0	41.0
48	34.096203735564416	35.0	34.0	40.0	23.0	41.0
49	34.05339282193243	36.0	34.0	40.0	23.0	41.0
50	33.86746904105564	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	1.0
15	6.0
16	13.0
17	41.0
18	115.0
19	211.0
20	371.0
21	595.0
22	884.0
23	1305.0
24	1919.0
25	3008.0
26	4307.0
27	5534.0
28	5810.0
29	5831.0
30	6464.0
31	7616.0
32	9217.0
33	12244.0
34	22482.0
35	38893.0
36	15839.0
37	20434.0
38	32810.0
39	62696.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.661827418625244	23.03159880765052	29.553178245421403	15.753395528302836
2	32.65622523187795	24.85491921484328	29.265143109001002	13.223712444277766
3	28.080526118407573	24.075871161303542	34.40840675974003	13.435195960548851
4	25.591825214866482	26.67669312465929	33.21799040398378	14.513491256490457
5	22.770627375323315	31.17622724232454	32.041492524618306	14.01165285773384
6	20.653859090891515	40.561533197499315	28.560713553889634	10.223894157719535
7	85.12424173300495	4.094738429299939	9.127427517601074	1.6535923200940268
8	87.37362216749341	2.9062551952646247	8.43421006847117	1.2859125687708053
9	82.4066592177043	5.673712250965594	9.802860246898307	2.1167682844317977
10	45.09315713573221	32.355818116443515	14.237054850395712	8.31396989742856
11	44.83450544947013	19.77660845392791	23.785129654473824	11.603756442128136
12	40.89557663087814	21.26201918429996	24.65696755061879	13.18543663420311
13	21.7495524823216	37.47781742825219	26.541761228537514	14.230868860888696
14	14.756291344640807	39.97889031080731	29.96879941542399	15.296018929127891
15	14.25677269194932	23.867480639785967	47.66111602983194	14.21463063843278
16	17.380697392992047	19.345135685813595	46.01332307490074	17.260843846293625
17	17.2013036972886	18.73233610027489	31.78245421401204	32.283905988424465
18	20.728477589319887	22.757868771965096	36.507003699994975	20.00664993872004
19	27.959126074332396	22.199583218956963	29.260503616870743	20.580787089839898
20	29.368758433243507	22.627962992317773	28.039930562267784	19.963348012170933
21	22.45398203743297	26.48183445518831	30.96010423392319	20.104079273455532
22	22.849885365881946	23.829591454055496	28.885478003007936	24.43504517705462
23	19.255438837961872	29.02272964519484	29.120158979930334	22.601672536912957
24	18.697539909297927	23.140626872711668	40.18612095929232	17.97571225869808
25	16.735034738197324	25.54968316134994	37.57254039257836	20.14274170787438
26	16.810813109658262	32.86113613429783	30.078214104829325	20.24983665121458
27	17.353633688898856	32.088660694609295	31.434878928586617	19.12282668790523
28	15.123197847275652	27.24425766192794	40.197719689617976	17.43482480117843
29	16.817385723509467	23.78126341103194	38.56693820583107	20.834412659627528
30	19.11857382011916	28.75479897467224	33.27714392864461	18.84948327656399
31	26.85183395257666	26.099076354441735	27.771613267400998	19.277476425580613
32	28.610588094289945	26.19766556220979	28.645770909611095	16.545975433889172
33	26.640737060649762	26.61831284868683	28.240588596901596	18.500361493761815
34	19.35093505097642	26.808145401683365	31.40510885408411	22.435810693256112
35	19.307633124427312	25.485116895870462	33.75462499371735	21.452624985984865
36	27.950620338760253	25.057896995542222	27.888373819345908	19.103108846351617
37	20.617516402537802	31.53385476069886	29.328936125792097	18.51969271097124
38	20.288885709977613	31.948316057668887	26.389044612583074	21.373753619770422
39	20.88428720002784	30.535590704004267	28.621413575927225	19.958708520040673
40	23.27323902276831	25.925868648245302	26.86923204806514	23.93166028092125
41	17.464208251336753	25.77779152442113	28.513158759554454	28.244841464687664
42	21.596062617678786	25.8423577899006	26.326798093168733	26.23478149925188
43	21.82030473730809	27.74029669552173	26.64692305015678	23.792475517013404
44	20.320975530545258	29.35290683513178	29.05017997363222	21.275937660690744
45	17.546559236648896	35.65179065065011	24.594334406860263	22.20731570584073
46	20.527046305997704	32.33610027488991	27.38034943108228	19.75650398803011
47	21.531109727855124	26.99836457902408	28.753252477295486	22.71727321582531
48	21.817211742554584	24.108734230559563	32.53250544173765	21.541548585148213
