##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545848_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	602632
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48067311394018	31.0	31.0	33.0	30.0	34.0
2	31.880376415457526	31.0	31.0	34.0	30.0	34.0
3	31.95713138366366	33.0	31.0	34.0	30.0	34.0
4	35.72307311925022	37.0	35.0	37.0	33.0	37.0
5	35.646372910831154	37.0	35.0	37.0	33.0	37.0
6	35.694695933836904	37.0	35.0	37.0	33.0	37.0
7	35.962287100585435	37.0	35.0	37.0	35.0	37.0
8	35.95214492426555	37.0	35.0	37.0	35.0	37.0
9	37.715839849194865	39.0	38.0	39.0	35.0	39.0
10	37.26826155929324	39.0	37.0	39.0	34.0	39.0
11	36.98325180209481	39.0	37.0	39.0	33.0	39.0
12	36.299240664285996	38.0	35.0	39.0	32.0	39.0
13	35.97014761911083	38.0	35.0	39.0	32.0	39.0
14	37.0148614743326	39.0	35.0	41.0	32.0	41.0
15	37.17675463632864	39.0	35.0	41.0	32.0	41.0
16	37.31664266086102	39.0	35.0	41.0	33.0	41.0
17	37.25512916672198	39.0	35.0	41.0	32.0	41.0
18	37.13678662931939	39.0	35.0	41.0	32.0	41.0
19	37.029540416041634	38.0	35.0	41.0	32.0	41.0
20	36.86062472620107	38.0	35.0	41.0	32.0	41.0
21	36.69943680388695	38.0	35.0	41.0	32.0	41.0
22	36.59682028169762	38.0	35.0	41.0	31.0	41.0
23	36.50366890573351	38.0	35.0	40.0	31.0	41.0
24	36.53116827516627	38.0	35.0	40.0	31.0	41.0
25	36.52064941788687	38.0	35.0	40.0	31.0	41.0
26	36.286883205671124	38.0	35.0	40.0	31.0	41.0
27	36.26166217525787	38.0	35.0	40.0	31.0	41.0
28	36.25256707244222	38.0	35.0	40.0	31.0	41.0
29	36.20749810829827	38.0	35.0	40.0	31.0	41.0
30	36.11672629399036	38.0	35.0	40.0	30.0	41.0
31	35.94868676074287	38.0	35.0	40.0	30.0	41.0
32	35.735779049237344	38.0	35.0	40.0	30.0	41.0
33	35.50021240159832	38.0	35.0	40.0	29.0	41.0
34	35.26778365569701	37.0	34.0	40.0	27.0	41.0
35	35.019964090854785	37.0	34.0	40.0	25.0	41.0
36	34.82062187205459	37.0	34.0	40.0	24.0	41.0
37	34.72866193630607	37.0	34.0	40.0	24.0	41.0
38	34.678039002243494	37.0	34.0	40.0	24.0	41.0
39	34.57961741162102	37.0	34.0	40.0	23.0	41.0
40	34.446280648886884	37.0	34.0	40.0	23.0	41.0
41	34.42099158358667	37.0	34.0	40.0	23.0	41.0
42	34.37711903782076	36.0	34.0	40.0	23.0	41.0
43	34.29003936067119	36.0	33.0	40.0	23.0	41.0
44	34.168396301557166	36.0	33.0	40.0	23.0	41.0
45	34.05432170877086	36.0	33.0	40.0	23.0	41.0
46	34.01977823945625	36.0	33.0	40.0	23.0	41.0
47	33.97421311845372	35.0	33.0	40.0	23.0	41.0
48	33.8785942332966	35.0	33.0	40.0	23.0	41.0
49	33.828474093642555	36.0	33.0	40.0	22.0	41.0
50	33.66170565121003	35.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	9.0
15	14.0
16	43.0
17	117.0
18	274.0
19	535.0
20	933.0
21	1516.0
22	2271.0
23	3396.0
24	4920.0
25	7345.0
26	10501.0
27	13420.0
28	13957.0
29	14214.0
30	15402.0
31	17751.0
32	21788.0
33	28252.0
34	50492.0
35	84655.0
36	39726.0
37	49724.0
38	77778.0
39	143596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.212142733874074	22.1543163987309	27.659168447742573	16.974372419652457
2	32.02966321071566	24.320314885369513	30.375585763782876	13.274436140131954
3	27.436976463247888	24.24663808095156	34.941058556465634	13.375326899334915
4	25.36257616588565	26.836112254244714	33.45773208193392	14.343579497935721
5	22.515233177129655	32.17618712580812	32.07596012159992	13.232619575462307
6	20.97349626305938	40.01098514516322	29.022521206972087	9.99299738480532
7	85.38643815794714	4.247368211445791	8.961190245456597	1.4050033851504733
8	86.80023629677814	3.0091332687278474	8.684238473894517	1.5063919605995035
9	81.42216145176494	6.079166058224588	10.109652325133748	2.3890201648767406
10	42.91939359343679	33.373601136348555	14.389710470071288	9.31729480014337
11	40.92829454791647	20.277051334811294	25.234969268143743	13.55968484912849
