FastQCFastQC Report
Thu 26 May 2016
SRR1545853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545853_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1448888
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150521.0388656680157473No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32950.22741578369066484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCC650.044.0000045
AACGTAC302.5281752E-644.043
TAATACG551.8189894E-1244.01
TTATCCG254.443417E-544.026
TACGAAC207.857162E-444.030
GTCAACG551.8189894E-1244.01
TGTACCG351.4464422E-744.01
ATCGCTA302.5281752E-644.019
TACGTAA408.314601E-944.016
GTACGAT207.857162E-444.019
CGTTTTT93800.042.921111
CGACGGT1550.042.58064728
CGGTCTA1550.042.58064731
GCGCGAC2200.040.09
ATAGCGG2050.039.707322
AATACGG1350.039.1111152
GTTCGAT850.038.82353239
CATACGA2100.038.761918
CGCAAGG1650.038.6666682
GGGCGAT18450.038.5149047
Produced by FastQC (version 0.11.5)