##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545850_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3216965
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51751946322077	31.0	31.0	33.0	30.0	34.0
2	31.921247511241184	31.0	31.0	34.0	30.0	34.0
3	31.939220973806055	31.0	31.0	34.0	30.0	34.0
4	35.697810513947154	37.0	35.0	37.0	33.0	37.0
5	35.63314583776945	37.0	35.0	37.0	33.0	37.0
6	35.691910543011815	37.0	35.0	37.0	33.0	37.0
7	36.03735881490784	37.0	35.0	37.0	35.0	37.0
8	36.035781551866435	37.0	35.0	37.0	35.0	37.0
9	37.81170419945508	39.0	38.0	39.0	35.0	39.0
10	37.30001942824992	39.0	37.0	39.0	34.0	39.0
11	37.098374399472796	39.0	37.0	39.0	33.0	39.0
12	36.57359747463836	39.0	35.0	39.0	33.0	39.0
13	36.33013290477204	39.0	35.0	39.0	32.0	39.0
14	37.404156091222625	39.0	35.0	41.0	32.0	41.0
15	37.551921143064966	40.0	35.0	41.0	33.0	41.0
16	37.649975364979106	40.0	35.0	41.0	33.0	41.0
17	37.560770788615976	39.0	35.0	41.0	33.0	41.0
18	37.41586650771768	39.0	36.0	41.0	32.0	41.0
19	37.28353681187082	38.0	36.0	41.0	32.0	41.0
20	37.08947035482201	38.0	35.0	41.0	32.0	41.0
21	36.958027519727445	38.0	35.0	41.0	32.0	41.0
22	36.85301798434239	38.0	35.0	41.0	32.0	41.0
23	36.7679906371378	38.0	35.0	41.0	32.0	41.0
24	36.830876618178934	38.0	35.0	41.0	32.0	41.0
25	36.814441251303634	38.0	35.0	41.0	32.0	41.0
26	36.584951033038905	38.0	35.0	41.0	31.0	41.0
27	36.57116132752454	38.0	35.0	41.0	31.0	41.0
28	36.557022846067646	38.0	35.0	41.0	31.0	41.0
29	36.53184414502489	38.0	35.0	41.0	31.0	41.0
30	36.393350254043796	38.0	35.0	40.0	31.0	41.0
31	36.17937061795823	38.0	35.0	40.0	30.0	41.0
32	35.914354989874	38.0	35.0	41.0	30.0	41.0
33	35.584333680969486	38.0	35.0	41.0	27.0	41.0
34	35.27719760706131	38.0	35.0	40.0	25.0	41.0
35	34.99242080656768	38.0	34.0	40.0	24.0	41.0
36	34.826063385831056	38.0	34.0	40.0	23.0	41.0
37	34.728020976292875	38.0	34.0	40.0	23.0	41.0
38	34.6443296709787	38.0	34.0	40.0	23.0	41.0
39	34.54209790905403	38.0	34.0	40.0	22.0	41.0
40	34.43989816488523	38.0	34.0	40.0	22.0	41.0
41	34.412609089623295	38.0	34.0	40.0	21.0	41.0
42	34.399424612950405	38.0	34.0	40.0	21.0	41.0
43	34.30102410190972	38.0	34.0	40.0	21.0	41.0
44	34.20111720208333	38.0	34.0	40.0	20.0	41.0
45	34.104214686824385	37.0	33.0	40.0	20.0	41.0
46	34.04950660016506	37.0	33.0	40.0	20.0	41.0
47	33.98446423880894	37.0	33.0	40.0	20.0	41.0
48	33.89145669909371	37.0	33.0	40.0	19.0	41.0
49	33.82701925572706	37.0	33.0	40.0	19.0	41.0
50	33.68214232980465	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	4.0
13	7.0
14	17.0
15	51.0
16	166.0
17	481.0
18	1232.0
19	2456.0
20	4527.0
21	7511.0
22	11637.0
23	17867.0
24	27328.0
25	43920.0
26	64827.0
27	78130.0
28	78164.0
29	75457.0
30	80096.0
31	90543.0
32	108943.0
33	138967.0
34	229477.0
35	374905.0
36	207094.0
37	269934.0
38	434622.0
39	868598.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.684760325337702	22.03399788309789	29.644027833687964	17.637213957876448
2	33.51391762111182	24.576363124870802	29.7733733503473	12.136345903670074
3	26.516048511562918	23.70411241651681	36.90012169855749	12.879717373362782
4	23.85841934867181	26.367834278582453	36.331449052134545	13.442297320611196
5	21.584350466977416	31.482686320802372	33.980195619162785	12.952767593057432
6	19.680599571335094	39.84295757025644	30.738413380313435	9.738029478095036
7	85.05056784888862	2.972926345173168	10.789703960099036	1.1868018458391683
8	86.22328809918666	2.173850197313306	10.320876975658734	1.2819847278413037
9	80.6631094836282	5.708330678139178	11.69670792190776	1.931851916324859
10	31.759375684845807	45.64000540882478	15.735359259426199	6.8652596469032146
11	32.620839828844886	21.264918953112637	32.612664421279064	13.501576796763409
12	31.008108574386107	21.52824168121195	32.895104547298466	14.56854519710348