12	37.72982516693438	20.127872399739807	27.812661790279975	14.329640643045838
13	21.602570059339694	36.02297919791847	27.57951784837181	14.79493289437003
14	16.67518485576604	37.636733528919805	29.808407120763587	15.879674494550573
15	15.864076252173797	23.113442366153805	46.16913804776381	14.853343333908587
16	17.871935111311714	19.983007872134237	44.16277263736409	17.982284379189952
17	17.760922088438715	19.935051573763094	31.003829866319744	31.300196471478447
18	21.724203162128795	22.160290193683707	35.99244646815967	20.123060176027824
19	27.047352281325914	23.039433684238475	29.762773964874086	20.150440069561522
20	30.08253793359795	22.385303136906103	28.14105457393567	19.391104355560277
21	22.67785315084496	26.13767606101236	30.66564669649139	20.51882409165129
22	22.754516852739318	23.093861593808494	29.381944536632638	24.769677016819553
23	20.01121745941138	28.07003278949674	29.21816299167651	22.700586759415366
24	20.35786350542288	23.66867341926748	38.946819949820124	17.026643125489517
25	17.474677746950046	24.837214087536008	36.280350197135235	21.407757968378714
26	18.90838853562373	31.89176811055503	29.32403191334015	19.87581144048109
27	18.957672343984388	30.170319531654478	31.514921212282122	19.357086912079012
28	16.814241527167493	26.711492253979213	38.462776619894065	18.011489598959233
29	17.247839477492068	23.386743485244725	38.04710005442791	21.318316982835295
30	19.24507825671388	28.179884241128917	32.97833503697115	19.59670246518605
31	27.12916008442964	25.18618327602915	28.039002243491883	19.64565439604933
32	27.230548659878668	26.112951187457682	28.956809462491208	17.699690690172442
33	25.58144937540655	26.396872386464707	28.393447410691763	19.628230827436976
34	19.948160734909532	26.56612991012757	30.66415324775319	22.821556107209705
35	21.26488470575741	24.928480399315006	31.746239827954703	22.06039506697288
36	27.609718700633223	25.286908096483423	28.258871085504918	18.844502117378433
37	20.9975573816193	30.720406483558786	29.654250023231427	18.62778611159049
38	20.348902812993668	31.93690345019846	25.902540854119927	21.811652882687945
39	21.06758353356609	30.60541093071725	28.248251005588816	20.07875453012784
40	23.289171501015545	26.910286874908735	27.09713390593264	22.70340771814308
41	18.362284113687956	25.36191241089089	28.819080301079264	27.456723174341885
42	21.827417063813407	26.557003278949676	26.03628748556333	25.57929217167359
43	23.016368198170692	26.615745595985608	26.79396381207769	23.573922393766015
44	20.559313146331426	29.9723546044684	28.70624195197069	20.762090297229488
45	18.83189077247806	34.04598494603672	25.02024452734007	22.101879754145152
46	21.34420342763079	30.898126883404796	27.816146504002443	19.94152318496197
47	21.91138207065008	27.49986724900105	28.316949647546096	22.27180103280277
48	22.94385296499356	24.541013421125992	30.65303535159102	21.86209826228942
49	20.73255319996283	24.59942386066455	32.270606273812206	22.397416665560407
50	19.313113143676404	31.141559027731684	28.758014841561685	20.787312987030226
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192.0
1	521.0
2	850.0
3	5215.5
4	9581.0
5	7020.5
6	4460.0
7	4438.0
8	4416.0
9	4461.5
10	4507.0
11	4425.5
12	4344.0
13	4001.0
14	3658.0
15	3463.5
16	3269.0
17	2971.5
18	2674.0
19	2493.0
20	2312.0
21	2430.5
22	2549.0
23	2665.0
24	2781.0
25	3197.0
26	3613.0
27	5242.5
28	6872.0
29	7897.0
30	8922.0
31	10330.5
32	11739.0
33	13634.0
34	15529.0
35	16160.0
36	16791.0
37	19543.0
38	22295.0
39	24798.5
40	27302.0
41	35558.5
42	43815.0
43	50179.5
44	56544.0
45	57461.0
46	58378.0
47	54897.5
48	51417.0
49	48610.0
50	45803.0
51	42202.5
