##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545849_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3580678
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.519827809146758	31.0	31.0	33.0	30.0	34.0
2	31.930211540942803	31.0	31.0	34.0	30.0	34.0
3	31.97736881115811	33.0	31.0	34.0	30.0	34.0
4	35.73996405150086	37.0	35.0	37.0	35.0	37.0
5	35.67512716865353	37.0	35.0	37.0	33.0	37.0
6	35.72296336057026	37.0	35.0	37.0	33.0	37.0
7	35.999645039291444	37.0	35.0	37.0	35.0	37.0
8	35.98295685900827	37.0	35.0	37.0	35.0	37.0
9	37.74053070396165	39.0	38.0	39.0	35.0	39.0
10	37.27229368292821	39.0	37.0	39.0	34.0	39.0
11	37.026818105397915	39.0	37.0	39.0	33.0	39.0
12	36.514487200468736	39.0	35.0	39.0	33.0	39.0
13	36.27297260462963	39.0	35.0	39.0	32.0	39.0
14	37.351069546046865	39.0	35.0	41.0	32.0	41.0
15	37.46310754555422	40.0	35.0	41.0	32.0	41.0
16	37.57944864073229	40.0	35.0	41.0	33.0	41.0
17	37.50645352639919	39.0	35.0	41.0	33.0	41.0
18	37.36878295116176	39.0	35.0	41.0	32.0	41.0
19	37.23409253778195	38.0	35.0	41.0	32.0	41.0
20	37.043210810913465	38.0	35.0	41.0	32.0	41.0
21	36.91050074874088	38.0	35.0	41.0	32.0	41.0
22	36.8015959547326	38.0	35.0	41.0	32.0	41.0
23	36.70678988727833	38.0	35.0	41.0	31.0	41.0
24	36.74497287943792	38.0	35.0	41.0	32.0	41.0
25	36.70538317044984	38.0	35.0	41.0	32.0	41.0
26	36.47125293031096	38.0	35.0	41.0	31.0	41.0
27	36.439506987224206	38.0	35.0	41.0	31.0	41.0
28	36.41577879943407	38.0	35.0	41.0	31.0	41.0
29	36.37010085799393	38.0	35.0	40.0	31.0	41.0
30	36.2556624750955	38.0	35.0	40.0	31.0	41.0
31	36.08535003706002	38.0	35.0	40.0	30.0	41.0
32	35.83823510519516	38.0	35.0	40.0	30.0	41.0
33	35.54102267782805	38.0	35.0	40.0	28.0	41.0
34	35.253498359807836	38.0	34.0	40.0	26.0	41.0
35	34.987470529324334	38.0	34.0	40.0	24.0	41.0
36	34.80727811883671	38.0	34.0	40.0	23.0	41.0
37	34.71966454397742	38.0	34.0	40.0	23.0	41.0
38	34.618906251832755	38.0	34.0	40.0	23.0	41.0
39	34.50253052634166	37.0	34.0	40.0	23.0	41.0
40	34.35185989915876	37.0	34.0	40.0	22.0	41.0
41	34.315598889372346	37.0	34.0	40.0	22.0	41.0
42	34.28197341397355	37.0	33.0	40.0	22.0	41.0
43	34.16385025405803	37.0	33.0	40.0	22.0	41.0
44	34.03063693523964	37.0	33.0	40.0	21.0	41.0
45	33.93263761779194	36.0	33.0	40.0	21.0	41.0
46	33.87750951076863	36.0	33.0	40.0	21.0	41.0
47	33.81765660023046	36.0	33.0	40.0	20.0	41.0
48	33.69795105843083	36.0	33.0	40.0	20.0	41.0
49	33.61798910709089	36.0	33.0	40.0	20.0	41.0
50	33.48440211602384	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	12.0
13	16.0
14	31.0
15	72.0
16	228.0
17	671.0
18	1575.0
19	3090.0
20	5508.0
21	8985.0
22	13526.0
23	20218.0
24	29769.0
25	45143.0
26	66173.0
27	84234.0
28	88110.0
29	87516.0
30	91554.0
31	104222.0
32	124958.0
33	160990.0
34	272787.0
35	432258.0
36	246027.0
37	311965.0
38	487199.0
39	893831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.826235143176795	21.97941283745704	28.476087489575995	17.71826452979017
2	34.52212122955485	24.92860290704721	28.25300124724982	12.296274616148114
3	27.854864358090843	24.24429116496932	34.812289739540944	13.088554737398894
4	25.41367863851483	26.149405224373705	33.93695272236152	14.499963414749944
5	22.48585323785049	31.283712190819728	32.656245549027304	13.57418902230248
6	20.9546907038276	39.00576929844013	29.687143049444824	10.352396948287447
7	83.25540581979168	4.2321593843400604	10.740982573691351	1.7714522221769173
8	84.39851335417482	3.377405061276105	10.557721191349795	1.6663603931992768
9	78.86389114016954	6.380998235529696	11.971643359162707	2.783467265138055
10	39.60043321404494	32.83266465177824	16.78254788618245	10.784354247994374
