FastQCFastQC Report
Thu 26 May 2016
SRR1545852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545852_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2463809
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251751.0217918677949467No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA50420.20464248649144476No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA31090.12618672957197574No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAT207.858237E-444.042
CGTGCTA207.858237E-444.031
ACGTTAC454.802132E-1044.030
GCACGAC408.3200575E-944.09
CCGTCGA254.4443288E-544.013
CATACGT207.858237E-444.022
TCCCGTA254.4443288E-544.042
ACCGTAA207.858237E-444.010
TTCGATC207.858237E-444.012
TATCGTG551.8189894E-1244.01
CGTATCT351.4469879E-743.99999634
CGTTTTT166850.043.0506481
TATAGCG1750.041.4857141
CGTATGG1600.041.252
CGTAAGG4450.041.0337072
CTAACGG1400.040.857142
CGTTGAT4150.040.8192825
TCTCGAG1350.040.740741
GATCTCG603.6379788E-1240.3333329
TTATCGG1050.039.809522
Produced by FastQC (version 0.11.5)