FastQCFastQC Report
Thu 26 May 2016
SRR1545851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545851_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2242237
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218650.9751422351874489No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA56340.25126692673432827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG254.4441982E-544.015
CGTATCT408.3182385E-944.025
CTCCGTA351.4469151E-744.035
CGTAGCA302.528799E-644.033
CGAATCT254.4441982E-544.044
CGTTTTT148650.042.8456081
CGGTCTA2300.040.17391631
CGGGATA6700.040.0597046
ACGTAAC557.8216544E-1140.039
GCGTAAC1050.039.80952532
GCATAAC501.3496901E-939.6000029
GCGTATG501.3496901E-939.6000021
GCGCGAC3450.039.5362329
AAGTACG452.3526809E-839.111111
CGACGGT2250.039.1111128
TAGTACG900.039.111111
TACGGGA11650.039.090134
TATGGGA21600.039.009264
CTCACGA2500.038.7224
CTATGCG1600.038.51
Produced by FastQC (version 0.11.5)