FastQCFastQC Report
Thu 26 May 2016
SRR1545850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545850_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3216965
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303590.9437155828552689No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA66520.20677874953566483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTCG454.802132E-1044.013
CGTCCGA351.4471698E-744.040
TCGCGAT254.4446322E-544.028
CGTTTTT269350.043.1178781
CCGTAAG850.041.4117661
TAATGCG850.041.4117661
TATCGCG1650.041.3333321
TATTGCG1350.040.740741
TACGGGA21050.040.5510674
CGGTCTA1800.040.33333231
TATTACG1100.040.01
TATCCGA557.8216544E-1140.015
TCGGTAC1000.039.636
GCGGATA950.039.368425
CGTTAGG5100.039.2548982
CGACGTA452.3532266E-839.1111121
TCGCGAG1250.038.721
TCTGCGA404.1276144E-738.50000414
CGTTTCG1600.038.50000442
TGCGTTC404.1276144E-738.50000424
Produced by FastQC (version 0.11.5)