FastQCFastQC Report
Thu 26 May 2016
SRR1545847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545847_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5620197
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1071671.9068192805341166No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA105770.18819625006027368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGA302.529483E-644.021
CGTTTTT575750.042.9148061
CGGTCTA5800.042.86207231
CGACGGT5900.042.13559328
CGTACTA603.6379788E-1240.33333221
TACGCGG3900.040.051282
TACGGGA17250.038.1333354
TCGATAG4350.037.9310381
GTTTTTT674100.037.9068372
TTCGATC357.295266E-637.71428723
AGGGCGA43800.037.420096
ACGGGAC13850.036.8519865
TGCGTAG3350.036.7761151
CGACAAT8650.036.36994620
CGACAGG8350.036.359282
GCGATAT5650.036.212399
ACGGTCT6700.036.119430
CGAATAT4950.036.014
GGGCGAT89450.035.7607577
AACGGGA20050.035.6608474
Produced by FastQC (version 0.11.5)