##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545853_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1448888
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47717490930976	31.0	31.0	33.0	30.0	34.0
2	31.882633440265913	31.0	31.0	34.0	30.0	34.0
3	31.94412818658171	31.0	31.0	34.0	30.0	34.0
4	35.704425048727025	37.0	35.0	37.0	33.0	37.0
5	35.62531955541077	37.0	35.0	37.0	33.0	37.0
6	35.674984539867815	37.0	35.0	37.0	33.0	37.0
7	35.97487728520079	37.0	35.0	37.0	35.0	37.0
8	35.9594261254148	37.0	35.0	37.0	35.0	37.0
9	37.712486403365894	39.0	38.0	39.0	35.0	39.0
10	37.23221532651247	39.0	37.0	39.0	34.0	39.0
11	36.991559734085726	39.0	37.0	39.0	33.0	39.0
12	36.358184345511866	38.0	35.0	39.0	32.0	39.0
13	36.07506377304526	38.0	35.0	39.0	32.0	39.0
14	37.1228562870284	39.0	35.0	41.0	32.0	41.0
15	37.26499080674282	39.0	35.0	41.0	32.0	41.0
16	37.37658811447123	39.0	35.0	41.0	32.0	41.0
17	37.27855569236546	39.0	35.0	41.0	32.0	41.0
18	37.15979565018138	39.0	35.0	41.0	32.0	41.0
19	37.05249198005643	38.0	35.0	41.0	32.0	41.0
20	36.855057119666945	38.0	35.0	41.0	32.0	41.0
21	36.73280750478988	38.0	35.0	41.0	32.0	41.0
22	36.63478405508224	38.0	35.0	41.0	31.0	41.0
23	36.5688044900641	38.0	35.0	40.0	31.0	41.0
24	36.60773365505132	38.0	35.0	41.0	31.0	41.0
25	36.57436944746592	38.0	35.0	41.0	31.0	41.0
26	36.32872382130296	38.0	35.0	40.0	31.0	41.0
27	36.32901438896588	38.0	35.0	40.0	31.0	41.0
28	36.30500356135188	38.0	35.0	40.0	31.0	41.0
29	36.28801467056115	38.0	35.0	40.0	31.0	41.0
30	36.17202709940313	38.0	35.0	40.0	30.0	41.0
31	35.98116348537637	38.0	35.0	40.0	30.0	41.0
32	35.77055714451359	38.0	35.0	40.0	30.0	41.0
33	35.487929363760344	38.0	35.0	40.0	28.0	41.0
34	35.202150200705645	38.0	34.0	40.0	26.0	41.0
35	34.9473692928646	38.0	34.0	40.0	24.0	41.0
36	34.799100413558534	38.0	34.0	40.0	24.0	41.0
37	34.72532176400108	37.0	34.0	40.0	23.0	41.0
38	34.65062447891072	37.0	34.0	40.0	23.0	41.0
39	34.54987065942985	37.0	34.0	40.0	23.0	41.0
40	34.4501507362888	37.0	34.0	40.0	23.0	41.0
41	34.40687893060057	37.0	34.0	40.0	23.0	41.0
42	34.37304470738939	37.0	34.0	40.0	23.0	41.0
43	34.27127424618052	37.0	33.0	40.0	23.0	41.0
44	34.155764972861945	37.0	33.0	40.0	23.0	41.0
45	34.067257786661216	36.0	33.0	40.0	23.0	41.0
46	34.02245515181298	36.0	33.0	40.0	22.0	41.0
47	33.96747712728658	36.0	33.0	40.0	22.0	41.0
48	33.86229715478353	36.0	33.0	40.0	22.0	41.0
49	33.81301936381556	36.0	33.0	40.0	21.0	41.0
50	33.661504546935305	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	6.0
14	13.0
15	39.0
16	105.0
17	272.0
18	616.0
19	1236.0
20	2284.0
21	3597.0
22	5696.0
23	8378.0
24	11807.0
25	18002.0
26	26536.0
27	33161.0
28	34837.0
29	35515.0
30	38190.0
31	43521.0
32	51748.0
33	67121.0
34	116580.0
35	188458.0
36	93379.0
37	118731.0
38	190543.0
39	358514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.661039362600835	22.934484929131855	28.88104532579468	16.52343038247263
2	33.96170028325171	23.85284438824809	29.664266665194273	12.52118866330593
3	28.020799399263435	24.61777583912628	34.454975125751616	12.90644963585867
4	25.07585127352839	26.47478618085042	34.36290451711933	14.086458028501859
5	23.09971509184975	31.324712469148754	32.13395376316181	13.441618675839678
6	20.40751251994633	40.066727034801865	29.221651362976296	10.30410908227551
7	83.88916189519135	4.241183583548211	10.230811491295395	1.6388430299650492
8	84.95252911198105	3.0545494199689696	10.141225546764138	1.8516959212858415
9	79.83557045127021	6.169282925940445	11.375482438946282	2.6196641838430574
10	40.419894429383085	33.7705191843676	16.245009966263783	9.564576419985533