11	37.4333855208427	21.04646103335737	27.237551100657477	14.282602345142456
12	34.47768830372348	20.874063515345416	29.161125351120653	15.487122829810444
13	22.492779300456505	30.477747510387697	30.68460777539896	16.344865413756835
14	17.156694905266544	33.624079015203264	32.331335015323916	16.887891064206276
15	16.734121303283903	23.269196504125755	43.35514112131836	16.641541071271977
16	19.151037876067043	20.59372554583238	41.827274052567695	18.427962525532873
17	19.189717701507927	20.522174850684703	32.3625302247228	27.92557722308457
18	21.285242627234283	23.072501911649134	35.3350678279365	20.307187633180085
19	26.008873179883814	22.86078223174494	30.91475971869015	20.215584869681106
20	27.77627588964995	22.338953684190535	30.432588465089573	19.452181961069943
21	23.582321560330193	24.698618529786817	31.44518998915848	20.27386992072451
22	22.764431764040218	22.929791508758957	30.539579375749508	23.766197351451318
23	20.7557618976071	26.467054563409498	30.163170215249735	22.614013323733662
24	20.594395809955547	23.612762722590528	37.05792590118408	18.73491556626985
25	19.327345268130784	24.926592114677725	35.11025006995882	20.635812547232675
26	18.92124899250924	29.781147592718476	30.80564630497353	20.491957109798758
27	19.43229745874943	28.899806126102373	32.344907863817966	19.322988551330223
28	17.700474602854545	26.162754651493376	37.35203221289376	18.784738532758322
29	19.155562158898398	23.472845086880195	36.172255645439215	21.19933710878219
30	20.086140110895197	26.83815746626756	33.29104152900651	19.784660893830722
31	25.69851296318742	25.14493065279816	29.29961867556926	19.85693770844516
32	26.50556682281959	25.27806186426146	30.220952568200772	17.99541874471818
33	25.212403907863255	26.779537283162576	28.533087867716674	19.474970941257492
34	21.137924158497356	27.03348360282606	30.497855434082595	21.330736804593993
35	21.994437924884615	25.798438172882342	30.29644106507203	21.91068283716101
36	25.694714799822826	26.79475786429274	28.354518334237262	19.156009001647174
37	22.19130008339203	30.176352076338613	28.824261773887518	18.80808606638184
38	21.93053941180972	29.767211684491034	26.8301701521332	21.472078751566045
39	22.01988003389302	28.850346219347283	28.44776883037235	20.682004916387342
40	23.298939474591123	26.93506648740825	28.225604201215525	21.540389836785103
41	19.24428837220214	26.71670002161602	28.8227257519386	25.216285854243246
42	22.07671284600291	27.279135403965395	26.484648996642534	24.159502753389162
43	22.8505327761949	26.64165836749353	27.709528754051604	22.798280102259962
44	21.51145676880189	28.697972842014835	28.494324259260395	21.29624612992288
45	19.61335255501891	31.59331836037756	26.63942415374965	22.153904930853876
46	21.507183835016722	30.227264222027223	28.354490406565457	19.911061536390594
47	22.455551713949145	27.077469685908646	29.312046489519584	21.154932110622624
48	22.14547077397074	25.10778684930619	30.598199558854493	22.14854281786857
49	20.7320233765784	25.677762703041157	31.63403690585973	21.956177014520716
50	20.409738044024063	28.551631841790858	29.452466823322286	21.586163290862793
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1277.0
1	4070.5
2	6864.0
3	41732.0
4	76600.0
5	54644.5
6	32689.0
7	31820.0
8	30951.0
9	30775.5
10	30600.0
11	29274.0
12	27948.0
13	26472.0
14	24996.0
15	23450.0
16	21904.0
17	20594.0
18	19284.0
19	18235.5
20	17187.0
21	17677.0
22	18167.0
23	19324.0
24	20481.0
25	24651.5
26	28822.0
27	36328.0
28	43834.0
29	51301.5
30	58769.0
31	65801.0
32	72833.0
33	76863.0
34	80893.0
35	94956.5
36	109020.0
37	115663.5
38	122307.0
39	137947.0
40	153587.0
41	191764.0
42	229941.0
43	256741.5
44	283542.0
45	284112.5
46	284683.0
