##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545851_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2242237
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50337676168933	31.0	31.0	33.0	30.0	34.0
2	31.915873299744852	31.0	31.0	34.0	30.0	34.0
3	31.9790704550857	33.0	31.0	34.0	30.0	34.0
4	35.734476328773454	37.0	35.0	37.0	33.0	37.0
5	35.65325119512344	37.0	35.0	37.0	33.0	37.0
6	35.69509199964143	37.0	35.0	37.0	33.0	37.0
7	35.981510875076985	37.0	35.0	37.0	35.0	37.0
8	35.972625998054625	37.0	35.0	37.0	35.0	37.0
9	37.74858589881444	39.0	38.0	39.0	35.0	39.0
10	37.27057844465148	39.0	37.0	39.0	34.0	39.0
11	37.010486848624836	39.0	37.0	39.0	33.0	39.0
12	36.385611333681496	38.0	35.0	39.0	32.0	39.0
13	36.10420040343639	38.0	35.0	39.0	32.0	39.0
14	37.14760482500289	39.0	35.0	41.0	32.0	41.0
15	37.31705301446725	39.0	35.0	41.0	32.0	41.0
16	37.45720055462469	39.0	35.0	41.0	33.0	41.0
17	37.378834173194	39.0	35.0	41.0	32.0	41.0
18	37.263833394953345	39.0	35.0	41.0	32.0	41.0
19	37.13071187390093	38.0	35.0	41.0	32.0	41.0
20	36.93218112090738	38.0	35.0	41.0	32.0	41.0
21	36.79960503729088	38.0	35.0	41.0	32.0	41.0
22	36.700190479418545	38.0	35.0	41.0	32.0	41.0
23	36.62903430814851	38.0	35.0	40.0	31.0	41.0
24	36.66663604248792	38.0	35.0	41.0	32.0	41.0
25	36.63568034957946	38.0	35.0	41.0	32.0	41.0
26	36.403759727450755	38.0	35.0	41.0	31.0	41.0
27	36.378918018032884	38.0	35.0	40.0	31.0	41.0
28	36.363707315506794	38.0	35.0	40.0	31.0	41.0
29	36.34096038911141	38.0	35.0	40.0	31.0	41.0
30	36.235723074768636	38.0	35.0	40.0	31.0	41.0
31	36.05803311603546	38.0	35.0	40.0	30.0	41.0
32	35.82933115455681	38.0	35.0	40.0	30.0	41.0
33	35.56885690495697	38.0	35.0	40.0	29.0	41.0
34	35.31767426904471	38.0	35.0	40.0	27.0	41.0
35	35.06030852224809	38.0	34.0	40.0	25.0	41.0
36	34.8796456396001	38.0	34.0	40.0	24.0	41.0
37	34.7934504693304	38.0	34.0	40.0	24.0	41.0
38	34.72791903799643	37.0	34.0	40.0	24.0	41.0
39	34.64213997003885	37.0	34.0	40.0	23.0	41.0
40	34.49530624996376	37.0	34.0	40.0	23.0	41.0
41	34.473292073942225	37.0	34.0	40.0	23.0	41.0
42	34.42980068565455	37.0	34.0	40.0	23.0	41.0
43	34.32981348537197	37.0	33.0	40.0	23.0	41.0
44	34.19626471242781	37.0	33.0	40.0	23.0	41.0
45	34.109873309556484	36.0	33.0	40.0	23.0	41.0
46	34.050132523903585	36.0	33.0	40.0	23.0	41.0
47	34.01286884481881	36.0	33.0	40.0	23.0	41.0
48	33.90424339621548	36.0	33.0	40.0	22.0	41.0
49	33.83828515897294	36.0	33.0	40.0	22.0	41.0
50	33.69628678859549	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	8.0
14	22.0
15	52.0
16	141.0
17	422.0
18	998.0
19	1928.0
20	3431.0
21	5440.0
22	8370.0
23	12430.0
24	18255.0
25	27411.0
26	40139.0
27	50326.0
28	52258.0
29	52791.0
30	56408.0
31	65592.0
32	79282.0
33	102408.0
34	177455.0
35	298426.0
36	145638.0
37	187178.0
38	294211.0
39	561211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.531222613845014	23.342224751442423	29.58041455921029	17.546138075502277
2	32.75505666885347	25.014394107313365	29.626752212188094	12.603797011645067
3	27.617330371410336	23.912503450794897	35.363612321088276	13.106553856706494
4	25.4055659593522	26.624527202075427	34.08814500875688	13.881761829815492
5	22.74148540051743	31.02245659134159	32.53701548944202	13.69904251869896
6	20.95652689702293	39.09956886805453	29.19990170530591	10.744002529616628
7	84.79353431416928	3.9454794475338693	9.717215441543422	1.5437707967534207
8	86.17719714731315	2.8949660539898328	9.29147989262509	1.6363569060719272
9	81.211040581348	5.60516127420964	10.76634628721228	2.4174518572300787
10	40.52011451064272	35.288151966094574	15.099563516256309	9.092170007006397
11	39.40511194846932	20.418938765170676	27.020426475881003	13.155522810479