11	39.905430923577256	20.20066423353634	26.419088293919195	13.474816548967208
12	36.4131665111451	20.724997377299005	28.316543445732172	14.545292665823723
13	21.99652423099646	32.521561362921084	29.007487121157745	16.474427284924715
14	16.218575900966812	35.399009447245064	31.833033333149285	16.549381318638844
15	16.393882756983285	23.513756756905984	45.059314453567154	15.033046032543579
16	19.249037882845325	20.207290004472394	42.80027165660838	17.7434004560739
17	18.81574007100618	20.602558651876475	32.16756574697285	28.414135530144495
18	21.39875545935918	22.655857457581263	36.30915571113847	19.636231371921088
19	26.69219428968975	23.438043520272096	30.09114576143912	19.778616428599037
20	28.73921241669473	22.803142824014003	28.908307612458657	19.549337146832606
21	22.284262137584133	26.528896643494875	31.24506518102158	19.94177603789941
22	22.45963801204786	23.056785617659887	31.031522105228287	23.452054265063964
23	20.32896952697517	27.2443418676944	30.591322448664076	21.835366156666353
24	20.311438841373523	22.24851058190833	38.64825990690792	18.791790669810226
25	18.73871548387453	24.42997664415745	36.33821247743097	20.493095394537054
26	17.924228787870423	30.740678368514335	31.264252309357243	20.070840534257997
27	18.354351751136043	29.656536599102207	32.08011937430636	19.908992275455383
28	16.229825907868655	27.243720701669144	38.34485481279437	18.18159857766784
29	17.966330040693276	23.582223056578563	37.707952581566	20.74349432116216
30	19.578946060703103	26.956949053342978	33.506937734317624	19.95716715163629
31	25.910560374576917	26.149226165169427	28.88767109673073	19.052542363522925
32	27.659970957037398	25.882814958782184	29.420976638635977	17.036237445544444
33	26.250131135049777	26.52378927839833	28.534917812833015	18.69116177371888
34	20.28252011197553	27.13211787246495	31.397319875656365	21.188042139903153
35	20.320756331752353	26.088489931588914	32.79314895285212	20.797604783806616
36	26.305207855955743	26.492178829557567	28.577709250128375	18.624904064358322
37	21.342436406402705	30.71721209644914	29.39047048495122	18.549881012196938
38	21.090726129279833	30.69457404575095	27.49039263214272	20.7243071928265
39	21.708786324408788	29.54762548934079	28.718782956308566	20.024805229941858
40	23.002951228804434	26.4844487634655	28.650523711977737	21.862076295752328
41	18.58335495911347	26.87184930788301	28.6995958279729	25.84519990503062
42	20.9439929104251	26.213827431795973	27.50661196724661	25.335567690532322
43	21.226554433468976	26.47402697793066	28.196451347516167	24.102967241084198
44	20.679583239008124	28.206251967025747	29.443959781570417	21.670205012395712
45	18.517649397330917	32.4700045828249	26.380714037247877	22.63163198259631
46	21.200948589539014	31.11185957782796	27.734786953857025	19.952404878776
47	21.323663388750543	27.219909337367692	28.710983871769248	22.745443402112517
48	20.864828751428682	24.572982866860656	32.302979940478494	22.259208441232172
49	20.826385476310108	24.545720580196676	32.965418997189566	21.662474946303647
50	19.03549480705203	29.291843123830137	30.173898879692562	21.49876318942527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	440.0
1	1437.0
2	2434.0
3	15530.5
4	28627.0
5	20328.0
6	12029.0
7	11873.5
8	11718.0
9	11727.5
10	11737.0
11	11378.0
12	11019.0
13	10328.0
14	9637.0
15	8819.5
16	8002.0
17	7409.0
18	6816.0
19	6478.5
20	6141.0
21	6700.0
22	7259.0
23	6941.0
24	6623.0
25	8356.0
26	10089.0
27	13055.5
28	16022.0
29	19936.5
30	23851.0
31	27241.5
32	30632.0
33	34812.5
34	38993.0
35	41592.0
36	44191.0
37	49820.0
38	55449.0
39	64140.5
40	72832.0
41	87175.5
42	101519.0
43	118087.5
44	134656.0
45	132728.0
46	130800.0
47	120858.5