52	38602.0
53	36438.5
54	34275.0
55	30045.5
56	25816.0
57	23803.5
58	21791.0
59	20265.0
60	18739.0
61	17288.5
62	15838.0
63	13412.5
64	10987.0
65	9149.5
66	7312.0
67	6156.5
68	5001.0
69	4039.0
70	3077.0
71	2889.0
72	2701.0
73	2398.0
74	2095.0
75	1455.5
76	816.0
77	675.5
78	535.0
79	391.0
80	247.0
81	183.5
82	120.0
83	80.0
84	40.0
85	25.0
86	10.0
87	9.5
88	9.0
89	5.0
90	1.0
91	2.0
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	602632.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.054064343097394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08520158188972	17.000419955876286
2	5.511485595278906	2.4310131588867088
3	2.2412917906477965	1.4828878008780744
4	1.408708123289646	1.242709583666953
5	1.0728039439263644	1.1829843603440344
6	0.8199855947339482	1.0850409040005287
7	0.7123266433503211	1.09967883380254
8	0.6623966360696928	1.1686830426025818
9	0.5310926579729423	1.0541476485973695
>10	8.092161340361532	42.6737087617117
>50	1.76824951023538	25.607644444636872
>100	0.09203346426993017	2.675559628109734
>500	7.543726579502474E-4	0.09765909101482487
>1k	7.543726579502474E-4	0.3517058235184664
>5k	7.543726579502474E-4	0.8461569623533208
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5086	0.8439644758326807	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2114	0.35079451472872336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03268993349174953	0.0
2	0.0	0.0	0.0	0.09906543296738309	0.0
3	0.0	0.0	0.0	0.1420435688778558	0.0
4	0.0	0.0	0.0	0.20742343586135487	0.0
5	0.0	0.0	0.0	0.3459822910167399	0.0
6	0.0	0.0	0.0	0.4739210662560236	0.0
7	0.0	0.0	0.0	0.5691699080035577	0.0
8	0.0	0.0	0.0	0.870514675622934	0.0
9	0.0	0.0	0.0	0.9921477784120325	0.0
10	0.0	0.0	0.0	1.1851345431374372	0.0
11	0.0	0.0	0.0	1.3298331319943182	0.0
12	0.0	0.0	0.0	1.48349241328041	0.0
13	0.0	0.0	0.0	1.5414050365729002	0.0
14	0.0	0.0	0.0	1.5621473801590358	0.0
15	0.0	0.0	0.0	1.5948373136507852	0.0
16	0.0	0.0	0.0	1.6736582192781	0.0
17	0.0	0.0	0.0	1.7737192847376178	0.0
18	0.0	0.0	0.0	1.9293698310079783	0.0
19	0.0	0.0	0.0	1.9906012292742503	0.0
20	0.0	0.0	0.0	2.059631748728909	0.0
21	0.0	0.0	0.0	2.1416054905813167	0.0
22	0.0	0.0	0.0	2.2181032537269845	0.0
23	0.0	0.0	0.0	2.318164319186502	0.0
24	0.0	0.0	0.0	2.4024612035205566	0.0
25	0.0	0.0	0.0	2.4645222955302737	0.0
26	0.0	0.0	0.0	2.5284087137755713	0.0
27	0.0	0.0	0.0	2.5976051719789193	0.0
28	0.0	0.0	0.0	2.6687928951665363	0.0
29	0.0	0.0	0.0	2.7431334545792456	0.0
30	0.0	0.0	0.0	2.838548235075469	0.0
31	0.0	0.0	0.0	2.9328014443308685	0.0
32	0.0	0.0	0.0	3.021080858633461	0.0
33	0.0	0.0	0.0	3.1063733754596505	0.0
34	1.659387486890839E-4	0.0	0.0	3.194320912264865	0.0
35	1.659387486890839E-4	0.0	0.0	3.3046701801431055	0.0
36	1.659387486890839E-4	0.0	0.0	3.406390633089514	0.0
37	1.659387486890839E-4	0.0	0.0	3.5079451472872334	0.0
38	1.659387486890839E-4	0.0	0.0	3.6043555602755912	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.853496E-4	44.000004	40
CTCACGA	40	8.301868E-9	44.000004	24
AGTCCGA	20	7.853496E-4	44.000004	34
GCAAGCG	20	7.853496E-4	44.000004	1
GTACACG	20	7.853496E-4	44.000004	27
CGATTCA	20	7.853496E-4	44.000004	10
CCCGTCA	20	7.853496E-4	44.000004	22
TCTACGG	20	7.853496E-4	44.000004	2
CGGATCC	20	7.853496E-4	44.000004	24
CTACGCC	20	7.853496E-4	44.000004	16
GTCCGTT	20	7.853496E-4	44.000004	9
CATACCG	20	7.853496E-4	44.000004	41
TTTATCG	20	7.853496E-4	44.000004	1
CCGAACT	20	7.853496E-4	44.000004	34
TTGCACG	20	7.853496E-4	44.000004	26
GCTCGAT	20	7.853496E-4	44.000004	26
ATACCGG	20	7.853496E-4	44.000004	16
CTATGCG	35	1.4445504E-7	44.0	1
TCGTTGA	35	1.4445504E-7	44.0	24
CAACGTA	45	4.783942E-10	44.0	28
>>END_MODULE