12	36.67007546481483	20.340534921152404	27.897497008567782	15.091892605464988
13	21.59414905739224	33.91769023524275	28.30686497457673	16.181295732788282
14	15.531007649949583	36.23332413121361	31.350611019263354	16.88505719957346
15	14.91929711266026	24.162700017883925	44.992567690212944	15.925435179242871
16	17.530127279141322	20.172622251795865	43.58566913310234	18.711581335960474
17	17.51532063738133	20.470271429826553	32.456337131177484	29.55807080161464
18	20.51553872315906	23.282819791128233	35.52149036877012	20.68015111694259
19	26.556603962917393	23.35975189063422	29.964495278599006	20.119148867849386
20	28.222707947464965	22.489148114137798	29.33534679875499	19.952797139642243
21	22.122103952436785	26.02423383433598	31.19460610096078	20.659056112266455
22	22.205547406451682	23.557902219970504	29.72183582734564	24.514714546232177
23	19.443216751842023	27.84598595063769	29.920878123053008	22.789919174467286
24	18.67741902394796	23.28768100785064	39.70650738525856	18.328392582942836
25	17.360965856865267	25.695901013140006	36.008459409063356	20.934673720931375
26	17.79191940905444	31.390125129502366	30.426132473953466	20.391822987489725
27	18.408223573154846	30.777924010708947	31.445783831057998	19.368068585078206
28	15.823438824709433	27.331856534344944	38.36400880014021	18.48069584080541
29	17.008728336924243	23.523829104595098	37.974397889250774	21.49304466922988
30	18.565923227562475	27.407941265798396	34.16342697047636	19.86270853616277
31	25.41417343483316	25.62601544796558	29.280535465251887	19.67927565194937
32	26.491891802695257	25.765875774951535	29.719070731595277	18.023161690757934
33	25.075404607095503	26.744541277304762	28.458900642527972	19.72115347307176
34	20.60932898707853	25.978743549410698	30.95631728492572	22.455610178585047
35	20.61508217017202	25.10858575609982	32.367631075573186	21.908700998154966
36	26.04541803564922	25.94761392305987	28.79753567530997	19.209432365980938
37	20.124143879527452	30.351742478605072	30.111223746642303	19.41288989522517
38	19.79010247355654	30.711918499248743	28.21258412915316	21.285394898041552
39	21.148032076894637	30.038573085717523	29.12934716535317	19.68404767203467
40	22.839378709743883	26.831195810255558	28.39280593443066	21.936619545569894
41	18.27554357545612	25.570891926232598	28.766093860729264	27.387470637582023
42	21.619168714101143	26.43770484565191	26.43155027769143	25.51157616255552
43	22.16407097019628	26.682371221240214	27.633742552638278	23.519815255925224
44	19.8991899607401	29.734501749815028	29.0738222587532	21.29248603069167
45	18.231525035043127	33.29179743265319	26.139832676028448	22.336844856275228
46	20.039094886044605	31.708467927342205	28.180161151564263	20.072276035048926
47	21.495631371706022	27.34470085008855	29.170957396564233	21.98871038164119
48	22.062163812299946	24.658276533658128	31.537165785775546	21.74239386826638
49	20.23265158856981	25.58721491082343	32.53032574165889	21.649807758947873
50	18.724604044978296	30.5477074903322	29.77120616598513	20.956482298704373
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	456.0
1	1830.5
2	3205.0
3	21601.0
4	39997.0
5	28973.5
6	17950.0
7	17845.0
8	17740.0
9	17837.5
10	17935.0
11	17395.0
12	16855.0
13	15751.0
14	14647.0
15	13763.5
16	12880.0
17	11662.0
18	10444.0
19	9857.5
20	9271.0
21	10206.5
22	11142.0
23	11884.0
24	12626.0
25	14544.5
26	16463.0
27	21877.0
28	27291.0
29	32458.5
30	37626.0
31	43312.5
32	48999.0
33	57197.0
34	65395.0
35	67388.5
36	69382.0
37	73537.0
38	77692.0
39	95353.0
40	113014.0
41	137316.5
42	161619.0
43	187585.5
44	213552.0
45	215164.0
46	216776.0
47	195328.0
48	173880.0
49	167840.5
50	161801.0
51	149781.5
52	137762.0
53	123644.0
54	109526.0
55	103334.5
56	97143.0
