FastQCFastQC Report
Thu 26 May 2016
SRR1545848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545848_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences602632
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50860.8439644758326807No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21140.35079451472872336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC207.853496E-444.00000440
CTCACGA408.301868E-944.00000424
AGTCCGA207.853496E-444.00000434
GCAAGCG207.853496E-444.0000041
GTACACG207.853496E-444.00000427
CGATTCA207.853496E-444.00000410
CCCGTCA207.853496E-444.00000422
TCTACGG207.853496E-444.0000042
CGGATCC207.853496E-444.00000424
CTACGCC207.853496E-444.00000416
GTCCGTT207.853496E-444.0000049
CATACCG207.853496E-444.00000441
TTTATCG207.853496E-444.0000041
CCGAACT207.853496E-444.00000434
TTGCACG207.853496E-444.00000426
GCTCGAT207.853496E-444.00000426
ATACCGG207.853496E-444.00000416
CTATGCG351.4445504E-744.01
TCGTTGA351.4445504E-744.024
CAACGTA454.783942E-1044.028
Produced by FastQC (version 0.11.5)