48	110917.0
49	107545.0
50	104173.0
51	95470.0
52	86767.0
53	81148.0
54	75529.0
55	69097.0
56	62665.0
57	59366.5
58	56068.0
59	54260.0
60	52452.0
61	47945.5
62	43439.0
63	35999.5
64	28560.0
65	23168.5
66	17777.0
67	14136.5
68	10496.0
69	9058.0
70	7620.0
71	6018.5
72	4417.0
73	3442.0
74	2467.0
75	2153.0
76	1839.0
77	1524.0
78	1209.0
79	881.0
80	553.0
81	393.0
82	233.0
83	180.0
84	127.0
85	80.0
86	33.0
87	26.5
88	20.0
89	11.5
90	3.0
91	3.0
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1448888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.716665978392566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16601760000765	15.004405742372832
2	6.579001043736961	2.4627393001424154
3	2.171521016506456	1.2193090059303247
4	1.10256147866901	0.8254509966756186
5	0.6955577549955795	0.6509261084466437
6	0.5533826474608382	0.6214486902457848
7	0.42455274685983585	0.5562348367229221
8	0.35844051625958057	0.5367049132762527
9	0.3065897955811529	0.5164504916648487
>10	4.706346684194633	21.955032199538145
>50	1.7959611167799896	24.165689390936578
>100	1.1378277804827956	30.001222508472207
>500	0.0011199092327586568	0.12325048616969064
>1k	7.466061551724379E-4	0.3094537433364852
>5k	0.0	0.0
>10k+	3.7330307758621893E-4	1.0516815860692652
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15052	1.0388656680157473	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3295	0.22741578369066484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.039754625616334735	0.0
2	0.0	0.0	0.0	0.1375537653704082	0.0
3	0.0	0.0	0.0	0.1972547222421609	0.0
4	0.0	0.0	0.0	0.28483913180314835	0.0
5	0.0	0.0	0.0	0.4861659424330935	0.0
6	0.0	0.0	0.0	0.6605065401880614	0.0
7	0.0	0.0	0.0	0.7807366752985738	0.0
8	0.0	0.0	0.0	1.1230681736614563	0.0
9	0.0	0.0	0.0	1.2509593564167831	0.0
10	0.0	0.0	0.0	1.4874855751445246	0.0
11	0.0	0.0	0.0	1.6845332420449337	0.0
12	0.0	0.0	0.0	1.8779919496883126	0.0
13	0.0	0.0	0.0	1.9539812601112025	0.0
14	0.0	0.0	0.0	1.981243546775182	0.0
15	0.0	0.0	0.0	2.0306607550065983	0.0
16	0.0	0.0	0.0	2.1444721745228064	0.0
17	0.0	0.0	0.0	2.276987593243922	0.0
18	0.0	0.0	0.0	2.4671334154192732	0.0
19	0.0	0.0	0.0	2.5577546366592863	0.0
20	0.0	0.0	0.0	2.651895798709079	0.0
21	0.0	0.0	0.0	2.7670185687230484	0.0
22	0.0	0.0	0.0	2.8906996261960898	0.0
23	0.0	0.0	0.0	3.030323944984015	0.0
24	0.0	0.0	0.0	3.134955911015896	0.0
25	0.0	0.0	0.0	3.225163021572406	0.0
26	0.0	0.0	0.0	3.3124023388971406	0.0
27	0.0	0.0	0.0	3.402402394111898	0.0
28	0.0	0.0	0.0	3.494818094980426	0.0
29	0.0	0.0	0.0	3.600554356168317	0.0
30	0.0	0.0	0.0	3.731137258366416	0.0
31	0.0	0.0	0.0	3.859166478016244	0.0
32	0.0	0.0	0.0	3.972080657718195	0.0
33	6.901844725058113E-5	0.0	0.0	4.082648210213626	0.0
34	6.901844725058113E-5	0.0	0.0	4.19956545985611	0.0
35	6.901844725058113E-5	0.0	0.0	4.343744996162575	0.0
36	6.901844725058113E-5	0.0	0.0	4.476191396436439	0.0
37	6.901844725058113E-5	0.0	0.0	4.606222151056534	0.0
38	6.901844725058113E-5	0.0	0.0	4.734527444495365	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGCC	65	0.0	44.000004	5
AACGTAC	30	2.5281752E-6	44.0	43
TAATACG	55	1.8189894E-12	44.0	1
TTATCCG	25	4.443417E-5	44.0	26
TACGAAC	20	7.857162E-4	44.0	30
GTCAACG	55	1.8189894E-12	44.0	1
TGTACCG	35	1.4464422E-7	44.0	1
ATCGCTA	30	2.5281752E-6	44.0	19
TACGTAA	40	8.314601E-9	44.0	16
GTACGAT	20	7.857162E-4	44.0	19
CGTTTTT	9380	0.0	42.92111	1
CGACGGT	155	0.0	42.580647	28
CGGTCTA	155	0.0	42.580647	31
GCGCGAC	220	0.0	40.0	9
ATAGCGG	205	0.0	39.70732	2
AATACGG	135	0.0	39.111115	2
GTTCGAT	85	0.0	38.823532	39
CATACGA	210	0.0	38.7619	18
CGCAAGG	165	0.0	38.666668	2
GGGCGAT	1845	0.0	38.514904	7
>>END_MODULE