13	20.292667156776652	31.815733152210235	32.772908626609244	15.118691064403873
14	14.733452182414169	34.974984185404566	34.647035326775395	15.644528305405872
15	14.638144959612553	25.337049050891135	45.515882205743615	14.508923783752698
16	17.632955285494248	23.304325660987917	42.48134499442798	16.581374059089857
17	18.227055625410905	22.221130786315673	33.88762389394973	25.664189694323685
18	19.968914800129937	23.558851277524003	37.575634176933846	18.896599745412214
19	23.66973218546052	24.378474742497975	33.26654160054586	18.685251471495647
20	25.854462202728346	24.275458390128584	31.796304902291446	18.073774504851624
21	21.75078062708174	25.700776974570754	34.00957113304	18.53887126530752
22	20.787543538708068	23.060586608806748	33.660857360897616	22.49101249158757
23	17.55803995380739	27.293706956712306	33.736207885382655	21.41204520409765
24	17.49465723127233	24.548479700587357	40.6883506659227	17.268512402217617
25	16.45631830001259	26.481077661709097	37.73130885788313	19.331295180395188
26	17.30789735045299	31.55579871089676	33.062902456197065	18.073401482453182
27	16.85097599756292	30.45078202591573	34.39723466061956	18.301007315901792
28	15.053225633477515	27.008935440702647	40.46739706524628	17.47044186057355
29	16.740281600825625	23.565285913897107	39.45507644627778	20.239356038999492
30	18.608968391014514	26.699295764796943	36.05696673728188	18.634769106906663
31	24.086273863719377	25.95091957792516	31.412558109895507	18.55024844845996
32	24.362341523765412	26.489408495274276	31.975200227543667	17.173049753416652
33	23.707252021703688	27.376331417966938	30.563776727443415	18.352639832885963
34	19.326881082013635	27.317829071811474	32.27915131187315	21.07613853430174
35	19.154327137534914	27.328335869367553	33.457808835346356	20.05952815775117
36	25.09147597191763	26.318812918387362	30.186091549022137	18.403619560672873
37	19.960863733363592	30.389544182171708	31.158592026957084	18.49100005750762
38	19.904723862398257	30.714602117212962	28.92726529508403	20.45340872530475
39	19.980229812882637	29.75888764720785	30.818364514379237	19.442518025530276
40	22.04982024983175	26.919876343074918	29.800013366635948	21.230290040457387
41	17.808182557161796	26.383625560116446	30.96505557256607	24.84313631015569
42	21.01763618814628	26.694757325615914	28.588001423702153	23.69960506253565
43	20.862210188795963	27.551434348835002	29.330129485400057	22.256225976968974
44	19.715477165589306	29.71390736299587	30.15034356917156	20.420271902243265
45	18.42749299417308	31.942529682480224	28.34628290951254	21.283694413834155
46	20.812722550602818	30.26044734711133	29.82180409174486	19.10502601054099
47	20.178304706454686	27.341640334911943	30.686780863329254	21.793274095304113
48	21.326498734055235	25.457908307985942	31.940322633289455	21.27527032466937
49	20.50780782507736	25.172079895180705	33.05034403544956	21.26976824429237
50	19.095420683781143	29.711234035807045	30.647768937492327	20.545576342919492
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	667.0
1	1976.5
2	3286.0
3	31652.5
4	60019.0
5	44787.0
6	29555.0
7	29419.5
8	29284.0
9	29970.5
10	30657.0
11	30048.5
12	29440.0
13	28338.5
14	27237.0
15	25367.5
16	23498.0
17	22453.5
18	21409.0
19	20360.5
20	19312.0
21	19555.0
22	19798.0
23	22353.5
24	24909.0
25	30404.5
26	35900.0
27	41353.0
28	46806.0
29	55676.0
30	64546.0
31	76964.0
32	89382.0
33	97818.0
34	106254.0
35	121385.0
36	136516.0
37	137393.5
38	138271.0
39	163987.0
40	189703.0
41	218846.5
42	247990.0
43	273441.5
44	298893.0
45	298086.5
46	297280.0
47	282346.0
48	267412.0
49	256358.5
50	245305.0
51	218027.5
52	190750.0
53	172918.5
54	155087.0
55	133603.0
56	112119.0
57	98992.0
58	85865.0
59	75781.5
60	65698.0
61	57161.0
62	48624.0
63	39945.5
64	31267.0
65	23918.0
66	16569.0
67	13370.0
68	10171.0
69	8394.5
70	6618.0
71	5582.5
72	4547.0
73	3582.5
74	2618.0
75	2048.0