49	20.51738069739299	24.731972673391354	32.59977807762643	22.15086855158922
50	19.039702453904713	31.93323770824554	28.579658146754866	20.447401691094882
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	74.0
1	209.5
2	345.0
3	2277.5
4	4210.0
5	2983.0
6	1756.0
7	1762.0
8	1768.0
9	1824.0
10	1880.0
11	1835.5
12	1791.0
13	1673.0
14	1555.0
15	1444.5
16	1334.0
17	1247.0
18	1160.0
19	1049.5
20	939.0
21	1020.5
22	1102.0
23	1057.5
24	1013.0
25	1608.0
26	2203.0
27	2432.0
28	2661.0
29	3240.0
30	3819.0
31	4697.5
32	5576.0
33	6129.0
34	6682.0
35	7596.5
36	8511.0
37	8947.0
38	9383.0
39	10804.5
40	12226.0
41	15988.0
42	19750.0
43	22946.5
44	26143.0
45	25456.5
46	24770.0
47	23299.5
48	21829.0
49	20732.5
50	19636.0
51	18368.5
52	17101.0
53	15815.0
54	14529.0
55	12514.0
56	10499.0
57	9609.5
58	8720.0
59	8562.0
60	8404.0
61	7339.5
62	6275.0
63	5091.0
64	3907.0
65	3275.5
66	2644.0
67	2146.5
68	1649.0
69	1424.5
70	1200.0
71	955.5
72	711.0
73	576.5
74	442.0
75	319.0
76	196.0
77	166.5
78	137.0
79	109.0
80	81.0
81	51.5
82	22.0
83	12.0
84	2.0
85	4.5
86	7.0
87	4.5
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	258649.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.303016829757702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.72395562754026	17.89529439510688
2	5.6260122849372	2.8471016706037915
3	3.008587232221984	2.2837900011212104
4	2.1177764874858664	2.143445364180801
5	1.7739816031537452	2.244354318013988
6	1.4393545824038139	2.1852007933531543
7	1.2590532652874127	2.2300492172790154
8	1.1658466522018152	2.3599549969263367
9	1.0604162210066315	2.414855653801097
>10	11.695137976346912	60.119312272616554
>50	0.12223818109586529	1.9509064407749497
>100	0.0045839317910949485	0.13647839349852503
>500	0.001527977263698316	0.25633194019694644
>1k	0.001527977263698316	0.9329245425267448
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2413	0.9329245425267448	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	663	0.25633194019694644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025517206716438107	0.0
2	0.0	0.0	0.0	0.08041786359119889	0.0
3	0.0	0.0	0.0	0.11676055194491376	0.0
4	0.0	0.0	0.0	0.15310324029862865	0.0
5	0.0	0.0	0.0	0.2501459506899311	0.0
6	0.0	0.0	0.0	0.35646764534175657	0.0
7	0.0	0.0	0.0	0.43611226024457855	0.0
8	0.0	0.0	0.0	0.6182123263573415	0.0
9	0.0	0.0	0.0	0.7090690472416287	0.0
10	0.0	0.0	0.0	0.8474805624610959	0.0
11	0.0	0.0	0.0	0.9750665960432865	0.0
12	0.0	0.0	0.0	1.1208239738023345	0.0
13	0.0	0.0	0.0	1.168765392481703	0.0
14	0.0	0.0	0.0	1.1877099853469373	0.0
15	0.0	0.0	0.0	1.2225061763238985	0.0
16	0.0	0.0	0.0	1.2874590661475591	0.0
17	0.0	0.0	0.0	1.3736762949015848	0.0
18	0.0	0.0	0.0	1.5004890797953985	0.0
19	0.0	0.0	0.0	1.560415853144609	0.0
20	0.0	0.0	0.0	1.6261419916566466	0.0
21	0.0	0.0	0.0	1.6992139927082648	0.0
22	0.0	0.0	0.0	1.7815649780204061	0.0
23	0.0	0.0	0.0	1.8643025876767356	0.0
24	0.0	0.0	0.0	1.9373745887283538	0.0
25	0.0	0.0	0.0	1.992661869947303	0.0
26	0.0	0.0	0.0	2.0541351406732677	0.0
27	0.0	0.0	0.0	2.10362305672939	0.0
28	0.0	0.0	0.0	2.1778549308135737	0.0
29	0.0	0.0	0.0	2.2528600535861343	0.0
30	0.0	0.0	0.0	2.340237155372725	0.0
31	0.0	0.0	0.0	2.426454384126751	0.0
32	0.0	0.0	0.0	2.4979798878016153	0.0
33	0.0	0.0	0.0	2.574144883606741	0.0
34	0.0	0.0	0.0	2.658042366295636	0.0
35	0.0	0.0	0.0	2.7651373096358385	0.0
36	0.0	0.0	0.0	2.85483415748756	0.0
37	0.0	0.0	0.0	2.9352520210787594	0.0
38	0.0	0.0	0.0	3.0187628794234658	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATC	20	7.8451546E-4	44.0	20
ATCTAGG	20	7.8451546E-4	44.0	2
CCTAGTG	25	4.4332453E-5	44.0	40
TGGACGA	20	7.8451546E-4	44.0	35
GTTTAAG	30	2.5200825E-6	44.0	1
CGCATGG	25	4.4332453E-5	44.0	2
GTTAGCG	25	4.4332453E-5	44.0	1
GATCAAT	20	7.8451546E-4	44.0	9
AGGGTGT	25	4.4332453E-5	44.0	6
CACGAGA	20	7.8451546E-4	44.0	13
TAACGGG	50	2.7284841E-11	44.0	3
GCGCATG	20	7.8451546E-4	44.0	1
ACGGGAA	100	0.0	44.0	5
TCGGAGA	20	7.8451546E-4	44.0	33
TACGATG	25	4.4332453E-5	44.0	1
TAGGGCG	60	0.0	44.0	5
GCGATCT	20	7.8451546E-4	44.0	9
TTAACGG	30	2.5200825E-6	44.0	2
TCGTAAG	20	7.8451546E-4	44.0	1
CGATGTA	25	4.4332453E-5	44.0	10
>>END_MODULE