47	269431.5
48	254180.0
49	260675.5
50	267171.0
51	255738.0
52	244305.0
53	224673.5
54	205042.0
55	189492.0
56	173942.0
57	161470.5
58	148999.0
59	144280.0
60	139561.0
61	125187.5
62	110814.0
63	97812.0
64	84810.0
65	69687.0
66	54564.0
67	44545.5
68	34527.0
69	30089.5
70	25652.0
71	20106.5
72	14561.0
73	12635.0
74	10709.0
75	8861.5
76	7014.0
77	5706.5
78	4399.0
79	3009.0
80	1619.0
81	1225.0
82	831.0
83	593.5
84	356.0
85	282.0
86	208.0
87	167.0
88	126.0
89	90.0
90	54.0
91	48.0
92	42.0
93	25.0
94	8.0
95	6.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3580678.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.575126736352995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2485075920674	13.301291837417029
2	7.862260546028102	2.606359299692874
3	2.5616502151258445	1.2737903090975036
4	1.2857382105678319	0.8524509515973413
5	0.7558867651648082	0.6264459465469296
6	0.5172699952041859	0.5144289436453252
7	0.3727159617830571	0.43244700122511104
8	0.3033961679571504	0.4023063948170887
9	0.25878547012306063	0.38604617683347797
>10	2.934493054693358	11.696255194066808
>50	1.243627808264291	15.038606298740362
>100	1.6402666842319928	49.528096637314846
>500	0.013005735421854703	1.360512790796097
>1k	0.0020535371718717953	0.5949211982520033
>5k	1.7112809765598296E-4	0.1927097654679179
>10k+	1.7112809765598296E-4	1.1933312544893693
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42071	1.1749450802334083	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6794	0.18974060219880146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020247562053890354	0.0
2	0.0	0.0	0.0	0.0858496631084951	0.0
3	0.0	0.0	0.0	0.13553299123797224	0.0
4	0.0	0.0	0.0	0.2076143121498219	0.0
5	0.0	0.0	0.0	0.3668020414010978	0.0
6	0.0	0.0	0.0	0.5221078242723864	0.0
7	0.0	0.0	0.0	0.6330365366559071	0.0
8	0.0	0.0	0.0	0.9056106134089689	0.0
9	0.0	0.0	0.0	1.043992227170385	0.0
10	0.0	0.0	0.0	1.2904260031200796	0.0
11	0.0	0.0	0.0	1.527113021612108	0.0
12	0.0	0.0	0.0	1.7344759847157438	0.0
13	0.0	0.0	0.0	1.814740113464545	0.0
14	0.0	0.0	0.0	1.844790288319698	0.0
15	0.0	0.0	0.0	1.8948646038543537	0.0
16	0.0	0.0	0.0	2.0091446368536907	0.0
17	0.0	0.0	0.0	2.1388686723575816	0.0
18	0.0	0.0	0.0	2.3235822936326582	0.0
19	0.0	0.0	0.0	2.407616658074253	0.0
20	0.0	0.0	0.0	2.5067598929588195	0.0
21	0.0	0.0	0.0	2.621905683783909	0.0
22	0.0	0.0	0.0	2.7463793169896875	0.0
23	0.0	0.0	0.0	2.8802366479197516	0.0
24	0.0	0.0	0.0	2.981809590250785	0.0
25	0.0	0.0	0.0	3.069502479697979	0.0
26	0.0	0.0	0.0	3.1547098063551093	0.0
27	0.0	0.0	0.0	3.236314463350237	0.0
28	0.0	0.0	0.0	3.329006406049357	0.0
29	0.0	0.0	0.0	3.425608222800263	0.0
30	0.0	0.0	0.0	3.547205305810799	0.0
31	0.0	0.0	0.0	3.6608430023587712	0.0
32	0.0	0.0	0.0	3.7694816456548175	0.0
33	0.0	0.0	0.0	3.8670050755750727	0.0
34	0.0	0.0	0.0	3.979218460861323	0.0
35	0.0	0.0	0.0	4.119973926725609	0.0
36	0.0	0.0	0.0	4.243023248669665	0.0
37	0.0	0.0	0.0	4.36811129065501	0.0
38	0.0	0.0	0.0	4.492501140845393	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	25	4.4447326E-5	44.0	16
CGTTTTT	26405	0.0	42.758568	1
CGGTCTA	530	0.0	41.92453	31
ACGAGTT	70	0.0	40.857143	36
TACGGGA	1535	0.0	40.130295	4
CTACGGG	720	0.0	40.02778	3
TGATTCG	350	0.0	39.600002	15
CACGACG	570	0.0	39.36842	26
CGAGTTA	45	2.3534085E-8	39.11111	39
CTACCGT	40	4.1277963E-7	38.500004	42
CATACGA	715	0.0	38.46154	18
CGACGGT	590	0.0	38.40678	28
CGTAAGG	580	0.0	38.31035	2
TCGACAA	535	0.0	37.83178	19
AAGGGAT	4520	0.0	37.81858	5
GCGTAAG	390	0.0	37.794872	1
TACGTCG	35	7.294526E-6	37.714287	16
TGCGTAT	35	7.294526E-6	37.714287	14
ACGGTAC	35	7.294526E-6	37.714287	13
CGACAAT	540	0.0	37.48148	20
>>END_MODULE