FastQCFastQC Report
Thu 26 May 2016
SRR1545849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545849_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3580678
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420711.1749450802334083No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA67940.18974060219880146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT254.4447326E-544.016
CGTTTTT264050.042.7585681
CGGTCTA5300.041.9245331
ACGAGTT700.040.85714336
TACGGGA15350.040.1302954
CTACGGG7200.040.027783
TGATTCG3500.039.60000215
CACGACG5700.039.3684226
CGAGTTA452.3534085E-839.1111139
CTACCGT404.1277963E-738.50000442
CATACGA7150.038.4615418
CGACGGT5900.038.4067828
CGTAAGG5800.038.310352
TCGACAA5350.037.8317819
AAGGGAT45200.037.818585
GCGTAAG3900.037.7948721
TACGTCG357.294526E-637.71428716
TGCGTAT357.294526E-637.71428714
ACGGTAC357.294526E-637.71428713
CGACAAT5400.037.4814820
Produced by FastQC (version 0.11.5)