76	1478.0
77	1267.0
78	1056.0
79	903.5
80	751.0
81	467.5
82	184.0
83	179.5
84	175.0
85	104.5
86	34.0
87	22.0
88	10.0
89	9.0
90	8.0
91	5.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3216965.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.46456626310255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5002696526922	14.679379969366558
2	8.3642621221529	3.0888494439290195
3	2.7848393055667815	1.54262549669191
4	1.3698741561675778	1.0117652851467172
5	0.829322616610785	0.7656541203949716
6	0.5548243021931051	0.6146754055334532
7	0.42607768357713227	0.5507137735147442
8	0.33339965257380716	0.49248639816355455
9	0.27210772224484947	0.4521915961282734
>10	3.072166718175897	13.368321425416699
>50	1.0854716313178863	14.477779225022566
>100	1.3981366155966326	46.76768628813779
>500	0.00873405328988655	0.985362789928298
>1k	1.712559468605206E-4	0.03215923613995312
>5k	1.712559468605206E-4	0.2103473341228792
>10k+	1.712559468605206E-4	0.9600022123626712
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30359	0.9437155828552689	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6652	0.20677874953566483	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04065944143004353	0.0
2	0.0	0.0	0.0	0.145043542593718	0.0
3	0.0	0.0	0.0	0.20718285713397566	0.0
4	0.0	0.0	0.0	0.3176596574721826	0.0
5	0.0	0.0	0.0	0.5580415080673865	0.0
6	3.1085199870063864E-5	0.0	0.0	0.7847769559196324	0.0
7	3.1085199870063864E-5	0.0	0.0	0.9458293764464332	0.0
8	3.1085199870063864E-5	0.0	0.0	1.388669133795363	0.0
9	3.1085199870063864E-5	0.0	0.0	1.58674402736741	0.0
10	3.1085199870063864E-5	0.0	0.0	1.952274892639491	0.0
11	3.1085199870063864E-5	0.0	0.0	2.2335337810638287	0.0
12	3.1085199870063864E-5	0.0	0.0	2.52125839106114	0.0
13	3.1085199870063864E-5	0.0	0.0	2.6266993890203967	0.0
14	3.1085199870063864E-5	0.0	0.0	2.6655558888579765	0.0
15	3.1085199870063864E-5	0.0	0.0	2.728689929794076	0.0
16	3.1085199870063864E-5	0.0	0.0	2.86701906921586	0.0
17	3.1085199870063864E-5	0.0	0.0	3.0598405640098663	0.0
18	3.1085199870063864E-5	0.0	0.0	3.3171949337341253	0.0
19	3.1085199870063864E-5	0.0	0.0	3.440882944017109	0.0
20	3.1085199870063864E-5	0.0	0.0	3.588382217400562	0.0
21	3.1085199870063864E-5	0.0	0.0	3.7637338298675926	0.0
22	3.1085199870063864E-5	0.0	0.0	3.9701395570048166	0.0
23	3.1085199870063864E-5	0.0	0.0	4.177104817739702	0.0
24	3.1085199870063864E-5	0.0	0.0	4.326220521516398	0.0
25	3.1085199870063864E-5	0.0	0.0	4.4519601549908066	0.0
26	3.1085199870063864E-5	0.0	0.0	4.579875752456119	0.0
27	3.1085199870063864E-5	0.0	0.0	4.712329789102461	0.0
28	6.217039974012773E-5	0.0	0.0	4.864460757266554	0.0
29	6.217039974012773E-5	0.0	0.0	5.025451007393615	0.0
30	6.217039974012773E-5	0.0	0.0	5.223712412164882	0.0
31	6.217039974012773E-5	0.0	0.0	5.407332687797349	0.0
32	6.217039974012773E-5	0.0	0.0	5.566893018730387	0.0
33	6.217039974012773E-5	0.0	0.0	5.732732560037178	0.0
34	6.217039974012773E-5	0.0	0.0	5.911534629689785	0.0
35	6.217039974012773E-5	0.0	0.0	6.12798087638504	0.0
36	6.217039974012773E-5	0.0	0.0	6.316512613596977	0.0
37	6.217039974012773E-5	0.0	0.0	6.506225588403977	0.0
38	6.217039974012773E-5	0.0	0.0	6.7009432803900575	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATTCG	45	4.802132E-10	44.0	13
CGTCCGA	35	1.4471698E-7	44.0	40
TCGCGAT	25	4.4446322E-5	44.0	28
CGTTTTT	26935	0.0	43.117878	1
CCGTAAG	85	0.0	41.411766	1
TAATGCG	85	0.0	41.411766	1
TATCGCG	165	0.0	41.333332	1
TATTGCG	135	0.0	40.74074	1
TACGGGA	2105	0.0	40.551067	4
CGGTCTA	180	0.0	40.333332	31
TATTACG	110	0.0	40.0	1
TATCCGA	55	7.8216544E-11	40.0	15
TCGGTAC	100	0.0	39.6	36
GCGGATA	95	0.0	39.36842	5
CGTTAGG	510	0.0	39.254898	2
CGACGTA	45	2.3532266E-8	39.11111	21
TCGCGAG	125	0.0	38.72	1
TCTGCGA	40	4.1276144E-7	38.500004	14
CGTTTCG	160	0.0	38.500004	42
TGCGTTC	40	4.1276144E-7	38.500004	24
>>END_MODULE