57	89037.0
58	80931.0
59	76757.0
60	72583.0
61	65329.0
62	58075.0
63	50780.0
64	43485.0
65	34652.0
66	25819.0
67	21049.0
68	16279.0
69	14176.0
70	12073.0
71	10666.5
72	9260.0
73	6704.5
74	4149.0
75	3368.5
76	2588.0
77	2387.5
78	2187.0
79	1547.0
80	907.0
81	644.0
82	381.0
83	298.0
84	215.0
85	149.0
86	83.0
87	75.0
88	67.0
89	51.0
90	35.0
91	26.0
92	17.0
93	22.5
94	28.0
95	17.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2242237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.22740260842594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63684343941887	13.891633670034789
2	7.194560054870304	2.478871653114995
3	2.3649458754389494	1.2222562422996939
4	1.1606094698045581	0.7997714642989956
5	0.7372419091507457	0.6350381594372239
6	0.4997220536634399	0.5165347806461708
7	0.3940031551047826	0.4751355687386128
8	0.30951696217544505	0.42657386572266687
9	0.2608065776875025	0.4043717925073491
>10	3.4122813414221733	14.376922271152786
>50	1.434948247151298	17.86765839060077
>100	1.589542130650605	45.09910991452963
>500	0.003930618522144088	0.43264953942278733
>1k	5.240824696192117E-4	0.13208812107168988
>5k	2.6204123480960586E-4	0.2543350902658581
>10k+	2.6204123480960586E-4	0.9870494761560147
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21865	0.9751422351874489	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5634	0.25126692673432827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01841910556288207	0.0
2	0.0	0.0	0.0	0.09811630081922651	0.0
3	0.0	0.0	0.0	0.16184729803316955	0.0
4	0.0	0.0	0.0	0.2518467048755328	0.0
5	0.0	0.0	0.0	0.44928346111494905	0.0
6	0.0	0.0	0.0	0.6410562308979827	0.0
7	0.0	0.0	0.0	0.7655747363012919	0.0
8	0.0	0.0	0.0	1.0737045191922174	0.0
9	0.0	0.0	0.0	1.2189612427232268	0.0
10	0.0	0.0	0.0	1.4627356519404506	0.0
11	0.0	0.0	0.0	1.669894841624681	0.0
12	0.0	0.0	0.0	1.8683573591908438	0.0
13	0.0	0.0	0.0	1.9483221443585135	0.0
14	0.0	0.0	0.0	1.9779354278784982	0.0
15	0.0	0.0	0.0	2.021374190150283	0.0
16	0.0	0.0	0.0	2.123504339639387	0.0
17	0.0	0.0	0.0	2.255559960878355	0.0
18	0.0	0.0	0.0	2.4404199912854887	0.0
19	0.0	0.0	0.0	2.527921892288817	0.0
20	0.0	0.0	0.0	2.6237190805432253	0.0
21	0.0	0.0	0.0	2.7433763692241273	0.0
22	0.0	0.0	0.0	2.870258585510809	0.0
23	0.0	0.0	0.0	3.007576808339172	0.0
24	0.0	0.0	0.0	3.1050687326986397	0.0
25	0.0	0.0	0.0	3.1866390573342604	0.0
26	0.0	0.0	0.0	3.2735611801963844	0.0
27	0.0	0.0	0.0	3.3628916122604346	0.0
28	4.459831855419387E-5	0.0	0.0	3.457083261046892	0.0
29	4.459831855419387E-5	0.0	0.0	3.563093464250211	0.0
30	4.459831855419387E-5	0.0	0.0	3.688013354520508	0.0
31	4.459831855419387E-5	0.0	0.0	3.8107925255002035	0.0
32	4.459831855419387E-5	0.0	0.0	3.920548987462075	0.0
33	4.459831855419387E-5	0.0	0.0	4.019066673148289	0.0
34	4.459831855419387E-5	0.0	0.0	4.133104573691362	0.0
35	4.459831855419387E-5	0.0	0.0	4.277112544302855	0.0
36	4.459831855419387E-5	0.0	0.0	4.402478417758694	0.0
37	4.459831855419387E-5	0.0	0.0	4.533062294485373	0.0
38	4.459831855419387E-5	0.0	0.0	4.652496591573504	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTCG	25	4.4441982E-5	44.0	15
CGTATCT	40	8.3182385E-9	44.0	25
CTCCGTA	35	1.4469151E-7	44.0	35
CGTAGCA	30	2.528799E-6	44.0	33
CGAATCT	25	4.4441982E-5	44.0	44
CGTTTTT	14865	0.0	42.845608	1
CGGTCTA	230	0.0	40.173916	31
CGGGATA	670	0.0	40.059704	6
ACGTAAC	55	7.8216544E-11	40.0	39
GCGTAAC	105	0.0	39.809525	32
GCATAAC	50	1.3496901E-9	39.600002	9
GCGTATG	50	1.3496901E-9	39.600002	1
GCGCGAC	345	0.0	39.536232	9
AAGTACG	45	2.3526809E-8	39.11111	1
CGACGGT	225	0.0	39.11111	28
TAGTACG	90	0.0	39.11111	1
TACGGGA	1165	0.0	39.09013	4
TATGGGA	2160	0.0	39.00926	4
CTCACGA	250	0.0	38.72	24
CTATGCG	160	0.0	38.5	1
>>END_MODULE